DV8DUG said:

Okay... I don't really have the free time to read through the 16k of posts in Woodlovers thread. Can someone inform me of the best spawn medium for Ps. Allenii? I have never grown a wood lover before and I have full plates all of a sudden.

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LewDoja said:

Have you had any issues?

I try to keep a dust cover over master jars, If I can reduce the overall amount of particles the better.... or just keeping them inside a tote or box during colonization is better than on an open shelf.

Even if it's just all in my head, it helps my peace of mind

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CranberrySteve said:

Hey shroomers, just wanted a sanity check on my thought process.

I’ve seen a lot of old posts about pre-adapting mycelium to grain or substrate at the agar or LC stage. The theory is that by exposing it early, the mycelium learns to produce the right enzymes, grows stronger, and becomes more resistant—skipping the usual adjustment period when transferring to grains or bulk.

Could I not take this further by starting with multispore on agar made from the same grain and substrate I’ll use in the grow.
Incubate the agar in the exact same conditions as my colonization/fruiting environment.
Why spores instead of a clone or LC?
Spores = millions of genetic variations.
Some of those genetics will naturally thrive in my specific setup (nutrients, temps, etc.).
By isolating the strongest mycelium through transfers, I’m essentially breeding the perfect genetics for my grow conditions.

Does this make sense, or am I overcomplicating it?

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That pretty much is what I have been doing lately, at least for spore to agar, my plates do live in my grow room. I don't always use Rye water mostly because I've been trying different things.

But... how do you make agar from substrate?

psilygoose said:

That pretty much is what I have been doing lately, at least for spore to agar, my plates do live in my grow room. I don't always use Rye water mostly because I've been trying different things.

But... how do you make agar from substrate?

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Damnlamb said:

Wondering if anyone can confirm if this is contamination or not, first time I've experienced these symptoms which are: really fluffy mycelium or mold that has attached to tub walls and a fairly strong vinegar/ferment smell. The why is easy for me to guess, they were accidentally deprived of fae while I was traveling but unsure if should be binned or not

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ThurmanMurman said:

Im doing g2g with an ffu and pretty much letting the jars sit in the flow for 5min, rotate and sit another 5, and then wipe down with iso. My main focus is lids of receiving jars and then a good wipe down on donor. I'm still not really a fan of having the donor jar hovering over the open receiving jar, but this is pretty much all I can do, right? I try to put some distance between the jars while I pour, but idk...it still feels sketchy to me. I haven't done much g2g, so I may just be being paranoid?

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With sterile laminar flow it doesn't matter much if you're hovering over the opening. The stream of air won't really let any nasties fall straight down.

Just don't put anything non-sterile between the filter face and an open sterile thing.

I just wipe the lids (if I remember ) and go, no waiting.