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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #29255184 -

Cheers :smile:

So just to be sure.

With bod agar tek : https://www.shroomery.org/forums/showflat.php/Number/21922023

and either pk tek or puck tek https://www.shroomery.org/forums/showflat.php/Number/28649340/fpart/all

1. I'm all set and don't need more tools than what they list?

2. if i've understood everything correctly : agar following bod tek, then transfer to another plate until clean culture, then transfer to puck / cake / monotub. That's all? No need to follow another tek?

3. Which tek to follow for agar to agar transfer tek? And which guide to read about it? (when, what to look for, what to not look for, ...)

Cheers everyone, promise i'll stop soon with my questions :smile:


--------------------
wow

Edited by Tropical (06/19/25 05:51 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Fauchinger] * 3
    #29255188 -

Fauchinger said:
This is a professional setup that I can only dream of.
I wipe down my desk with iso, spray my SAB with soapy water (sometimes not even that), pour plates in there and hope for the best. Only poured 30 plates so far but it seems to work well enough (except for the inoculation itself lol). Only a few of them got some contam not related to the inoculation points. Hope I can get a work area as organized as yours one day!


Thank you :hug:

But this is not that professional, I am lucky to have the space for it and never have to pack anything away, but it's just a grow tent, shelves and totes sitting in my man cave :muppet:

If you could get armholes covers and prepare your SAB the evening before you do your work I would think you don't need to spray the SAB at all. Spraying a SAB has it's trade off to. Water particles can take days to settle. Soapy water is better then ISO in my opinion because it stays sticky a lot longer then ISO and particles would stick to the walls longer.

You can do both, wipe down the SAB with ISO, set it on the desk and then spray soapy water on the walls, but if you prep it the day before, use a rack and get armhole covers I'm sure going in dry is fine :muppet:

And yes, I have not yet learned to catch all embedded bacteria on a inoculated plate and I have to clean up plates, when I am in doubt I just run the culture through a cabin :awesomenod:

I am still struggling with grain prep :crankey: I do not know why, but my jars usually takes 3 weeks to colonize when I inoculate with agar, but time and patience is a big thing with this hobby, so I'll get it right some how, some day :lol:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29255222 -

I’m using a casing layer for the first time, is the casing supposed to colonize before I introduce FAE?


--------------------
~Wherever you go in life, there you’ll be~




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Re: Ask Quick Questions, Get Quick Answers [Re: noomer]
    #29255233 -

noomer said:
I’m using a casing layer for the first time, is the casing supposed to colonize before I introduce FAE?


What are you growing? With cubes it's FAE from when you spawn to tub.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29255239 -

Nats


--------------------
~Wherever you go in life, there you’ll be~




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Re: Ask Quick Questions, Get Quick Answers [Re: noomer]
    #29255240 -

noomer said:
Nats


Actual Nats or Ochraceocentrata?


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29255243 -

Actual nats is what I was told


--------------------
~Wherever you go in life, there you’ll be~




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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical]
    #29255246 -

Tropical said:
Soooooo I was all set on going spore print --> syringue --> pf cake

then some knowledgeable users told me it's a caveman method and just be a big man and go agar.

Sooooo I was all looking at agar BOD tek, thinking sweet it does not look that hard.

Then i've stumbled upon a thread stating pour agar with SAB = misery.

So as you can look from the previous posts i'm looking at some FFU unit, but it'll take quite a long time to get to me.

So long story short :

1. Is SAB + agar pouring = misery? Or it's doable?

2. Or just look at no pour tek?

3. If I should follow a no pour tek, which one is the best?

Cheers


I’ve just recently done my first agar pours in a SAB. Out of 16 unused plates I had 2 with contams. Only real issue I had was a little bit of condensation to clean up but honestly it's easier than you think and definitely doable. Bod has some helpful videos I recommend you watch.

Having said that idk if it's just my noob technique or maybe I got unlucky with a dirty print but all of my swab plates were pretty much unusable. Contams out the wazoo. So I’m going back to good ol' tried and true brf to get something to clone with. As for making syringes I just scrape a small section of spore print into some sterile water and suck it up with a fresh syringe. Nothing to it really.

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Re: Ask Quick Questions, Get Quick Answers [Re: noomer]
    #29255254 -

noomer said:
Actual nats is what I was told


Well shit, then I got nothing, Oh wait I have one thing, have you checked the nat thread?


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29255256 -

I haven’t. I’ll head there now thank you!


--------------------
~Wherever you go in life, there you’ll be~




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Re: Ask Quick Questions, Get Quick Answers [Re: noomer] * 1
    #29255267 -

Probably dumb question but that's how I roll.

Do you guys trust a PC with a jiggle weight and no pressure gauge? I messed up my 16 qt that had a gauge and I can't find an affordable replacement that has one


--------------------
Where to start?

Are your plates clean?


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Re: Ask Quick Questions, Get Quick Answers [Re: boxtrotalpha] * 5
    #29255268 -

The weight is more accurate than a gauge


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Ask Quick Questions, Get Quick Answers [Re: catnip40] * 3
    #29255275 -

:whathesaid:

My pc gauge stopped working over a year ago and o never bothered to replace it because my weight is correct.

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Re: Ask Quick Questions, Get Quick Answers [Re: boxtrotalpha] * 1
    #29255274 -

boxtrotalpha said:
Probably dumb question but that's how I roll.

Do you guys trust a PC with a jiggle weight and no pressure gauge? I messed up my 16 qt that had a gauge and I can't find an affordable replacement that has one


The weight is probably more accurate then the gauge out the box :awesomenod:


Thanks a LOT Catnip :crankey:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD] * 3
    #29255285 -

Ive never even used a gauge - only jiggle weight. All I've heard is they can be inaccurate anyway, so didn't see the point. Can't really get any easier than "weight jiggle good, weight no jiggle bad." 🤷‍♂️


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: boxtrotalpha] * 2
    #29255287 -

Well damn, I wouldn't have guessed that. Thanks friends


--------------------
Where to start?

Are your plates clean?


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29255289 -

so long as you know the regulator is the correct weight to reach 15psi

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29255293 -

Kloakk said:
If you could get armholes covers and prepare your SAB the evening before you do your work I would think you don't need to spray the SAB at all. Spraying a SAB has it's trade off to. Water particles can take days to settle. Soapy water is better then ISO in my opinion because it stays sticky a lot longer then ISO and particles would stick to the walls longer.

You can do both, wipe down the SAB with ISO, set it on the desk and then spray soapy water on the walls, but if you prep it the day before, use a rack and get armhole covers I'm sure going in dry is fine :muppet:



I started spraying and wiping the SAB with ISO as well, then I also got concerned that it wasn’t sticky enough. However the combination of both seems to be the safest route to go, lol.
Unfortunately I threw away the circular cutouts after making the armholes, they would have been perfect for covers. Great advice! A rack sounds great also, will need to get one along the way.
Thank you and also thank you nektar!

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Re: Ask Quick Questions, Get Quick Answers [Re: Fauchinger] * 3
    #29255310 -

magnetic vent hole covers make for good sab hole covers and i eventually just ended up running the SAB dry

once ur confident in ur sterile tek and ur comfortable working inside it you really dont need to spray it with soapy water


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Ask Quick Questions, Get Quick Answers [Re: catnip40] * 8
    #29255334 -

i got so much flak back when i posted my dry sab workshop thread.

of course i was being a psychopath about it but haah. good times.


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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