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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #29333192 -

Proposition 122 said:
B Traven said:
Quick question:

I have this spore syringe that appears to be really dirty.

How many minutes should I vent my PC in order to make it work?


Misplaced Causality


Well, yeah, that's definitely one way to take everything I've typed out these past few posts and distill it down to two words.


--------------------


ACAB  🖕🧊


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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29333193 -

BeefSupremeJr said:
"voila" he had an led lamp making direct contact with  the too of the sab.  makes horrible draft.  dude moved the lamp and hes been a canopy machine ever since.  sure. physics i guess but i learned that one in grade school making hot air balloons from rice paper.


How big was the lamp?

I put a small led book light on mine. I don't think it generates any significant heat. I don't really have a contam problem on my plates as far as I know. I occasionally get bacteria blobs, but not that often. The only time I see mold on my plates is if I open a sporulated swab plate and do other work afterwards. I usually just wash my SAB with soap and water to get as much out as possible if that happens.


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Re: Ask Quick Questions, Get Quick Answers [Re: B Traven] * 1
    #29333194 -

B Traven said:
Proposition 122 said:
B Traven said:
Quick question:

I have this spore syringe that appears to be really dirty.

How many minutes should I vent my PC in order to make it work?


Misplaced Causality


Well, yeah, that's definitely one way to take everything I've typed out these past few posts and distill it down to two words.


Are you having a bad day. You seem kind of down today?:heart:


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"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #29333197 -

Proposition 122 said:
B Traven said:
Proposition 122 said:
B Traven said:
Quick question:

I have this spore syringe that appears to be really dirty.

How many minutes should I vent my PC in order to make it work?


Misplaced Causality


Well, yeah, that's definitely one way to take everything I've typed out these past few posts and distill it down to two words.


Are you having a bad day. You seem kind of down today?:heart:


Nah, I'm chillin.

I was just saying: you've made my point for me, in a far more succinct way than I did.


--------------------


ACAB  🖕🧊


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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon]
    #29333203 -

PuffM4gicDr4gon said:
BeefSupremeJr said:
"voila" he had an led lamp making direct contact with  the too of the sab.  makes horrible draft.  dude moved the lamp and hes been a canopy machine ever since.  sure. physics i guess but i learned that one in grade school making hot air balloons from rice paper.


How big was the lamp?

I put a small led book light on mine. I don't think it generates any significant heat. I don't really have a contam problem on my plates as far as I know. I occasionally get bacteria blobs, but not that often. The only time I see mold on my plates is if I open a sporulated swab plate and do other work afterwards. I usually just wash my SAB with soap and water to get as much out as possible if that happens.


I have one of those flexible little LED desk lamps from Ikea. Usually just pointing upwards like a mini floor lamp, but sometimes angled to point more directlly at my work. But it sits several inches away from my SAB, and never comes in direct contact with the outside wall.


--------------------


ACAB  🖕🧊


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Re: Ask Quick Questions, Get Quick Answers [Re: humble student] * 3
    #29333204 -

humble student said:





























First is Aspergillus niger and penicillium, second is A. niger, third is A. niger and penicillium, fourth is Trichoderma, fifth is A. niger, sixth is penicillium.

Got a nice cellulase or citric acid farm started there. :thumbup:


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Question everything, it's very responsive.

Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: B Traven] * 1
    #29333207 -

ya metas was just a little reading lamp. 
led's in general make a bunch of heat themselves but the real issue is transformers that are pulling high voltage and converting it to low voltage.  they dump all that extra voltage off as heat. 

put your hand on an led lightbulb.  bulb itself doesnt make much heat but the back of the bulb where the transformation happens does.


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29333208 -

BeefSupremeJr said:
ya metas was just a little reading lamp. 
led's in general make a bunch of heat themselves but the real issue is transformers that are pulling high voltage and converting it to low voltage.  they dump all that extra voltage off as heat. 

put your hand on an led lightbulb.  bulb itself doesnt make much heat but the back of the bulb where the transformation happens does.


Huh.
Wild.
Thanks for the applied physics lesson!


--------------------


ACAB  🖕🧊


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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #29333209 -

all the contams are happening at the edge of the plate.  theres a draft in that sab.


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #29333210 -

They replaced all the streetlights in my city with LED. I feel bad for the trees.


--------------------
Question everything, it's very responsive.

Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29333211 -

Nichrome said:
humble student said:





























First is Aspergillus niger and penicillium, second is A. niger, third is A. niger and penicillium, fourth is Trichoderma, fifth is A. niger, sixth is penicillium.

Got a nice cellulase or citric acid farm started there. :thumbup:


:rockon: thats awesome u know contams names :thumbup:


--------------------
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http://www.shroomery.org/forums/showflat.php/Number/11917410
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Re: Ask Quick Questions, Get Quick Answers [Re: tripdawg420] * 3
    #29333212 -

I love mold.


--------------------
Question everything, it's very responsive.

Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: B Traven] * 1
    #29333221 -

B Traven said:
Quick question:

I have this spore syringe that appears to be really dirty.

How many minutes should I vent my PC in order to make it work?




About 3.15 minutes, but only if Venus is in retrograde at 15k feet altitude while the moon is in Uranus.


-Kingboomer


--------------------


I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

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Re: Ask Quick Questions, Get Quick Answers [Re: kingboomer] * 1
    #29333224 -

:cantarguewiththat:


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

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The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #29333225 -

Smellyhobbit said:
Quick question:

If none of my agar is performing from spore, LC, or live myc, what’s the most likely problem? It is MEA with a 8:8:400 malt:agar:water ratio.



Needs more cowbell...

:cowbell:


--------------------


I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

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Re: Ask Quick Questions, Get Quick Answers [Re: kingboomer]
    #29333237 -

Any ideas why my agar wouldn't have set properly?

I did 5gs lme×10g agar× 500ml

I poured them and went to bed came back and they looked like they were set but then once you move them around. Some like  a "broken sauce" a  watery jelly. And some are kind of like apple sauce consistency.

The applesaucey ones had a lot of sediment and stuff when I poured. The other ones had almost none.

I'm thinking maybe for some reason everything didn't emulsify properly in the pot?






The only thing I can think of that might have been a little bit different than last time. I put my pot of water on the stove dissolved the lme.

Then I turned on the heat low weighed.  out my Agar and added that. So the water was just a little bit warm. I don't know if that makes a difference or not?


When I put everything in the pot how long does it need to stay on the stove?

Does it need to come to a boil or does it just need to be nice and hot for a certain amount of time before I take it off?


Im about to redo them now.


Ps. Not telephone brand


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
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Edited by callmehippie (09/02/25 07:43 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 1
    #29333244 -

bummer.  recipe is good. i assume youre deliberately trying to make it low nute which is fine.

i always have used telephone but i dont think it matters as much as some folks say.  i mix the ingredients cold in a blender (vitamix) first then bring to a boil until its cloudy and i dont see any specs of agar stick to the sides of the pot when i stir.  i always stir as i go like im makin a badass marinara. 

hope that helps bud.  the only thing i can think is that you didnt have a good homogenization since you have results all over the place.

try try try again.


--------------------
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Edited by BeefExtreme (09/02/25 07:53 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29333246 -

BeefSupremeJr said:
bummer.  recipe is good. i assume youre deliberately trying to make it low nute which is fine.

i always have used telephone.  i mix the ingredients cold in a blender (vitamix) first then bring to a boil until its cloudy and i dont see any specs of agar stick to the sides of the pot when i stir.  i always stir as i go like im makin a badass marinara. 

hope that helps bud.  the only thing i can think is that you didnt have a good homogenization since you have results all over the place.

try try try again.



:whathesaid:

I generally do the same, definitely stir it constantly so it doesn't boil over.  Usually 5 minutes at boil does the trick for me.


--------------------


I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

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Re: Ask Quick Questions, Get Quick Answers [Re: kingboomer] * 2
    #29333249 -

i believe i wrote somewhere never to add agar in hot water because it makes a hydrophobic mess.


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29333250 -

Alrighty.


Yes low nutes on purpose.


I have no clue about telephone. Just pointing out it wasn't telephone in case anybody planned on asking.

Does ambient room temperature that you're working and have any effect? It is a little warm in here. not uncomfortable or anything but definitely  on the warmer side.(79-80°)

The reason I asked that is because I used my immersion blender in the pot but I also did this while it was hot. I'll go ahead and give Cold to try.

Immersion  blender makes lots of foam.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   Unfolding Nature Unfolding Nature: Being in the Implicate Order

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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