PhenoDreamers said:

Puffin said:

I did have another idea about isolating monokaryons which is to filter the spore solution through a 20 μm filter prior to cell counting and dilution with the aim of eliminating the majority of the clumps and increasing the single spore ratio.

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Are you using polysorbate?

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anders said:

UV is a very positive step yes, but it is not what I mean nor said up to this point.

Why would I contribute new science, when I get the above?

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PhenoDreamers said:

But you're doing this to isolate monokaryons and that's it? Just do early t1s from your serial dilution germ plate dude. You're over thinking it. People isolate monos all the time even off streaked plates. Just have to get your timing right.

Also, what do you plan on doing with subaeruginosa and natalensis monos?

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anders said:

IOW, you're not really going to learn much from this Puffin.  You'll get several puffballs wadded together, and intermingling myc.

It is adviseable to spread out genetics so you can choose the most promising and isolate the nasties.

But, we all learn our own ways.

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PhenoDreamers said:

Let's leave h2o2 out of this. I've at least proven it's definitely effective. It's certainly better than iso. However, people always want to use it in manners that are impractical. I don't use iso at all. Only h2o2.

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I think its important to note what you are using it for.

When i got here people were largely spraying tubs with it to fight off mold.

That - i dont suggest.

As. Sanitizer? Sure. i assume the common store bought stuff at 4% isnt enough?

BeefSupremeJr said:

pretty sure i put UV, ozone, and h202 in the debunked obsolete thread.  these things do more harm than good. 

uvb and uvc arent used in labs becuse they degrade plastics and could even cause irreparable skin damage.  as someone who is struggling with skin cancer, i dont want it near me.

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tryptkaloids said:

PhenoDreamers said:

Let's leave h2o2 out of this. I've at least proven it's definitely effective. It's certainly better than iso. However, people always want to use it in manners that are impractical. I don't use iso at all. Only h2o2.

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I think its important to note what you are using it for.

When i got here people were largely spraying tubs with it to fight off mold.

That - i dont suggest.

As. Sanitizer? Sure. i assume the common store bought stuff at 4% isnt enough?

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mushboy said:

this is a thread about agar. not your stomping ground to soapbox about uv lights or whatever.

make your own thread.

record broken or what?

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anders said:

This is all I have to say.

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anders said:

There is just no debating the effectiveness of UV-C as a followup to conventional cleaning, in particular WRT airborne particulates.  Does this hobby not have this problem?  Yes or No?

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anders said:

mushboy said:

this is a thread about agar. not your stomping ground to soapbox about uv lights or whatever.

make your own thread.

record broken or what?

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See?  And yet lately this thread has been personal attacks on me.

UV has everything to do with agar work, if you think about it.

Don't like it because I advocate?  Don't use it. 

This is all I have to say.

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anders said:

mushboy said:

this is a thread about agar. not your stomping ground to soapbox about uv lights or whatever.

make your own thread.

record broken or what?

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See?  And yet lately this thread has been personal attacks on me.

UV has everything to do with agar work, if you think about it.

Don't like it because I advocate?  Don't use it. 

This is all I have to say.

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Most of us here can show better plates grown without uv than you have shown with it.

Im gonna pour a stack of plates in and leave in a filthy sab left open, plates unwrapped. Imma grow on em after a week or 2.

Pour some plates, seal em up. Use em.

Lets see some clean plates made with these fancy, dangerous lights.

Otherwise its literally entirely talk.

Pic tax

Puffin said:

tryptkaloids said:

PhenoDreamers said:

Let's leave h2o2 out of this. I've at least proven it's definitely effective. It's certainly better than iso. However, people always want to use it in manners that are impractical. I don't use iso at all. Only h2o2.

---

I think its important to note what you are using it for.

When i got here people were largely spraying tubs with it to fight off mold.

That - i dont suggest.

As. Sanitizer? Sure. i assume the common store bought stuff at 4% isnt enough?

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From my basic reading, 7.5%+ is the minimum for it to be sporicidal/sterilizing and requires a contact period of 6+ hours. 12% is available on Amazon.

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anders said:

mushboy said:

this is a thread about agar. not your stomping ground to soapbox about uv lights or whatever.

make your own thread.

record broken or what?

---

See?  And yet lately this thread has been personal attacks on me.

UV has everything to do with agar work, if you think about it.

Don't like it because I advocate?  Don't use it. 

This is all I have to say.

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