tryptkaloids said:

magicschoolbus said:

Mycelium or nah? Spore print to swab to pda, streaked 3 days ago

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Doesnt look great, but maybe

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Here's another angle, kinda looks like something is growing towards the center in the off color spots. But not the same as the stuff near the side of the jar. I used a suppperrr concentrated spore swab, you can see the spores under the questionable growth in the first Pic.
I started 3 other sets of agar, 1 with some old swabs of my own, 2 with some from a giveaway. This 4th set is the only one to show any kind of growth, and was made by taking fresh spores from a print and clumping them up on a swab. The other sets I could not see any spores streak onto the plate and its been about 3 weeks now with no visible growth. So the fact that something took off in less than 72 hours with this set makes me wonder.

Also the stuff on the walls of the jar is previous agar bits, not sure if the abnormal looking growth is aerial mycelium reaching for it? Idk

Dr. Smoke said:

callmehippie said:

Nope.  Absolutely no foil.  Fuck foil.  Lol

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Well, there's your problem.  Permeable filters allow moisture in to the jar while sterilizing, unless you're using a one-way filter like tyvek.  You need foil, my friend.  And if you use tyvek, put it on the right way, to allow internal moisture out and gasses in/out, and keep external moisture out, in which case you do not need foil.  You're sterilizing with steam.

humble student said:

PF Tek jar pinning AND not 100% colonized.

Birth now, or wait for 100% colonization?

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Not 100%?  Could be contam.  But still I'd GO! GO! GO!  Try to exclude uncolonized.

Meanwhile said:

Got a question that may be a no brainer to some but not for me. Basically I mistimed my grain prep, the plate I was intending on using is pretty much close to or at the side of the plate. It's not growing up it. Am I being ocd or should I be concerned about contam on the walls of the plate and just cut a circle around it and only use the center of the agar? Plate prep was done in a sab and left  for 2 weeks prior to use, all other work done in front of a tested ffu.

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Assuming you'd bought sterile plates, which you should always look for, it's not a problem to crawl up the sides.  But still it's time to shoot it to grain.

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Mub said:

Has anyone ever used the cabellas pro 5 dehydrator? Trying to make sure 165 and 24 hrs is the way to go. The manual says for mushrooms 4-10 hrs at 130.

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callmehippie said:

Lol.  Foil doesn't really do much. I've been running it without foil so many timed for a year. Lots of people have given up using foil. Any ways this is the first time I've had the issue. And why would it only just be the one jar and not all of them if no foil  was the problem right?

Like another member said though If i do use foil.  I don't individually wrap each jar with Just one big sheet across the top of all of them.

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Mub said:

Thanks. Yea by everywhere just in the container all over the shrooms and sub. It was wild, saw one veil about to break Sunday night. Than boom, Monday after work fuckin spore city man.

I could be interested in that fees six ant dehydration process. How much did that setup cost you about?

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nektar61 said:

You're not a radical, you're just wrong about heat destroying alkaloids.

25 years of experience from everyone here and 5 years of experience form me says you're wrong.

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nektar61 said:

The shrooms I dried like that that were gross mush in a closet later says your wrong.

Not using dehydrator never makes them tonally dry, which invites bacteria to do their thing, even if stored with no oxygen.

Rot entirely destroys potency, and just makes shrooms into a gross mess no one would not want to eat.

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nektar61 said:

You're brand new and giving misinformation. I'm putting you on ignore, and others should do the same.

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Dr. Smoke said:

  Never heard of spongey stipes with driers?  There is a reason for spongey, but it's a secret now, as well as what to do after.

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Mub said:

Has anyone ever used the cabellas pro 5 dehydrator? Trying to make sure 165 and 24 hrs is the way to go. The manual says for mushrooms 4-10 hrs at 130.

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24 hours at 165f is perfect. I have tried 170f and 175f as well.

Disregard Dr Smoke on this.  That herb drying rack is not the way to go.  He having an emotional overreaction, and trying to flex for internet points.

If heat killed the actives, then how are we all tripping balls after boiling tea at 212f?  I have put tea in the dehydrator for 2 days until it was instant crystals.  Stored in the freezer or made into gummies, and get high as giraffe pussy from that too. 

tryptkaloids said:

Why you gatekeeping?

Dr. Smoke said:

  Never heard of spongey stipes with driers?  There is a reason for spongey, but it's a secret now, as well as what to do after.

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Nah, he being the self proclaimed adult here.

tryptkaloids said:

Why you gatekeeping?

Dr. Smoke said:

  Never heard of spongey stipes with driers?  There is a reason for spongey, but it's a secret now, as well as what to do after.

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ThurmanMurman said:

I was getting plenty of water pooling on lids even with foil. The foil just becomes the condensation point instead of the PC lid. At least that's what makes sense in my mind anyway.

If one is getting excessive water inside the jars from the steam in the PC, shouldn't that be a filter leak? My SFDs have a waterproof side, which I put against the lid when I siliconed. Water on the lid or not, I'm not getting water in my jars during sterilization. I'd think either the water is soaking through or getting by a leak in the silicone, no?

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That's exactly how it was explained to me and makes sense in my mind LOL just another condensation point. That's why this time I actually didn't put the big sheet across the top of all of them I was like isn't this just another roof closer to the lids now for the condensation to build up and drip off of.

I'm definitely leaning towards the filter as well. It also had some runoff Sharpie that was still on the lid getting mixed into it maybe that loosened it up a little. Seen as it was only just that one.

And yes they do make hydrophobic filters you just have to pay attention and make sure those are the ones you're getting and that you're putting the right side down.like you are NOTY

Tropical said:

hello,

Hope everyone is doing well!

1. would those petri dishes work for agar agar? https://www.amazon.com/Pyrex-3160-101-Brand-3160-petri/dp/B00141WFNE

2. Anyone knows if they are ok for agar agar? (LME recipe)

3. Any reason to go for plastic petry instead of glass ones? (except price ofc)

4. With 10 petri dishes, anyone have an estimate on how many pucks you can do with 1 dish? (https://www.shroomery.org/forums/showflat.php/Number/28649340#28649340)

5. For the agar recipe, i've found this way to do it : https://www.shroomery.org/forums/showflat.php/Number/28354611#28354611
is it still relevant and OK?

6. For pucks, all i have to do is follow https://www.shroomery.org/forums/showflat.php/Number/21922023 then put some on the pucks and that's ok? Or is there another step?



cheers!

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