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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29169163 -

callmehippie said:
But how do u transfer the pin from wherever it sits at to the sab without worrying about contamination on the way? I'm asking just so I don't have to carry the whole shoe box over and put it in there or is that the only way?

The albino varieties don't usually drop spores do they?



Why can't you carry the shoebox? I'm an old lady and carry shoeboxes all the time. Sometimes 16 quart boxes too. It's not going to hurt it to move it.

Just move the shoebox near the SAB (or set up the SAB near the shoebox), flame sterilize a scalpel, and cut it off above the base. Cool the scalpel agar near the edge of the plate you're transferring to or you'll be cooking the mushroom when you slice it.

Is that a true albino variety? I guess if it's TAT. Not all white shrooms are true albino, (Albino A+ for example)

I don't grow albinos, they look freaky to me. I like my mushrooms brown. Did grow TAT once, and I got a swab, not a print.

Albinos make spores, they can be clear. They don't always drop spores.You can swab some spores off the gills once they open if all you're trying to do is have a way to give them away, or save for later.  PEs, while not albino, are the same way.

Only albinos I grew were classic TATs 4 years ago and I got them as a spore swab. If all you're trying to do is have the ability to grow more after they're done, just get some sterile medical swabs on Amazon, wait until the shroom is mature, then swab all under the gills, dry in your SAB and bag it.

I thought you were trying to learn to clone.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (03/26/25 02:50 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61]
    #29169176 -

Lol. Of course I can move the box. I'd just rather Not,if I don't have to. I mean why take the whole box if I can just take the pin. 


Just trying to learn things and if there's easier ways to go about it. 

I used all that I had of this culture on these cakes. So I do need more and I just saw the opportunity to try somthing new.  So technically I'm not cloning for a specific characteristic or anything at least not yet. 

Also.  My main thing is I'm wanting to go about this in ways I havnt done before.


The only way to learn new things is to try them. My mind set it to learn everthing. Practice/perfect everthing. And try everthing. Just for the sake the experience/knowledge. 

But yes I am trying to learn to clone.  Only im trying a way I havnt already done is all. 


And thats good info about the albino spores.  I was  confused. About them not having spores as opposed to not dropping spores. So you definitely cleared that up for me.

Im sure I'll end up just taking the box over to the sab though :grin:


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 3
    #29169250 -

callmehippie said:
Lol. Of course I can move the box. I'd just rather Not,if I don't have to. I mean why take the whole box if I can just take the pin. 


Just trying to learn things and if there's easier ways to go about it. 

I used all that I had of this culture on these cakes. So I do need more and I just saw the opportunity to try somthing new.  So technically I'm not cloning for a specific characteristic or anything at least not yet. 

Also.  My main thing is I'm wanting to go about this in ways I havnt done before.


The only way to learn new things is to try them. My mind set it to learn everthing. Practice/perfect everthing. And try everthing. Just for the sake the experience/knowledge. 

But yes I am trying to learn to clone.  Only im trying a way I havnt already done is all. 


And thats good info about the albino spores.  I was  confused. About them not having spores as opposed to not dropping spores. So you definitely cleared that up for me.

Im sure I'll end up just taking the box over to the sab though :grin:



I don’t understand what your ziplock is doing for you. We spawn in open air. Your shoebox, while lidded, is still allowing air to circulate into it. Whatever is in the air between your shoebox and your SAB is already on your mushrooms. You’re not protecting them from anything that’s not already on them. :shrug:

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29169257 -

callmehippie said:
And thats good info about the albino spores.  I was  confused. About them not having spores as opposed to not dropping spores. So you definitely cleared that up for me.

Im sure I'll end up just taking the box over to the sab though :grin:



Another thing, in addition to "they don't drop spores, you need to swab them to get spores" is that they also don't produce as much spores as a lot of non-albino varieties. Sometimes very little.

As far as I know, the only cubes that have to be spread by cloning because it produces zero spores are those weird super strong mutant varieties that don't even look like mushrooms. They look like a brain or coral. They have no gills thus no spores.

They also grow very slowly. Maybe why they're stronger, since PE grows slowly and is strong)

Enigma is the name of one. I think they've only been around about 5 years.

Here's a pic of some Enigma (by InfraredRick)
:

Whenever new growers (or old growers) parrot something about senescence being something to worry about here, I bring up Enigma.

If senescence was a big issue in this hobby, that variety would be getting weaker and less robust every time it's cloned.

Here's a more typical looking one (from anonymous poster)


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (03/26/25 07:04 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29169294 -

GenesisCorrupted said:
If you’re looking to chill out. How much DMT would you sprinkle on a bowl of marijuana?

I know a lot of people are confident and they can just eyeball it.
Please give me a comparative amount to look for.
Like the top of a tack.



For a happy chill rather than an adventure, allowing for inevitable wastage using that ROA I'd go for 25mg.  (I'm working on the basis that you're doing the sandwich method - ash at the bottom, DMT sprinkle, weed on top, heated rather than lit directly with the flame).  What that looks like is tough; I've had batches that were dense and others fluffy.  A couple of grains of long grain rice mightbe about right - the good thing is you're unlikely to take it in one so if the first toke hits hard, just go with it.


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29169309 -

First off welcome!

So you're saying you scraped spores to agar, which had too much nutrients...how much is too much?

...took 2 transfers (to new plates)

...then you put (what) into fruiting?

But aren't sure that you actually put mushroom myc, or if your substrate wasn't clean??

Based off just the pics, I'd say you have mushroom mycelium growing on coir.

Could it be something else?

Yes there could be something else alongside it*.... doesn't necessarily mean that there is.


Edit:
After looking at your other posts, how did the germination plate turn out from taking gill fragments off a dried specimen?

Like how soon till noticeable growth, was there any contamination?

The grain spawn looked a Lil sus in the last 2 pics and 40% within 3 days seems like almost too fast for a2g, but not impossible.

What was the s2b methods/ratios?




Hi, thanks a lot, sorry for the delay, i really don't know the exact content of the agar, i used water in which i boiled a diverse mix of grains that i ate,that container has now been in fruiting conditions for 10 days, no foul odors or colors, but no pins yet either

The germination plates did eventually(after transfers, t2 and onward then were cleaner, no visible contams) contam green

I did use like 6 agar wedges per jar, which may have been a mistake,i spawned a smaller jar onto that small container in a 1/1,5 grain(oats) to CVG ratio, then a few days later spawned the 3 cleaner jars onto a monotub,which has a mix of denser and fluffier myc, a tiny spot of metabolites but no foul odors or colors





This is the 10th day into fruiting conditions, i hope i see something soon

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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16] * 1
    #29169313 -

Jrey16 said:
Would any of these be okay to put to grains? Mr. Peanut first pic, and second picture Golden Teacher








Mr. Peanut... 2nd row plate on right is clean. The rest are hard to tell because of background but it looks like you may have a second mycelieum growing in there.

GT's... 2nd row, both plates and 3rd row the plate on the right.

Have a look at THIS. It'll show you how I draw my conclusions.

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 1
    #29169500 -

Can someone point me toward a recent tek for making spore syringes?  Preferably something stipe approved lol


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 5
    #29169505 -

Nothing wrong with ye old shot glass.

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD] * 1
    #29169564 -

:whathesaid:

Don't over think it...

Boil water, aspirate,  expel.... rinse and repeat. 

Can also pc with water, with a needle on it might lose some water...
with luer-lock cap probably not.


Sterilize shot glass and or jar of water.

Scrape into solution.  Aspirate into syringes

Edited by LewDoja (03/26/25 02:16 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29169568 -

if you pc with the needle in water you won’t loose water.

i put them in a small mason jar of water and it works well

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Re: Ask Quick Questions, Get Quick Answers [Re: san pedro guy]
    #29169569 -

I always leave them at least a week. Just to be sure.


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 6
    #29169572 -

Is this better?



:tongue2:

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #29169577 -

I'm about to transfer agar chunks into mason jars of grain. I've seen this in guides, but either I've failed to notice or maybe it isn't mentioned, but what size mason jar is best? 16  oz? 32 oz? Any help will be much appreciated.

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Re: Ask Quick Questions, Get Quick Answers [Re: towerofbabel] * 2
    #29169583 -

32 oz are preferred.

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD] * 1
    #29169660 -

DERRAYLD said:
Is this better?



:tongue2:



Much thanks :lol:

I guess what I was looking for was I saw him talk about using a drop of dish soap to keep the spores from clumping, and we use like natural dish soap.  So I guess my question was if all dish soap is created equal in that regard?

Also, I will probably squirt water onto the prints and suck it back up, like I see folks do with serial dilution.  Make two at a time and pasteurize one.  Keep the other one in case I fuck that up.

Maybe I will bug stipe in the pasteurized spores thread.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 4
    #29169669 -

Polysorbate(tween20) and jetdry(rinse aid for automatic dishwashers) are surfactants used for that purpose, I believe. That may be the "dish soap" reference you're thinking of.


--------------------
This hobby has taught me many things.
Most of those things are patience.


The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

Edited by Scemilope (03/26/25 05:39 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Scemilope] * 1
    #29169700 -

Yeah that's what I wanted was the jet dry thank you!


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 2
    #29169705 -

1 gram agar to 1 liter h2o. Save the tween and soap for other tasks.
:pointmade:

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Re: Ask Quick Questions, Get Quick Answers [Re: the_chosen_one] * 2
    #29169719 -



Make one of these and you can print in a PF jar nice and sealed. Won't need an SAB or hood. Things are only exposed to air when you put the cap in and when you dump it all out. I have an excellent success rate making syringes this way.. even in my kitchen lol.

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