LotKid said:

And why does it need a roof?

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LotKid said:

And why does it need a roof?

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Scemilope said:

LotKid said:

And why does it need a roof?

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To make 'em work for it. 😂

Mostly it's a tool used to hinder bacteria but can  also be used for selecting strong fast moving myc.
It's basically an obstacle course that our chosen are better at overcoming than our bacterial rivals are. Bacteria has low motility out of water, our mycelium is better at crossing the empty gap and fighting it's way out of the sequester.
An agar roof, no roof/trenching also works well. Doesn't help as much with molds unfortunately.

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LotKid said:

Scemilope said:

LotKid said:

And why does it need a roof?

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To make 'em work for it. 😂

Mostly it's a tool used to hinder bacteria but can  also be used for selecting strong fast moving myc.
It's basically an obstacle course that our chosen are better at overcoming than our bacterial rivals are. Bacteria has low motility out of water, our mycelium is better at crossing the empty gap and fighting it's way out of the sequester.
An agar roof, no roof/trenching also works well. Doesn't help as much with molds unfortunately.

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well, i guess if you have no other options or backups and need to save a culture that's worth it's weight in gold.... cool

tryptkaloids said:

LotKid said:

And why does it need a roof?

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Remember ye olde sandwich tek?

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yeah, i smelled a reinvention of the wheel at play

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anders said:

I want shrooms before I die.

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anders said:

Yep, I have excised all the green out of the plates (right after these pics), and it should clear up alot.

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anders said:

I don't take no advice...  {snif}

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anders said:

Whatever this cloud is that is submarining my agar, I do not think cabining will stop it.  I never would have seen it, if it weren't for my clear 1%.

Two of my cultures are just about ready for grain.  They were not affected by the bacterial cloud.

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anders said:

anders said:

Whatever this cloud is that is submarining my agar, I do not think cabining will stop it.  I never would have seen it, if it weren't for my clear 1%.

Two of my cultures are just about ready for grain.  They were not affected by the bacterial cloud.

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You know?  It is looking like this 'cloud' is just normal gelling of the MEA.  My control plate has it now equal to the others, with no sign of any contamination.  This is the first time I've done 1% so do not know what to look for.  I suspect I panicked too soon.  This is normal for 1% MEA.

But def excising all green is a good move, at least in my case where I use 4 quadrants.  Things are going very well now.

My PC is from my experiments way back, and now it is actually sealing after I treated the gasket with walnut oil.  It is practically reaching 2 bar!  I can not believe it.

In my struggles to get it up to pressure I did more than 5 cooks of this jarred grain, and ended up burning the shit out of it.  All trash.  I do though have another 4 quarts which I'm PCing now and another batch in soak for cooking.  I will strain off the soak water and sterilize that for hydration of sub.  Why?  Why not?

I am very interested in the results of the Rev's new grain when he gets them in.

And whenever will sleepydave return?

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anders said:

Quote:

anders said:.  I will strain off the soak water and sterilize that for hydration of sub.  Why?  Why not?

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Because grain water, and other nutritius subs will mold unless colonized in a sterile environment.