ThurmanMurman said:

Can I get a health check? Fully colonized 7/20.

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ThurmanMurman said:

Can I get a health check? Fully colonized 7/20.

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chillshroom said:

Damn, I wonder what I'm doing wrong to get so much bacterial contam. I had 5 go moldy before this, too. Before trying jars I did 19 instant rice bags and 17 were successful, and the culture doesn't look bacterial on agar that I can tell, so maybe it's the filters or the way I set up the jars and sealed the injection port. Or maybe I should sterilize at higher pressure or for longer--what's the risk of going too hot for too long, does it leave telltale signs?

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noomer said:

Any tips on germinating from swabs?

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LyleChipperson said:

Post some photos of your test agar plates. Also do you vent your PC before you start the sterilization timer?

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I do, 15 minutes to vent and only starting the timer when the steam plume reaches a full thick nearly solid-looking state and it's a good loud hiss. I do keep the gauge right on 15psi though and maybe my gauge isn't accurate. (I can't rely on the weight, I could only get a 12psi one for my model so I added coins until it can get to 17psi per the gauge.) Think I should try it at 17psi, or 15psi for longer? Any harm in that?

Here's my agar. Apologies for the awful pics, I'm using little jars no-pour style, tricky to get a clear shot.






It all looks white, the yellow tint is the lighting and the agar itself being a fading green. I know nothing about looking at agar so I shouldn't have said it looks non-bacterial, all I know is that it's got fluffy white. My agar technique is bad too, in that some of the jars came out runny and I squirted too much LC due to bad syringe handling and a bit of clogging. What do you think?

chillshroom said:

I do keep the gauge right on 15psi though and maybe my gauge isn't accurate. (I can't rely on the weight, I could only get a 12psi one for my model so I added coins until it can get to 17psi per the gauge.)

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ThurmanMurman said:

Can I get a health check? Fully colonized 7/20.

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KlarnEvil9 said:

I would love to find one of those that would fit Holy Grails, they are quite a bit smaller than petris.

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Quote:

IIRC, I think some time needs to be added to the sterilization cycle when using a container like that. The HG thread had notes on using a tall cookie tin or mason jar, maybe the extra time lines up.

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Quote:

Sounds a bit dicey if you aren't sure of the accuracy of the gauge. I would run the regulator unmodified and verify that the gauge reads 12psi first before adding the coins.

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AZ Starman2112 said:

ThurmanMurman said:

Can I get a health check? Fully colonized 7/20.

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Looks good to me! How fast did it recover after the last shake?

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KlarnEvil9 said:

I would love to find one of those that would fit Holy Grails, they are quite a bit smaller than petris.

IIRC, I think some time needs to be added to the sterilization cycle when using a container like that. The HG thread had notes on using a tall cookie tin or mason jar, maybe the extra time lines up.

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I have seen smaller ones.  I don't know if they're too small or not. But when I was looking around on Amazon last night all I could find was smaller ones that were made for sterilizing pipettes. When I was looking at it. was 62 mm diameter.



There's also a uk-based website I believe that has them that's selling them from 60 mm all the way up to 105 mm.



https://www.laborxing.com/products/sterilising-containers-for-petri-dishes-with-diameter-60-mm-to-105-mm?variant=42044465250521&country=DE¤cy=EUR&utm_medium=product_sync&utm_source=google&utm_content=sag_organic&utm_campaign=sag_organic&utm_campaign=gs-2021-07-21&utm_source=google&utm_medium=smart_campaign&gad_source=1&gad_campaignid=17519876816&gbraid=0AAAAAC8S2I5p1GBPmVuRzPf6JNRHC1f6G&gclid=Cj0KCQjws4fEBhD-ARIsACC3d29figDyWgcbbB-gxbwkyrG9vrOzs7NpylHR_W5DnVLcBl_khtp4XlIaAhUmEALw_wcB


This looks like an American site.



https://labxsci.com/product/sterilising-containers-for-petri-dishes-with-diameter-60-mm-to-105-mm/?attribute_variant=%CE%A675x180+mm+for+%CE%A660+mm+Petri+dish&gad_source=1&gad_campaignid=22231892628&gbraid=0AAAAA9-hapMqfGvu60F7DCa-LHRlYwVtr&gclid=Cj0KCQjws4fEBhD-ARIsACC3d28lQEEmIN3lPbK872ohS0I4gaXxpe4sNw3fpV8FKrt0IErj2rwzVxYaAucuEALw_wcB

They have 60mm 75mm and 90mm.

callmehippie said:

KlarnEvil9 said:

I would love to find one of those that would fit Holy Grails, they are quite a bit smaller than petris.

IIRC, I think some time needs to be added to the sterilization cycle when using a container like that. The HG thread had notes on using a tall cookie tin or mason jar, maybe the extra time lines up.

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I have seen smaller ones.  I don't know if they're too small or not. But when I was looking around on Amazon last night all I could find was smaller ones that were made for sterilizing pipettes. When I was looking at it. was 62 mm diameter.



There's also a uk-based website I believe that has them that's selling them from 60 mm all the way up to 105 mm.



https://www.laborxing.com/products/sterilising-containers-for-petri-dishes-with-diameter-60-mm-to-105-mm?variant=42044465250521&country=DE¤cy=EUR&utm_medium=product_sync&utm_source=google&utm_content=sag_organic&utm_campaign=sag_organic&utm_campaign=gs-2021-07-21&utm_source=google&utm_medium=smart_campaign&gad_source=1&gad_campaignid=17519876816&gbraid=0AAAAAC8S2I5p1GBPmVuRzPf6JNRHC1f6G&gclid=Cj0KCQjws4fEBhD-ARIsACC3d29figDyWgcbbB-gxbwkyrG9vrOzs7NpylHR_W5DnVLcBl_khtp4XlIaAhUmEALw_wcB


This looks like an American site.



https://labxsci.com/product/sterilising-containers-for-petri-dishes-with-diameter-60-mm-to-105-mm/?attribute_variant=%CE%A675x180+mm+for+%CE%A660+mm+Petri+dish&gad_source=1&gad_campaignid=22231892628&gbraid=0AAAAA9-hapMqfGvu60F7DCa-LHRlYwVtr&gclid=Cj0KCQjws4fEBhD-ARIsACC3d28lQEEmIN3lPbK872ohS0I4gaXxpe4sNw3fpV8FKrt0IErj2rwzVxYaAucuEALw_wcB

They have 60mm 75mm and 90mm.

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I wonder, which is true?

ThurmanMurman said:

AZ Starman2112 said:

ThurmanMurman said:

Can I get a health check? Fully colonized 7/20.

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Looks good to me! How fast did it recover after the last shake?

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At 24hrs, it recovered well enough to remain solid when I flipped the jar.

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tree frog said:

I took nine samples to three 90mm plates for the first cluster.  And again for the second.

Six plates total.  All water agar.

I cabin sequestered the best six on 0.5% lme.  Two more plates.



I took samples from the six cabins to six 0.5% lme plates for masters and six half pint jars.  One jar went green.  I took the label from this jar and put it on the master plate for reference.  And one master showed some discoloration so I'll keep a close eye on the tray on that clone.

14 plates.  Six small jars.  Five small trays made it to fruiting.



That was eight days ago, still waiting on pins but bisporus can take a bit.  All samples from these two clusters.




Anyway, sorry that's not a quick answer the butter snuck up on me.  but yeah, it's worth it.  A good culture I can fruit for the rest of my life if I take good care of it.  And these mushrooms in particular have given me a lot, so I enjoy taking care of them.

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