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Re: Ask Quick Questions, Get Quick Answers [Re: sprnk] * 1
    #29234198 -

Man, you know how much I cried at the end of charlotte’s web?


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29234207 -

Mr Piggy said:
Man, you know how much I cried at the end of charlotte’s web?



When I read it last I was maybe 10 years old. I missed a lot of what was going on there. I'm in tears right now just reading the synopsis from wikipedia. I didn't get it as a kid. I get it now.

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Re: Ask Quick Questions, Get Quick Answers [Re: sprnk]
    #29234543 -

What size cooler would you get for doing better insulated bucket teks? So far bucket tekking with straw has been working, but I often have to top up with more water to keep it within range.

Also, when albino strains like Yeti or APE have tried, their caps usually turn yellow. AJMF dried white. Is this the difference between albino and leucistic, or something else?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Edited by Huskies (05/31/25 12:25 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 1
    #29234581 -

I think mine is 5 gallon / 20l

Enough for 2 bricks and verm, without getting too tight.

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Re: Ask Quick Questions, Get Quick Answers [Re: Dkeppel]
    #29234693 -

Dkeppel said:
Quote:
My daddy has the IBS.
Had a fever as a lad and needed to have a piece of intestine removed.
He drinks Lactaid in place of any milk.
Might be a good place to start.




I do need to pay more attention to my diet.

It's so expensive being gluten-free, feels like everything cheap and filling has gluten :mad2:

Quote:
I got IBS pretty bad.  Making tea and not consuming the material helps a bit, but the older I get the worse the results.  They're harsh on the tum, that's for sure.




Tea made with potent mushrooms is my usual go to but I've been unusally unwell for months, which puts me off tripping and feeds the cancer is back brain worms, which is not an ideal headspace.

I'm hoping a good trip might knock me out of this self pitying rut.




Damn DK.

I've never been diagnosed but I'm sure I have some sort of stomach Affliction as I've dealt with it my entire life. Always nauseous always having my stomach interrupting my activities.

I feel the same when it comes to tripping. I'm always trying to find the right time for my stomach. And having to deal with lots of nausea even if I do think it's the right time.

Hopefully we can all get this figured out together and find some sort of way to help alleviate it a little. Some sort of way other than tea. i mean. something we haven't necessarily tried.

I wonder if taking some zofran or promethazine before the trip would help or if that would have adverse side effects?

As far as your mental state. Just don't hold it in.  keep coming here and talking things out with us.

Sometimes  things seem worse than they are. Or sometimes we get stuck in things being worse and make them even worse than they are. And it becomes a downward spiral.

Our mind is powerful and it can work for or against us. Sometimes being conscious and telling yourself I would rather my mind work for me then against me is the little bit of nudge needed to change some things around.

And then you got to put some real work into being optimistic instead of pessimistic. Sorry if I'm ranting I hope this makes sense. This is just kind of the way I look at things and it helps.

It's hard to find a positive trip when everything is leaning more towards a negative connotation..

I think sometimes that  can be the hardest part. Just getting your mind right.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29234789 -





So on the 26th (5 days ago) I pasteurized some substrate (hpoo/straw/verm) using my sous vide.

I left the substrate inside the ziplock bag I used during the pasteurization (150F). It was left at room temp for 5 days without being opened and didn't have any GE as the ziplock bags was closed. Temps was around 25c.

I'm curious as I don't believe I didn't pasteurize correctly as I done it before and hard to fuck up using a sous vide:shrug:

Was it the fact that there was no GE that made the substrate contaminate or at least what I belive to be contam? See pictures above.

Reason I didn't stress about using it right away is because of what Jakeoncid says in his write-up "The next day I take it out and set on shelf in Lab for the microbe count to Build. I prefer to use bulk that is at least 5 days old. The longer I wait the lower my chances of contams."

Any feedback is much appreciated.

It smelled very strong and has even white dots all over.

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Re: Ask Quick Questions, Get Quick Answers [Re: Puduwoke] * 1
    #29234803 -

How long did you pasteurize? You sure the center actually reached temp and was held there for a couple hours?

In any case, leaving it sealed is a big reason for failure. Pasteurization doesn't kill everything, and leaving it sealed gave the anaerobic organisms remaining a huge leg up. The beneficial ones tend to be aerobic.

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Re: Ask Quick Questions, Get Quick Answers [Re: Puduwoke] * 1
    #29234804 -

Strong smell of what? Does it smell sour? I ask because Mico Zapotec write about the importance of stirring the substrate and casing while pasteurizing to get oxygen in it. I use bags, but never submerge them, that way I can introduce oxygen and shake them several times during pasteurization.

"Also during pasteurization you should mix it several times to spread water and fill with oxygen.
When pasteurization finished also makes sense to keep it for a few days before doing casing. The substrate should not have any smell of sour!"


How are you reading the temperature of what you are pasteurizing if the bags are submerged?


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29234807 -

Phaethon said:
How long did you pasteurize? You sure the center actually reached temp and was held there for a couple hours?

In any case, leaving it sealed is a big reason for failure. Pasteurization doesn't kill everything, and leaving it sealed gave the anaerobic organisms remaining a huge leg up.



:snoopyes:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29234810 -

Phaethon said:
How long did you pasteurize? You sure the center actually reached temp and was held there for a couple hours?

In any case, leaving it sealed is a big reason for failure. Pasteurization doesn't kill everything, and leaving it sealed gave the anaerobic organisms remaining a huge leg up. The beneficial ones tend to be aerobic.



Followed Asuras write-up about it and put the substrate in from the start woth room temp water. Once the water hit 66C I set the timer on the sous vide for 4 hours. Obviously the core temp of the substrate isn't yet at that temp though.

I'm currently pasteurizing again and will take the core temp soon to see how long it takes the core to reach the correct temp.

Kloakk said:
Strong smell of what? Does it smell sour? I ask because Mico Zapotec write about the importance of stirring the substrate and casing while pasteurizing to get oxygen in it. I use bags, but never submerge them, that way I can introduce oxygen and shake them several times during pasteurization.

"Also during pasteurization you should mix it several times to spread water and fill with oxygen.
When pasteurization finished also makes sense to keep it for a few days before doing casing. The substrate should not have any smell of sour!"


How are you reading the temperature of what you are pasteurizing if the bags are submerged?



Not saying I don't believe or trust Mico. Not at all. But I never read or heard about anyone doing that before. Nor have I done it and I had success using a crookpot for many many grows so I don't think that necessary at all:shrug: might be beneficial though sure. But again. Never heard anyone or any of the teks on here every mentioned that.

Yeah smelled sour and so much like horse poo.

Edited by Puduwoke (05/31/25 03:18 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Puduwoke] * 1
    #29234848 -

Puduwoke said:
Not saying I don't believe or trust Mico. Not at all. But I never read or heard about anyone doing that before. Nor have I done it and I had success using a crookpot for many many grows so I don't think that necessary at all:shrug: might be beneficial though sure. But again. Never heard anyone or any of the teks on here every mentioned that.

Yeah smelled sour and so much like horse poo.



I don't think it is absolutely necessary, especially if you use the sub/casing right after it's cooled, might even be fine left alone for a week, most likely your sub wasn't pasteurized properly and that is why it's fucked.

I was just thinking about what may have caused it and think of what could increase the chance of success, for anybody that read this. I might be to involved in this hobby to a point where it's an unhealthy obsession, but I try to do everything I can to get the best conditions in all phases of cultivating, I mean, I certainly don't need more shrooms :lol:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29234883 -

If I had 1 of 7 jars massively contam without having been opened since the PC cycle, its lid failure, right?


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29234906 -

Kloakk said:
Puduwoke said:
Not saying I don't believe or trust Mico. Not at all. But I never read or heard about anyone doing that before. Nor have I done it and I had success using a crookpot for many many grows so I don't think that necessary at all:shrug: might be beneficial though sure. But again. Never heard anyone or any of the teks on here every mentioned that.

Yeah smelled sour and so much like horse poo.



I don't think it is absolutely necessary, especially if you use the sub/casing right after it's cooled, might even be fine left alone for a week, most likely your sub wasn't pasteurized properly and that is why it's fucked.

I was just thinking about what may have caused it and think of what could increase the chance of success, for anybody that read this. I might be to involved in this hobby to a point where it's an unhealthy obsession, but I try to do everything I can to get the best conditions in all phases of cultivating, I mean, I certainly don't need more shrooms :lol:




I just took the temperature of the substrate that's currently pasteurizing and after 2 hours it hit 62c in the center.

That leave those bags in question with 2 hours above 140f. I for sure pasteurized them properly.

This is why I would like a second opinion about it:shrug: Since I think the problem was that they didn't have any GE at all and temps where i stored it were pretty high.

I'm open to being wrong though.

To be sure I'm adding another 2 hours to the current batch.

I guess we Will find out soon enough if the trays I spawned today get contaminated or not though since I used 4 hours with those as well:prayer:

Dude btw, your brew area looks awesome:rockon:

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Re: Ask Quick Questions, Get Quick Answers [Re: Puduwoke]
    #29234923 -

Puduwoke said:
I just took the temperature of the substrate that's currently pasteurizing and after 2 hours it hit 62c in the center.

That leave those bags in question with 2 hours above 140f. I for sure pasteurized them properly.

This is why I would like a second opinion about it:shrug: Since I think the problem was that they didn't have any GE at all and temps where i stored it were pretty high.

I'm open to being wrong though.

To be sure I'm adding another 2 hours to the current batch.

I guess we Will find out soon enough if the trays I spawned today get contaminated or not though since I used 4 hours with those as well:prayer:

Dude btw, your brew area looks awesome:rockon:



Wow that's fast, I think my digiboil uses about 4-6 hours to get to temp, then I do 2 hours. Could be a lot I'm missing, I've only used cow manure compost, coir, verm and cactus soil.

Tomorrow that'll change, I'm doing 8 different substrate for 8 trays of TTBVI, guess I'll find out if I've just been lucky or if the way I've been doing it actually works :uhoh:

But if your substrate was properly pasteurized, then it would be interesting to know what went south. 60°C should kill all molds and yeast, so I'm wondering what is growing in those bags.

Thanks for the kind words on my brew corner, appreciate it :hatsoff:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29234970 -

Well pasteurization doesn't kill everything so I guess the environment I had without GE and the heat where I kept them grew something. When it comes to identifying contams I'm terrible. Trichoderma and I know each other all to well though:horrified:

Hopefully someone with more experience can shime in and tell us more in detail :shrug:

I always just want to find out the cause of my failures when they occur. Drives me insane when I fail and can't figure out what I did wrong.

I did raise the temps and the time this current batch though.

Will get back to you in a few days when I find out about the trays I spawned earlier today. If they work out fine. Then we know for sure GE was the problem since they were made exactly the same way as the once in my photos with contam in them:shrug:

Edited by Puduwoke (05/31/25 05:59 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Puduwoke]
    #29235245 -

I have a pf jar that is 90 percent colonized, it i looked at the back and there are tiny mushrooms and pins everywhere.

Should I open it up?


--------------------
The closer you get to the meaning the sooner you know that you're dreaming

How the Bushman gets stuff done

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Re: Ask Quick Questions, Get Quick Answers [Re: hidingbush]
    #29235280 -

Post some pictures. Your 90% is arbitrary. Do you have more jars?

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29235293 -

I do not have any more jars. This was from a syringe th

at was in my refrigerator for a year


--------------------
The closer you get to the meaning the sooner you know that you're dreaming

How the Bushman gets stuff done

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Re: Ask Quick Questions, Get Quick Answers [Re: hidingbush]
    #29235299 -

For lions mane bags, soy pellets or wheat bran to go with my oak pellets?

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Re: Ask Quick Questions, Get Quick Answers [Re: Arkyhog] * 2
    #29235316 -

Arkyhog said:
For lions mane bags, soy pellets or wheat bran to go with my oak pellets?



I use wheat bran because it’s what I can source locally, but I liked soy hulls better when I could get them. Neither are worth it if you have to pay shipping, though.

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Shop: Mushroom-Hut Liquid Cultures   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com APE Liquid Culture For Sale   North Spore Boomr Bag   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy


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