Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   Original Sensible Seeds High THC Strains   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck

Jump to first unread post Pages: < First | | 3096 | 3097 | 3098 | 3099 | 3100 | 3101 | 3102 | 3103 | 3104 | 3105 | 3106 | 3107 | 3108 | 3109 | 3110 | 3111 | 3112 | 3113 | 3114 | 3115 | 3116 | | Last >
Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon]
    #29323099 -

lets stop talking about this and instead talk about things I care about.

is there a current link to something documenting best-known practices for cloning from dried fruit or do I just yolo it and crush up some mushrooms, soak them in distilled water, strain, put to agar, and worry about extracting some clean growth later to transfer to a new plate?

thank you in advance.

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29323104 -

Where did you hear that tric eats mycelium? Not claiming any special knowledge here but as I understand it tric out competes mycelium for the available food supply.


--------------------
“We either make ourselves miserable or we make ourselves strong. The amount of work is the same”

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: el53] * 1
    #29323106 -

nope.

Quote:
Causal agent of disease​​ Many species of Trichoderma are major pathogens of cultivated mushrooms



you can see it under a microscope.
https://www.researchgate.net/figure/Competition-of-Trichoderma-over-fungal-pathogen-in-co-culture-plate-Parasitism-The_fig1_344224561

pic link wont work.

Edited by mushboy (08/23/25 01:28 PM)

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: FuzzyFoot]
    #29323116 -

FuzzyFoot said:
el53 said:
So at 20 psi you sacrifice nutrition in your grain f aor time required at correct sterilization temperatures. It really isn't my opinion it's every food canning standard that says that.




Where? Please show us all proof of this. Please provide the studies that you read that back this up in any way. That 20 psi will degrade the nutritional viability of the grains used in mushroom cultivation in a measurable way. Or STFU.





Literally took 20 seconds to find. I am not making this up. You want to do it a new way that other science shows is problematic? Show before and after nutritional studies.
https://www.sciencedirect.com/science/article/abs/pii/S0733521021000400


--------------------
“We either make ourselves miserable or we make ourselves strong. The amount of work is the same”

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: el53] * 1
    #29323117 -

Any chance we can get the LaTeX markup add on added to the forum software so we can display complex equations? I’m not sure the best place to ask so if I can just be pointed to the proper place to ask that would be great.


--------------------
You can ignore the truth to your final breath. But trust me, it will meet you there to be ignored no longer.

The answer to your question is almost always 42
“The answer is always grow more shroom.” Mr Piggy
- Those two answers coexist peacefully.

Quick Sterilization Reference for Altitude

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: 1NFIN8]
    #29323130 -

The dogs here are not interested in science, el53.

They just enjoy picking on those who are.

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: anders]
    #29323150 -

why is anyone arguing about this?  of course trich eats mycelium, what the damn else is there to eat?  coir doesn't catch trich, it literally has to be mixed into it coir for gardening purposes.

and stop stealing my title you fraud (anders).

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: dinosaurs] * 1
    #29323153 -

i dont know.
:ohshitno:

although hes only temp banned for 2days not perm so the title could use an update.

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: anders]
    #29323173 -

Sadly obvious


--------------------
“We either make ourselves miserable or we make ourselves strong. The amount of work is the same”

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: mushboy] * 3
    #29323174 -

Trichoderma is a genus of fungi that is well-known for being mycoparasitic — meaning it can parasitize and feed on other fungi, including their mycelium. It is often seen as a contaminant in mushroom cultivation because it aggressively invades and degrades the mycelium of other mushrooms.

Trichoderma secretes enzymes (like chitinases and glucanases) that break down the cell walls of other fungi. Then it absorbs the nutrients from the digested mycelium.


--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

Fruiting Cubes


Golden Teachers

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122] * 1
    #29323177 -

What is the "F" designation in some of the mushroom names? I have seen F2 and F5. I don't remember the species name it was attached to but the format was, for example, "Golden Teacher F2".


--------------------



Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Gilmour Hendrix] * 3
    #29323179 -

Gilmour Hendrix said:
What is the "F" designation in some of the mushroom names? I have seen F2 and F5. I don't remember the species name it was attached to but the format was, for example, "Golden Teacher F2".



Filial. It designates what generation it is.

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns] * 1
    #29323183 -

Thanks! A new generation starts from spore or clone?


--------------------



Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Gilmour Hendrix] * 1
    #29323190 -

Gilmour Hendrix said:
Thanks! A new generation starts from spore or clone?



Spore.

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns] * 2
    #29323198 -

Bear in mind this is breeding terms and only applies to crossing.

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: the_chosen_one]
    #29323207 -


I’m looking for a 16 qt tub and the target brand one has ridges that im not sure about, should i get the 30 qt? its the save length/width just deeper


--------------------

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: toadking]
    #29323211 -

I'm not sure if you're close to home Depot or Menards but they carry EZ Storage and the sides don't go in nearly as much. Not sure if they have 16 qt tho

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: dinosaurs]
    #29323213 -

Asking for an information source is suddenly arguing? As I understand it tric faster and more absorbs grain than the mycelium thus starving the mycelium. Todate I've never seen any evidence of it eating mycelium.I have seen it break the barrier of colonization to steal grain none of which would be an issue if you ran high pH tubs.


--------------------
“We either make ourselves miserable or we make ourselves strong. The amount of work is the same”

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns] * 1
    #29323215 -

RoscoeReturns said:
el53 said:
Let me try to put this a different way and give you an example to prove it. The whole point of pressure is to get into the temperature range of 250 to 260 for boiling water also this thing that it creates for sterilization. If you go too high you can literally with pressure turn your grain into sludge and if not all of it certainly that accounts for all that weird moisture at the bottom of jars and I can prove this. Ever tried pressure cooking a turkey leg? They really come out awesome just look up the right PSI for where you live throw a steamer basket and a turkey leg in but while you're at it add an onion. 10-15 minutes after you reach pressure turn off your heat, let the pressure cooker naturally aspirate and open. Your turkey leg is fall off the bone tender but where's your onion it's disappeared  and you magically have onion gravy under your steamer rack. Why you may ask. Because the onion did not require high pressure cooking and was simply pressured into delicious sludge. Sterilizing your grains at over psi's will do the same thing to them. Don't ever ask yourself again why you have sludge in the bottom of a bag or a jar.



If your grain is properly hydrated it will not turn to sludge. I’ve run much higher pressures with no problem.


mushboy said:
ive sterilized grains at 25psi they do not turn to mush you are making things up my man.



:whathesaid:





It's crazy cuz I agree with you guys and not him. Because I cook grains at a high pressure all the time and nothing mushy happens. However if you do any searches on Google what's the required PSI for pressure canners at sea level it does say

At sea level (0-1,000 feet), a weighted-gauge pressure canner should operate at 10 psi, while a dial-gauge canner should be set to 11 psi to reach the necessary 240°F internal temperature for safe food preservation. The difference is due to the pressure needed to overcome the atmospheric pressure and the gauge's potential for slight inaccuracy, with 11 psi being easier to monitor on a dial.



And I'm like how have I never seen this before. I guess I've never searched those exact specific words.

Also in Presto's manual and every other manual I've seen about pressure canning. It's specifically States pressure can at 15 psi. But it doesn't say anything about what sea level you're at unless you're at a higher elevation it says to go higher or for longer time.


To be clear I still say even though that's what Google says and everything. I always cook my grains at around 19 PSI for 2 and 1/2 to 3 hours and never get any mushy grains or anything.

:shrug:


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 3
    #29323223 -

From another longer post that I am researching and putting together:

We aren't canning. We are sterilizing. The majority of info out there is canning focused. This also means that the time suggestions for altitude differences are completely wrong when applied to sterilization.

A few reasons why mycology sterilization is different and more important than canning:
  • Incubation Conditions Demand Absolute Sterility: In mycology, sterilized grains are inoculated with mycelium and incubated at room temperature (typically 20–30°C) for 1–4 weeks, creating ideal conditions for any surviving microbes or spores to germinate and outcompete the slow-growing fungi. Canning aims for commercial sterility where sealed jars prevent growth during ambient storage, allowing for potentially lower kill thresholds without immediate contamination risks.
  • Broader Spectrum of Contaminants: Mycology must eliminate not only bacterial endospores (like Clostridium botulinum in canning) but also a wider array of fast-growing molds (e.g., Trichoderma), yeasts, and airborne bacteria that can enter via filter patches, inoculation, poor SAB work, and a myriad of other ways. Canning focuses primarily on botulism and spoilage organisms in a sealed environment, with metrics tailored to those specific threats.
  • Heat Penetration and Process Times: Grain substrates in mycology are dense and porous, requiring longer pressure cooking times (90–120 minutes at 15 psi) to ensure uniform heat distribution and penetration to the core, often exceeding standard canning guidelines (e.g., USDA times of 20–90 minutes for low-acid foods) due to slower thermal transfer and the need for overkill to account for variable home setups.
  • Substrate Integrity and Nutrient Concerns: Over-processing in mycology can cause grains to burst or degrade nutrients (e.g., starch gelatinization affecting mycelium colonization), necessitating balanced metrics not emphasized in canning, where the focus is on food safety and texture rather than supporting live fungal growth.
  • Recontamination Risks: Canning relies on vacuum sealing to create an anaerobic, contamination-proof environment post-processing. Mycology uses breathable lids or bags for gas exchange during colonization, increasing vulnerability to external contaminants and thus requiring more robust initial sterilization beyond typical canning protocols.


--------------------
You can ignore the truth to your final breath. But trust me, it will meet you there to be ignored no longer.

The answer to your question is almost always 42
“The answer is always grow more shroom.” Mr Piggy
- Those two answers coexist peacefully.

Quick Sterilization Reference for Altitude

Extras: Filter Print Post Top
Jump to top Pages: < First | | 3096 | 3097 | 3098 | 3099 | 3100 | 3101 | 3102 | 3103 | 3104 | 3105 | 3106 | 3107 | 3108 | 3109 | 3110 | 3111 | 3112 | 3113 | 3114 | 3115 | 3116 | | Last >

Shop: Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   Original Sensible Seeds High THC Strains   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck


Similar ThreadsPosterViewsRepliesLast post
* quick question, finished colonizing? usefulidiot 1,861 1 04/06/03 06:31 PM
by kindkesey
* Quick Question grateful_head 1,240 2 08/18/01 03:40 PM
by Billyblastoff
* New to the boards, and a quick question to start ! Anonymous 1,846 4 03/11/02 03:18 AM
by 2shrewmen
* some quick questions about my treasure coasts alf 3,858 19 11/16/01 01:54 AM
by Anonymous
* Quick Question needs quick answer Wheedhead! 1,186 2 01/19/03 09:06 AM
by Wheedhead!
* quick question SuppaFly 1,044 1 09/18/02 04:41 PM
by mickey_rourke
* Re: Quick Question
( 1 2 all )
Anonymous 4,549 22 06/14/00 05:46 AM
by hitterg
* Quick Question LXTC 1,808 11 09/24/02 10:09 PM
by LXTC

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: DERRAYLD, Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,186,131 topic views. 8 members, 739 guests and 83 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.073 seconds spending 0.048 seconds on 16 queries.