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LadysKnight
Hello Ladies

Registered: 10/09/15
Posts: 3,349
Loc: Between me and you

Re: Ask Quick Questions, Get Quick Answers

[Re: humble student]
#29332412 - 09/01/25 07:07 PM (1 year, 2 months ago)

humble student said:

I poured the plates before going into the PC, and I wrapped the plates in aluminum foil in stacks.

I bought the plates and prepped the agar, and poured the plates.

What's difficult for me to understand is why the contamination problems are happening now.

This is a plate I poured previously, and inoculated with a transfer from an earlier plate, does it look contaminated?

Yep, I noticed they were glass and edited my reply. Sorry I misunderstood you earlier.
That plate looks good.
Maybe you had a fan or the AC on this last time. :shrug:

:shrug:

--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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BeefExtreme
Meatkeeper

Registered: 11/03/11
Posts: 14,589
Loc:

Last seen: 5 hours, 32 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: tryptkaloids]

1
#29332424 - 09/01/25 07:17 PM (1 year, 2 months ago)

tryptkaloids said:

Quote:

BeefSupremeJr said:l

man, you could split the hair of a frog

Sure. The main point im trying to drive is hay has seeds.

Also, wheat grass is just young wheat.

Okay im done. I just like clearly defined terms.

okay but youre doing the opposite if im understandind right. youre trying to say two totally different things are the same and they are not. hay and straw have a lot more that sets them apart than just "seeds". comparing apples to artichokes here. i dont remember anyone arguing that hay didnt have seeds but i still dont see what point youre trying to arrive at. sometimes i feel like if i say something is blue, youll show up just to say its red because its me who said it.

ill admit that i might be both misunderstanding what youre saying and that we could possibly be agreeing on a thing, and also maybe im reading into things a bit much and youre just this argumentative with everyone.

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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion
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The Hitchhiker's Guide to the Shroomery

Edited by BeefExtreme (09/01/25 07:26 PM)

Extras:

humble student
Stranger

Registered: 04/29/25
Posts: 73
Last seen: 10 months, 28 days

Re: Ask Quick Questions, Get Quick Answers

[Re: humble student]
#29332430 - 09/01/25 07:26 PM (1 year, 2 months ago)

After all of these plates were PCed, the lids never came off. They were moved to the SAB, unwrapped from the aluminum foil, and then cling-wrapped. They were ready to use, or, I should say, I thought they were ready to use.

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humble student
Stranger

Registered: 04/29/25
Posts: 73
Last seen: 10 months, 28 days

Re: Ask Quick Questions, Get Quick Answers

[Re: LadysKnight]
#29332441 - 09/01/25 07:33 PM (1 year, 2 months ago)

I'm careful to turn off all HVAC systems, every window and door is closed, the SAB arm holes are covered with cling-wrap when not in use, I wear an N95 mask, gloves.

Bottom line is, it's something that I'm doing, or not doing.

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B Traven
Stranger

Registered: 03/10/20
Posts: 5,581
Loc: Central Megalopolis
Last seen: 4 days, 21 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: tryptkaloids]
#29332454 - 09/01/25 07:43 PM (1 year, 2 months ago)

tryptkaloids said:

Quote:

BeefSupremeJr said:l

man, you could split the hair of a frog

Sure. The main point im trying to drive is hay has seeds.

Also, wheat grass is just young wheat.

Okay im done. I just like clearly defined terms.

I mean, I could write a treatise on the phylogeny of the clade and how crop phenotypes and use cases have shifted over the course of human history, but I wouldn't do it here at the Quick-Stop.

So the main takeaway for the average grower is, do I fruit exotics on it or feed it to my horse?

I was speaking colloquially about how hay and straw are grown, labeled, and marketed. Farmers aren't turning up at the juice bar to see if they have any leftover wheatgrass because there's a shortage of wheat plants.

--------------------

ACAB 🖕🧊

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Dynasty
Stranger

Registered: 02/21/24
Posts: 259
Last seen: 2 months, 12 days

Re: Ask Quick Questions, Get Quick Answers

[Re: humble student]
#29332457 - 09/01/25 07:46 PM (1 year, 2 months ago)

humble student said:

After all of these plates were PCed, the lids never came off. They were moved to the SAB, unwrapped from the aluminum foil, and then cling-wrapped. They were ready to use, or, I should say, I thought they were ready to use.

Did you vent the pc?

--------------------
Beefs Hitchhikers guide
D3s agar tek

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Breadly710
Baker

Registered: 02/19/24
Posts: 582
Last seen: 14 days, 9 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: Dynasty]
#29332463 - 09/01/25 07:54 PM (1 year, 2 months ago)

Why does Bluey Vuitton hate me. I get them to germ, but they won’t clean up for me.

--------------------
Hitchhikers guide to the Shroomery

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BeefExtreme
Meatkeeper

Registered: 11/03/11
Posts: 14,589
Loc:

Last seen: 5 hours, 32 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Dynasty]
#29332464 - 09/01/25 07:54 PM (1 year, 2 months ago)

he did. id jump in to help but i dont know whats wrong. guess if it were me id just try something else. like pours for example

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BeefExtreme
Meatkeeper

Registered: 11/03/11
Posts: 14,589
Loc:

Last seen: 5 hours, 32 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Breadly710]
#29332465 - 09/01/25 07:56 PM (1 year, 2 months ago)

Breadly710 said:

Why does Bluey Vuitton hate me. I get them to germ, but they won’t clean up for me.

ive seen some ingenious cleanup teks. cabin sequestering, trench warfare, and dv8dug's condiment cup "ladder" tek which i think is the very best.

--------------------
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Breadly710
Baker

Registered: 02/19/24
Posts: 582
Last seen: 14 days, 9 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: BeefExtreme]
#29332475 - 09/01/25 08:00 PM (1 year, 2 months ago)

BeefSupremeJr said:

ive seen some ingenious cleanup teks. cabin sequestering, trench warfare, and dv8dug's condiment cup "ladder" tek which i think is the very best.

I am going to try some of those. It will look good, but if I put it to grain it contams. All the other jars from that session are looking good, so I think it’s the plates, but maybe I keep getting sloppy on BV jars.

--------------------
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BeefExtreme
Meatkeeper

Registered: 11/03/11
Posts: 14,589
Loc:

Last seen: 5 hours, 32 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Breadly710]
#29332477 - 09/01/25 08:01 PM (1 year, 2 months ago)

try dv8's trick. its in his signature i believe

--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion
✸-✸-✸
The Hitchhiker's Guide to the Shroomery

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LadysKnight
Hello Ladies

Registered: 10/09/15
Posts: 3,349
Loc: Between me and you

Re: Ask Quick Questions, Get Quick Answers

[Re: humble student]

3
#29332478 - 09/01/25 08:02 PM (1 year, 2 months ago)

humble student said:

I'm careful to turn off all HVAC systems, every window and door is closed, the SAB arm holes are covered with cling-wrap when not in use, I wear an N95 mask, gloves.

Bottom line is, it's something that I'm doing, or not doing.

Are you rapidly depressurizing the PC? Or do you let it depressurize and cool down on its own?

--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Extras:

callmehippie
☮️🍄Hallucinogenic Hippie🍄☮️

Registered: 05/28/24
Posts: 3,509
Loc: Over the hills and far away

Last seen: 2 hours, 57 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: boxtrotalpha]
#29332496 - 09/01/25 08:19 PM (1 year, 2 months ago)

boxtrotalpha said:

tinkscat said:

First grow - ziploc - M.Elf...

Are these ready to harvest? Or do the caps need to open up more?

Thanks team 🙏

Looks good to me homie. Those helmets usually don't open much. I take the wavy edges as my sign

a bunch of visible bruising on the stipes and stuff is also another good sign to harvest. when your unsure, and they've been around for a while

Also the soft marshmallow feel like dk had said.

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Take it easy. if it's easy. take it twice :mushroom2:

:mushroom2:

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tinkscat
Stranger

Registered: 04/05/25
Posts: 57
Loc: NZ

Last seen: 9 months, 30 days

Re: Ask Quick Questions, Get Quick Answers

[Re: boxtrotalpha]
#29332509 - 09/01/25 08:39 PM (1 year, 2 months ago)

boxtrotalpha said:

tinkscat said:

First grow - ziploc - M.Elf...

Are these ready to harvest? Or do the caps need to open up more?

Thanks team 🙏

Looks good to me homie. Those helmets usually don't open much. I take the wavy edges as my sign

Thanks for the tip!

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humble student
Stranger

Registered: 04/29/25
Posts: 73
Last seen: 10 months, 28 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Dynasty]
#29332513 - 09/01/25 08:46 PM (1 year, 2 months ago)

Yes, for 10 minutes after the air vent/cover lock lifted. My recollection from what I've read here at the shroomery is, vent for 10 minutes after the air vent/cover lock lifts, and PC for 20 minutes at 17psi.

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Dynasty
Stranger

Registered: 02/21/24
Posts: 259
Last seen: 2 months, 12 days

Re: Ask Quick Questions, Get Quick Answers

[Re: humble student]
#29332530 - 09/01/25 09:11 PM (1 year, 2 months ago)

humble student said:

Yes, for 10 minutes after the air vent/cover lock lifted. My recollection from what I've read here at the shroomery is, vent for 10 minutes after the air vent/cover lock lifts, and PC for 20 minutes at 17psi.

Yeah I don’t know. How do the 2 500 ml jars of agar look? If they haven’t contamed I would assume it has something to do with the No pour

--------------------
Beefs Hitchhikers guide
D3s agar tek

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humble student
Stranger

Registered: 04/29/25
Posts: 73
Last seen: 10 months, 28 days

Re: Ask Quick Questions, Get Quick Answers

[Re: LadysKnight]

1
#29332558 - 09/01/25 09:46 PM (1 year, 2 months ago)

Thank you, LadysKnight, for helping me with this.

"Are you rapidly depressurizing the PC? Or do you let it depressurize and cool down on its own?"
I follow the instructions in the booklet that came with the PC; I do not attempt to speed the cooling of the canner, and when the pressure has been completely reduced, and the air vent/cover lock has dropped, then I remove the pressure regulator, and let the PC cool overnight.

I'm a bit frustrated because I was having pretty good luck not having contamination the first 3-4 times I fixed and inoculated agar plates.

Thanks again for offering ideas and suggestions about what may be causing this contamination issue.

I won't give up; I will continue until successful :smile:

:smile:

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Proposition 122
Bonehead

Registered: 11/14/22
Posts: 715
Loc: Colorado

Last seen: 9 months, 16 days

Re: Ask Quick Questions, Get Quick Answers

[Re: humble student]
#29332568 - 09/01/25 09:56 PM (1 year, 2 months ago)

humble student said:

Yes, for 10 minutes after the air vent/cover lock lifted. My recollection from what I've read here at the shroomery is, vent for 10 minutes after the air vent/cover lock lifts, and PC for 20 minutes at 17psi.

Some of us vent for 20:00

--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

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Proposition 122
Bonehead

Registered: 11/14/22
Posts: 715
Loc: Colorado

Last seen: 9 months, 16 days

Re: Ask Quick Questions, Get Quick Answers

[Re: humble student]

1
#29332573 - 09/01/25 09:58 PM (1 year, 2 months ago)

humble student said:

<snip> ".... then I remove the pressure regulator..."

At this point you just compromised your sterility.

--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

Fruiting Cubes

Golden Teachers

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humble student
Stranger

Registered: 04/29/25
Posts: 73
Last seen: 10 months, 28 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Proposition 122]
#29332580 - 09/01/25 10:05 PM (1 year, 2 months ago)

That's a brilliant idea, Dynasty! Both 500mL media bottles containing agar that were PCed with the plates show no signs of contamination!

I believe Tryptkaloids and LadysKnight are correct; the contamination must be occurring when moving the plates from the PC to the SAB, and/or in my SAB procedures.

Thanks for your input.

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