Shroom_Ed65 said:

do you suggest I pour a little water down the sides to moisten it back up?

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No, I wouldn't do that. How big is your tub? I've had 27qt tubs that have given me 2 full flushes like this (pictured is the 1st flush)

without the need to mist/soak, or anything. I just picked the first flush, popped the top back on and waited for my next flush. I think you'd be surprised (as was I, until I experienced it in person) how many flushes these bricks of coir can push out with just the original water you added to them to hydrate them

Shroom_Ed65 said:

yes I do check on that a lot, the mycelium does have tiny droplets on it, I'm just concerned about the pins turning black thinking that they are aborting, is it normal to have that many abort on the first flush when they are so small, less than an inch tall?

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Shroom_Ed65 said:

it's 28 qt. I'm pretty sure, I try and keep the sides wet and fan it a few times a day to give it fresh air.

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Huskies said:

SkanskKorv said:

Jrey16 said:

SkanskKorv said:

When do I chuck my jars and start over? I still have no mycelium growth whatsoever after 17 days, I won't chuck them just now but I want to know how long is too long because I'd need the jars to try again. I was thinking 4 weeks before I throw em out judging from what I've read, or should I perhaps wait longer?

They're pan cyan TTBVI and I used Shea25's pans without a pc guide.

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I have TTBVI on grains right now fully colonized ready to S2B. It took around 2 weeks to fully colonized. 17 days with no growth is too long. I would start over. I saw growth within the first day or 2. There is for sure something wrong with your culture or grains. Pretty sure it is the culture. Did you use LC or agar? I used agar. I've tried making LC before but that's harder in my experience. With agar you can see if the culture is clean or not.

Oh and I just noticed you said you didn't PC the grains? I tried that many times (broke boi tek) when I first started and it was always a fail. Once I got a PC (I bought the cheapest one) I finally started getting successful grows. All of the pictures in my signature are from my first ever grows.

I haven't grown pan cyan yet, I am still new to it. But it is more complicated than growing cubes imo. You need clean culture and make sure to sterilize your grains properly with the right moisture content in your grains. That is the key to success!

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No agar or liquid culture, it's an ms syringe so I expected to to take a week or so to show any signs but even for spores this seems like an awful lot of time.

And I didn't use grain spawn, as I mentioned previously I made a modified pf tek with 1:1:1:1 manure, verm, brf and water bit I suspect it's a bit too wet.

I made an LC from the TTBVI spores a couple of days ago though so I'll be trying that but it feels like a big risk, I just don't feel experienced enough for agar and feel a tad confused over it. I guess it's time to learn though... Is there any simple guide for it?

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What pressure cooker did you get? My sig has a pretty simple and cheap way of doing it in an instant pot, but that is for pouring. 1000ml of gnocchi water gets me around 80 sauce container plates and I do it in a SAB with absolutely minimal contaminations on the agar itself.

A even safer method would oldschool pastyplates, which you could also do in an instant pot or small pressure cooker.

spores to LC is not going to give you clean LC, so scrap that idea. LC is hard to make perfect, even more so from a beginner.

You are also using a 15 year old tek, things change, and a lot of old stuff has become outdated as the shared knowledge increases. Doesn't mean it can't work, but it means it's less reliably going to do so.

For Pan Cyans, you should try this. There is also a huge thread for them specifically here

Your best bet would be use spores to get clean growth on agar, make 1 or 2 transfer from good mycelium until you have good even growth, then use that to inoculate all in one jars with wedges or even by making a LI with PCed water,

You could maybe even re-sterilize the cakes you have now since there is no growth in them. Maybe someone else has a better answer in regards to if that will still work.

edit: you still might see growth from these cakes. If nothing is showing up, at least there isn't mold or bacteria, so in theory your syringe could've been sufficiently clean for pf cakes.

Leave them be, be patient. But I do recommend the links I gave you.

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Huskies said:

@OnlineSkanskKorv: that PC will be more than fine, but stick to smaller sized jars, half pints and pints at most. best of luck! do you still have spore solution left? You only need a tiny bit on agar to propagate infinite mycelium.

@Shroom_Ed65: were the grains colonized with spore syringes? Vendors push that, but it is not considered a good way to go in terms of sterility. Spore to grains are less likely to be very clean, and even vendor LCs need to be tested.

Give it time, patience tends to be the first casualty with your first grows. Hopefully you will get a nice flush.

It could be the case that it is slightly or even heavily bacterial, and that is why there aren't many pins or that they are aborting. Was there a fermented fruit smell to the spawn?

If so, bacterial spawn is your likely answer. Agar is the secret to reliable success.

Cheers and best of luck to both of you

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SkanskKorv said:

Jrey16 said:

SkanskKorv said:

When do I chuck my jars and start over? I still have no mycelium growth whatsoever after 17 days, I won't chuck them just now but I want to know how long is too long because I'd need the jars to try again. I was thinking 4 weeks before I throw em out judging from what I've read, or should I perhaps wait longer?

They're pan cyan TTBVI and I used Shea25's pans without a pc guide.

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I have TTBVI on grains right now fully colonized ready to S2B. It took around 2 weeks to fully colonized. 17 days with no growth is too long. I would start over. I saw growth within the first day or 2. There is for sure something wrong with your culture or grains. Pretty sure it is the culture. Did you use LC or agar? I used agar. I've tried making LC before but that's harder in my experience. With agar you can see if the culture is clean or not.

Oh and I just noticed you said you didn't PC the grains? I tried that many times (broke boi tek) when I first started and it was always a fail. Once I got a PC (I bought the cheapest one) I finally started getting successful grows. All of the pictures in my signature are from my first ever grows.

I haven't grown pan cyan yet, I am still new to it. But it is more complicated than growing cubes imo. You need clean culture and make sure to sterilize your grains properly with the right moisture content in your grains. That is the key to success!

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No agar or liquid culture, it's an ms syringe so I expected to to take a week or so to show any signs but even for spores this seems like an awful lot of time.

And I didn't use grain spawn, as I mentioned previously I made a modified pf tek with 1:1:1:1 manure, verm, brf and water bit I suspect it's a bit too wet.

I made an LC from the TTBVI spores a couple of days ago though so I'll be trying that but it feels like a big risk, I just don't feel experienced enough for agar and feel a tad confused over it. I guess it's time to learn though... Is there any simple guide for it?

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Mr Piggy said:

SkanskKorv said:

Jrey16 said:

SkanskKorv said:

When do I chuck my jars and start over? I still have no mycelium growth whatsoever after 17 days, I won't chuck them just now but I want to know how long is too long because I'd need the jars to try again. I was thinking 4 weeks before I throw em out judging from what I've read, or should I perhaps wait longer?

They're pan cyan TTBVI and I used Shea25's pans without a pc guide.

---

I have TTBVI on grains right now fully colonized ready to S2B. It took around 2 weeks to fully colonized. 17 days with no growth is too long. I would start over. I saw growth within the first day or 2. There is for sure something wrong with your culture or grains. Pretty sure it is the culture. Did you use LC or agar? I used agar. I've tried making LC before but that's harder in my experience. With agar you can see if the culture is clean or not.

Oh and I just noticed you said you didn't PC the grains? I tried that many times (broke boi tek) when I first started and it was always a fail. Once I got a PC (I bought the cheapest one) I finally started getting successful grows. All of the pictures in my signature are from my first ever grows.

I haven't grown pan cyan yet, I am still new to it. But it is more complicated than growing cubes imo. You need clean culture and make sure to sterilize your grains properly with the right moisture content in your grains. That is the key to success!

---

No agar or liquid culture, it's an ms syringe so I expected to to take a week or so to show any signs but even for spores this seems like an awful lot of time.

And I didn't use grain spawn, as I mentioned previously I made a modified pf tek with 1:1:1:1 manure, verm, brf and water bit I suspect it's a bit too wet.

I made an LC from the TTBVI spores a couple of days ago though so I'll be trying that but it feels like a big risk, I just don't feel experienced enough for agar and feel a tad confused over it. I guess it's time to learn though... Is there any simple guide for it?

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Buddy, none of that is clean tek.  You can't just throw manure in cakes, you have to pasteurize manure which you can't do with cakes.  If you mix and match or modify TEKS you are no longer doing that tek, you're just gambling.

Also you can't do spores to LC, I promise that is contaminated.

You need to start simple with learning to work agar, and then transferring clean cultures from agar to grain.  No more of this shortcut stuff, and don't worry about LC until you're growing in large volume and have the grow skill/wisdom to do the very clean work you need for LC. 

LC is miles up the skill chain from agar, agar is THE basic skill of mycology. 

Start with agar.

Agar.

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cronicr said:

Send'em

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Thanks, cronicr.