tryptkaloids said:

Arkyhog said:

I utilize the PP5 bead containers and typically allow them to cool overnight in either the pressure cooker or the instant pot. The following day, I simply wipe off any condensation from the lids with a paper towel in front of my ffu. While I often use them the day after running them, I also have some that I allow to sit for weeks before putting them to use.

Freshly poured before the IP run.

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That sounds terrible. Do you pc those with the lid on?

No pours have less condensation if allowed to cool completely before lidding and pcing.  With pouring, let it cool more.

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1NFIN8 said:

I’m curious. Has anyone used grain soak water in Milkboys Agar recipe? Would anything be gained? I would probably skip the molasses addition if I did it.

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Arkyhog said:

tryptkaloids said:

Arkyhog said:

I utilize the PP5 bead containers and typically allow them to cool overnight in either the pressure cooker or the instant pot. The following day, I simply wipe off any condensation from the lids with a paper towel in front of my ffu. While I often use them the day after running them, I also have some that I allow to sit for weeks before putting them to use.

Freshly poured before the IP run.

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That sounds terrible. Do you pc those with the lid on?

No pours have less condensation if allowed to cool completely before lidding and pcing.  With pouring, let it cool more.

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LoL, yes I PC them with the lids on, Once they cool I take them back to my grow tent in front of the ffu and remove the lids one at a time in front of the ffu and wipe any condensation out of the lids. I will take a few pictures in a minute to demonstrate the process.

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Arkyhog said:

tryptkaloids said:

Arkyhog said:

I utilize the PP5 bead containers and typically allow them to cool overnight in either the pressure cooker or the instant pot. The following day, I simply wipe off any condensation from the lids with a paper towel in front of my ffu. While I often use them the day after running them, I also have some that I allow to sit for weeks before putting them to use.

Freshly poured before the IP run.

---

That sounds terrible. Do you pc those with the lid on?

No pours have less condensation if allowed to cool completely before lidding and pcing.  With pouring, let it cool more.

---

LoL, yes I PC them with the lids on, Once they cool I take them back to my grow tent in front of the ffu and remove the lids one at a time in front of the ffu and wipe any condensation out of the lids. I will take a few pictures in a minute to demonstrate the process.

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Thisisfine said:

Well, I am a newbie myself, but for me it helps if I put a jar with hot wáter on the first plate of each stack and let it cool with the plates.
Hope it helps.

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Synack said:

OK, since I broke my question-asking cherry:
This is a plate I dribbled vendor LC on 6 days ago. Sorry about the image quality, the aforementioned condensation problem makes for cruddy pictures and lighting. But all I see are little white spots of mycelium, nothing resembling contamination.

How long do I let this initial plate grow out before making transfers? My plates are incubating at room temp, which is ~70F, which might slow growth a bit...

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boxtrotalpha said:

Depending on your knife skills you could get a few transfers of the leading edge of the big growth at 6oclock right now if you wanted.

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My knife skills are best described as "entirely untested." This will be my first transfer ever. But my general demeanor about these things is "challenge accepted!"

999999999 said:

in the beginning it's a numbers game,
take a bunch of them and get that practice going; fail more, fail better

(experiment transfers with a syringe needle, less is more, you just need one cell of mycelium - you don't need to see it, just need to touch it)

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Synack said:

boxtrotalpha said:

Depending on your knife skills you could get a few transfers of the leading edge of the big growth at 6oclock right now if you wanted.

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My knife skills are best described as "entirely untested." This will be my first transfer ever. But my general demeanor about these things is "challenge accepted!"

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Slow, smooth, steady movements.  Each action with purpose and intent.  Imagine you are standing shoulders deep in a swimming pool. How slow would your movements be?

Nothing hovers above an open petri other than your scalpel. Keep hands and fingers to the sides of plates at all times.

Take more transfers than you need.  I have been liking to put 3 transfers to each fresh petri. (Big thanks to Tree Frog for that suggestion)

You got this.