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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29139851 -

I have done a couple middling hits, but I was using a concentrate pen and it wasn’t up to the task and clogged.

I have a reliable dab rig that I am able to use.
I am still thinking of just making a cartridge, but I haven’t gotten the ingredients.


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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted] * 1
    #29139907 -

Around how many plates can you pour with a 500 mL bottle? assuming you're not pouring super shallow or super deep just average depth.


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 5
    #29139909 -

I pour 20 90mm plates or 40 60mm plates from 400ml of agar media (so about 20ml agar/90mm plate or 10ml agar/60mm plate). I don't think they are particularly thick or thin - probably about halfway up the dish, maybe 7 or 8 mm.


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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon] * 1
    #29139920 -

I like your style. I do about 20 x 60mm from a 250mL bottle.

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Re: Ask Quick Questions, Get Quick Answers [Re: Dkeppel]
    #29140043 -

Poured my agar plates yesterday. I let them sit over night and looks like I sm going to have to re heat some because some didn’t come out very level. I wrapped them with this micropore tape:

https://www.amazon.com/gp/aw/d/B0B2RFX4T7?psc=1&ref=ppx_pop_mob_b_asin_title


These are the dishes I used which say autoclaveable:
https://www.amazon.com/gp/aw/d/B0C9551NSZ?psc=1&ref=ppx_pop_mob_b_asin_title

My question is would it be a huge mess if I just put some back in the instapot to sterilize? The lids on the dishes are a little loose and aren’t the kind that just sit on as opposed to screwing on. I’m assuming I would have to take the tape off before I do that.

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub]
    #29140060 -

Agar recipe:
1l water
50g oatmeal
20-25g agar (cake, not powder-- I read one post recommending to go high)

How much corn syrup if no other amendments?? I know this is in the forums somewhere but I can't find it :confused:

Edited by maplepine (02/27/25 09:04 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub] * 2
    #29140197 -

maplepine said:
Agar recipe:
1l water
50g oatmeal
20-25g agar (cake, not powder-- I read one post recommending to go high)

How much corn syrup if no other amendments?? I know this is in the forums somewhere but I can't find it :confused:


Corn syrup can be used as a nutrient, if you're interested in recipes see Fastfred's media cookbook.

Mub said:
Poured my agar plates yesterday. I let them sit over night and looks like I sm going to have to re heat some because some didn’t come out very level. I wrapped them with this micropore tape:

https://www.amazon.com/gp/aw/d/B0B2RFX4T7?psc=1&ref=ppx_pop_mob_b_asin_title


These are the dishes I used which say autoclaveable:
https://www.amazon.com/gp/aw/d/B0C9551NSZ?psc=1&ref=ppx_pop_mob_b_asin_title

My question is would it be a huge mess if I just put some back in the instapot to sterilize? The lids on the dishes are a little loose and aren’t the kind that just sit on as opposed to screwing on. I’m assuming I would have to take the tape off before I do that.


The grafting film will melt so definitely take that off.

I believe a few people will stack and wrap their autoclavable petris with tin foil and keep them upright in the PC.

I run 60mm plates.


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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29140231 -

LewDoja said:
If you make it as an all in one (AIO)... to keep from contaminating prior to full colonization

Quote:
Now the LC or agar might be fun to look into! That I need to figure out how to apply.


Dont worry about LC... agar though most definitely 👌.
Stipes "why agar".

Quote:
I took the chance of tin foil and cracking the lids. The lids were modified and I saw some folks say the micron filter can do vacuum things from sterilization. They all seem to be bouncing back but only time will tell.



Not sure what al this means, with the last sentence "bouncing back" right after "sterilization"????




I had already done the sterilization process for 16 jars. 4 seperate cultures used. Had a micron filter and injection point on lids

Out of all of them, two fully colonized while the others were iradict pace and no signs of contam and only at like 10% each. The mycelium that was there looked healthy.

I had read some folks say things about the filter sometimes vacuuming during the sterilization so I decided to wipe down tinfoil and place it over the lids and give them the quarter turn for GE

The APEs are now after 3 days about 30%, B+ is 50%. It’s only the TW that’s dragging so those just might be lost causes


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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29140296 -

Orale vato!

How big of holes in the lid, what was the material used for filters?

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub] * 1
    #29140308 -

Just to clarify that is grafting tape,

This is micropore tape.



Not that it would have been better,  just a terminology clarification.

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29140319 -

Ah. Thanks for clarification.

But it was a 3/18 bit. The filter was a micron filter .22


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Edited by Splittinghairs (02/27/25 12:47 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29140339 -

GenesisCorrupted said:
I have done a couple middling hits, but I was using a concentrate pen and it wasn’t up to the task and clogged.

I have a reliable dab rig that I am able to use.
I am still thinking of just making a cartridge, but I haven’t gotten the ingredients.



It probably just needed to be warmed up i n front of a heater for a bit... did it look waxy ?

FYI it will cause your dab rig or nektar collector to stink. So might be good to get a dedicated one or look into the discrete emesh if you're unfamiliar with Minty Love has great information not just on the emesh.

If you're interested in anything else about the process from start to finish (extraction to blastoff) Lmk

Edited by LewDoja (02/27/25 02:09 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29140354 -

Hmmm ok. Is it worth even using the grafting tape than?

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub] * 2
    #29140360 -

Mub said:
Hmmm ok. Is it worth even using the grafting tape than?


I use parafilm, which I believe is very similar to grafting tape. It leaves no sticky residue like micropore tape can sometimes.


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:sporedrop:L.A.G.M. 2025:sporedrop:
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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29140372 -

Brf? Or grain? & Size of jars?

As smaller ones are better for brf and larger ones tend to stall

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29140388 -



Does this look good? It was cased 11 days ago and is in coco coir. It doesn't smell shroomy - doesn't have much of a smell at all. it Hydrogen peroxide doesn't hurt it. Seems very fuzzy.

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub]
    #29140389 -

Mub said:
Hmmm ok. Is it worth even using the grafting tape than?


I use the grating tape on eppendorf vials since it's stronger than pastic wrap that I use for plates. Some use parafilm  on plates just more expensive and prone to drying out and cracking.

The sticky microphone is used on lids (ge) or tubs to cover fae holes.
I wouldn't use it for plates as the adhesive might be detrimental to the cause.

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Re: Ask Quick Questions, Get Quick Answers [Re: tom of alchemy]
    #29140392 -

Looks good for the most part. Did it smell shroomy when spawned?

What tek did you follow for that tub?

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29140397 -

Huskies said:
It was pretty quick tbh, but I don't remember. Would you guess its based on how long it was?

How many days is it for spore to show growth  if they are fresh vs something like accidentally cloning gills? I assumed the quick growth was because they were fresh and stronk.


Hard to say but I'd bet on clone to be quicker than spore but also have different morphology than spore. Did you take pics of the plates before they got fully colonized?

Quote:
Now I'm wondering if most of my swabs have ended up just being that hehe. Shit.

I'm lucky I have spore prints though. I guess I'll have to grow those out to compare. If the spore print did legitimately yield the same results, would that be an oddity?


Not necessarily as we have many varieties now that were conceived from mutations at one point.

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29140410 -

I bought a fan filter unit a couple years ago, the first time I tried it, got contamination fairly quickly on agar plates.

I decided to give it a go again, set up a clean plate in front of the FFU for 10 min, and wrapped it in parafilm.

My question is how long do I need to wait to confirm that there has been no contam on the plate? It has been 8 days in the 70s with nothing growing on it. Is it safe to assume at this point I am good to start doing transfers in front of the FFU?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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