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Re: Ask Quick Questions, Get Quick Answers [Re: boxtrotalpha]
    #29292323 -

It may, let ‘er ride and see what ya get


--------------------
Beef’s The Hitchikers Guide to Shroomery
Stipe’s Clean Spawn Checklist
Gummies by milkboy
Eat’s Easy Oats Prep
DV8DUG’s Recognizing Contamination in Grain Jars

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Re: Ask Quick Questions, Get Quick Answers [Re: Headass]
    #29292325 -

I dont see any green


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29292344 -

I see heavy bruising along the entire top of the tub with a dead zone of coir between it and the healthy myc.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29292345 -

tryptkaloids said:
I dont see any green


My phone camera sucks and you'll have to zoom in



Pretty sure the bruising up top is just a shadow from my printer though


--------------------
Where to start?

Are your plates clean?


Edited by boxtrotalpha (07/23/25 04:04 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: boxtrotalpha]
    #29292348 -

Oh, I missed that.  Is what I’m seeing at the top trick of light or is something going on there?


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: boxtrotalpha]
    #29292351 -

Or I could just take better pictures, featuring green and former top side lol



The myc that was on top is definitely weaker looking growth than the rest piggy but mostly trick of light


--------------------
Where to start?

Are your plates clean?


Edited by boxtrotalpha (07/23/25 04:10 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: boxtrotalpha] * 1
    #29292355 -

I mean you know what I’m going to say at this point.
:sunny:


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] * 1
    #29292356 -

:sad: yeeeaaaaaahhhhh

Still letting it ride away from the other though
:fuckthepolice:


--------------------
Where to start?

Are your plates clean?


Edited by boxtrotalpha (07/23/25 04:13 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29292361 -

ThurmanMurman said:
fahtster said:
Ihe said dry grains.  Put em in a swab pack.  They’ll last for years. Cryovials work too.. I’d just be sure to wrap em




I just got cryovials and as I held them in my hand, I realized that I dont have any kind of plan for how to stand this tiny vial up with the cap off, place sample, and get the cap back on. All without spilling it or contaminating it. 🤣

Grains might be the way - thanks for mentioning it.🤙




They do make stands for them. I've got a couple. I've got some for the cryovals. And some for the mini eppies/Micro Centrifuge tubes. The little plastic cone shaped ones.






Vial Rack, HPLC Sample Bottles Rack https://a.co/d/aCtA2zYMicrocentrifuge

Tube Holder https://a.co/d/5IWWrPD


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: boxtrotalpha]
    #29292363 -

boxtrotalpha said:
:sad: yeeeaaaaaahhhhh

Still letting it ride away from the other though
:fuckthepolice:


Maybe big mutant coming out of it and youre able to clone the genetics for the rest of us.

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Re: Ask Quick Questions, Get Quick Answers [Re: ogelite]
    #29292365 -

ogelite said:
boxtrotalpha said:
:sad: yeeeaaaaaahhhhh

Still letting it ride away from the other though
:fuckthepolice:


Maybe big mutant coming out of it and youre able to clone the genetics for the rest of us.


If nothing else that tiny pin that's already popped up is a gahd damn warrior. Gonna make sure it's legacy live on lol


--------------------
Where to start?

Are your plates clean?


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Re: Ask Quick Questions, Get Quick Answers [Re: boxtrotalpha]
    #29292368 -

boxtrotalpha said:
ogelite said:
boxtrotalpha said:
:sad: yeeeaaaaaahhhhh

Still letting it ride away from the other though
:fuckthepolice:


Maybe big mutant coming out of it and youre able to clone the genetics for the rest of us.


If nothing else that tiny pin that's already popped up is a gahd damn warrior. Gonna make sure it's legacy live on lol


Gotta farm some mold man!

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Re: Ask Quick Questions, Get Quick Answers [Re: fahtster]
    #29292373 -

fahtster said:
chris77 said:
how to do this without a hood? in the sab?


I make specialized lids Here but like I said, you can just leave some grains in the bottom of your jar when you spawn.. it takes a while because most spawn jars only have small ge filters.  One could probably pc some tyvek pouches and put em in there in your SAB.  I’d probably double up on the tyvek tho



This might sound dumb or seem obvious. Because I know the lids have to be sterile.

But

I've been wondering about this for a while. When you make these lids. Are they used from the very beginning? Meaning do you put  uninoculated grain in the jar and then make these lids and use these Lids to do the PC process and inoculation.  And that its it?

They fully colonize and then dry out? Or is there some point where the lids are switched out from a normal lid to your special drying lid? I've been wanting to do this but I'm unsure about the actual process. other than actually  making the lid  Is there any way you could explain the actual process of drying  grains while keeping sterility. 

I know you use tiny jelly jars and put them in front of a fan and everything but I'm just unsure about how or when the lid gets put to the jar..

Sorry if I'm repeating myself. Please explain the actual whole Tek from start to finish  "how i dry my myc grain" and not just the lids if you don't mind.

I do have a couple spawn jars that just have the last tiny little bit of grains left in the bottoms. But I'm really wanting to give your way a go.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29292383 -

I’m not the master, but when I dry grain I have two options.

1. If I use two piece lids with 1/4” hole in the metal lid - I use a full size SFD from the beginning over the metal lid and just switch to a ring only when I need to dry.

Or

2. If I used my one piece filtered lids - Sterilize some SFD’s wrapped in foil pouches. I’ll swap this on in my FFU or SAB and retain with a ring.


--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Ask Quick Questions, Get Quick Answers [Re: boxtrotalpha]
    #29292415 -

boxtrotalpha said:


So my question, do I let it ride as is and see what I get or do I try to extract the green and surrounding area?


I let it ride.

Because some colonies can stave off green for awhile, and cutting up the substrate is stressful for them and work for me.

This is a pan but they had a spot of green before I had pins for the first flush.  This photo is the second flush.



That said, green can be a lot of different species of mold. And the one I had wasn't very aggressive.

Edit:  stoned and didn't see that the thread had moved on lol


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (07/23/25 05:49 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29292432 -

tree frog said:
koma23 said:
tree frog said:
If you want bigger fruits, clone single fruits from the first flush rather than clusters.

It's still not a guarantee.  Because it's not like cloning plants, there will still be a lot of genetic variability in a single mushroom.

Unless you're starting from an isolate.  But then there's no reason to clone.


Ja, cloning singles hasn't worked that great for me, but I see your logic tnx.


Yeah, it's not generally recommended.  Cluster clones tend to give better coverage and yield.

The lack of guarantee is why I take multiples from grows I like.

Gnats got this tray unfortunately.  But I took eighteen samples between this cluster and another one.  The best six of those were cabin sequestered and taken to grain.  One of the six jars went green.  The other five are in fruiting.

My two favorites will go into long term storage.



A good balance of speed, good fruit size and morphology, and cluster density.  That's what I prefer in clone candidates.


Wait, are you saying you made 18 clone samples, ie. 18 plates from this one grow!?

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Re: Ask Quick Questions, Get Quick Answers [Re: koma23] * 1
    #29292447 -

Thats exactly what shes saying.

I imagine pans are a lot tougher to clone


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: koma23] * 2
    #29292460 -

I took nine samples to three 90mm plates for the first cluster.  And again for the second.

Six plates total.  All water agar.

I cabin sequestered the best six on 0.5% lme.  Two more plates.



I took samples from the six cabins to six 0.5% lme plates for masters and six half pint jars.  One jar went green.  I took the label from this jar and put it on the master plate for reference.  And one master showed some discoloration so I'll keep a close eye on the tray on that clone.

14 plates.  Six small jars.  Five small trays made it to fruiting.



That was eight days ago, still waiting on pins but bisporus can take a bit.  All samples from these two clusters.




Anyway, sorry that's not a quick answer the butter snuck up on me.  but yeah, it's worth it.  A good culture I can fruit for the rest of my life if I take good care of it.  And these mushrooms in particular have given me a lot, so I enjoy taking care of them.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (07/23/25 06:23 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29292480 -

Also, I should mention it's hard to get clean samples with pans.  They are little.

If it was something easier to clone I would do three samples to one plate in the beginning rather than nine to three.

But essentially I like to fruit three clones per cluster.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29292521 -

I bought a 5 pack of rapid rhizo agar plates from Midwest grow kits and swabbed two plates on the 17th. There’s absolutely no growth of any kind. Should I see anything by now? I’m new to agar


--------------------
~Wherever you go in life, there you’ll be~




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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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