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Invisiblefastfred
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FastFred's Media Cookbook (v0.97) Now 60 Recipes, Indexed!
    #2996232 - 08/12/04 05:27 PM (12 years, 3 months ago)
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FastFred's Media Cookbook (v0.97)(8-30-2004)
--------------------------------------------------

by FastFred and ChesterKarma


...Index...
Section A. Classic Favorite Recipes
------------------------------------
Potato Dextrose Agar (PDA)(FDA M127)
Potato Dextrose Yeast (PDY)
Malt Extract Agar (3%)(MEA)(General Microbiology)(FDA M93)
Malt Extract Agar with Yeast (2%)(MEAY)
Sabouraud's Dextrose Broth and Agar (SabDex)(FDA M133)

Section B. Special Blends
--------------------------
"Karo Water" (Corn Syrup Broth)
"Dextrose Tek" Liquid Media (Corn Sugar Broth)
Honey Water (Mycotopia Honey Tek)
Malt-Yeast-Peptone Agar (McKenna's MYP)
Malt Yeast Peptone Agar (Stamets MYP)
Grey Cardboard (instead of agar)
Oatmeal Flake Agar
Moonflower's Rice Malt-Alfalfa-Brewer's Yeast Agar
MycoPsycho's Liquid Mycelial Culture Broth [Malt base]
Ragadinks Liquid Medium
Amaranth Soy Agar
EntheoGenesis No.442

Section C. Food Based Recipies and Variations
------------------------------------------------
Corn Meal Agar (CMA)
Cornmeal Dextrose Agar
Potato Flake Agar
Potato Starch Agar
Potato Flake Agar with Yeast
Potato-Carrot Agar
Barley Flour Malt Extract Agar
Barley Flour Modified Sabouraud's
Oatmeal Agar (OA)
Oatmeal Agar A
Oatmeal Agar B
V-8 Oatmeal Agar
V8 Medium
Bean Agar
Faba Bean Dextrose Agar (FDA)
Pea Agar
Cabbage Agar
Dr. Pollock's Modified [Dog Food] Agar

Section D. Other Media and Alternate Formulations
-----------------------------------------------------
Potato-Glucose Agar 1
Potato-Peptone Medium
Potato-Peptone-Yeast Agar (PPYA)
-
Malt Agar (MA)(FDA M185)[aka 2% MEA]
Difco Malt Extract Broth (FDA M94)
Malt Extract Agar for Yeasts and Molds (MEAYM)(FDA M182)
Malt Extract Peptone Agar
Raper & Thom MEA (RTMEA)
ISP 2 Medium (Malt, Yeast, Glucose)
-
Yeast Extract Agar (YEA)(FDA M181)
Yeast Glucose Agar
Glucose and Yeast Extract Agar
Glycerin Yeast Agar
-
Manure Tincture
Manure Agar
-
Starved Agar
Gelatin Agar (GA)(FDA M54)
Plate Count Agar (SMA)(aka Standard Methods Agar)(FDA M124)
Nutrient Agar (FDA M112)
Starch Agar (FDA M143)(Nutrient agar with starch)
Starch-Yeast Agar
1/5 Starch-Yeast Agar
Long-term Preservation Medium (FDA M85)
Peptone Meat Agar (Meat Water)

Section E. Common Solutions
------------------------------
Gentamicin Sulfate Solution (FDA M57)

Section F. Sources
-------------------
List of sources.


The whole thing doesn't seem to want to show up, so grab the text file from the top of the post.
What's your favorite media(s)?
You may choose many
Potato dextrose
Potato dextrose yeast
Malt extract
Karo/sugarwater
Honeywater
Other


Votes accepted from (08/12/04 02:00 AM) to (No end specified)
You must vote before you can view the results of this poll



Edited by fastfred (05/03/06 01:25 AM)


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Invisiblefastfred
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Re: FastFred's Media Cookbook [Re: fastfred]
    #2996265 - 08/12/04 05:34 PM (12 years, 3 months ago)

I decided to post my list of common media recipes. I realize that there are probably similar guides out there. Please post any links, additional recipes, and corrections.

I plan to tidy it up a bit and add an index. Some of the entries are redundant, but I think that's helpful to see the similarities and slight differences. I also need to add some more recipes and find a nutritional analysis for potato infusion. I would like to add DFA, honeywater, and karo also, so if anyone has a favorite recipe or if there is already a consensus on the best karo/honeywater please let me know.


-FF


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OfflineKillBill
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Re: FastFred's Media Cookbook [Re: fastfred]
    #2996414 - 08/12/04 06:17 PM (12 years, 3 months ago)

Well i said Honey Water and Karo cause those are the only two i know how to do now. I can't find malt, i cant' find dextrose, i can find potatoes, but do i really feel like doing any (even minor) amounts of chemistry?
Teaspoon, dump two of whatever into water.. cook... Done.

I would like to try malt though, i hear its fastest (mycopsycho)


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Re: FastFred's Media Cookbook [Re: fastfred]
    #2996461 - 08/12/04 06:32 PM (12 years, 3 months ago)

I use a following mixture as a liquid medium and it works quite well:

16.5 g dextrose
1.5 g yeast
500 ml tap water

Here is a picture of mycelium growing in that broth:



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Re: FastFred's Media Cookbook [Re: fastfred]
    #2996511 - 08/12/04 06:40 PM (12 years, 3 months ago)

Btw.: Have found a huge list of different agar media at dsmz:

http://www.dsmz.de/media/media.htm


Edited by ragadinks (08/12/04 06:46 PM)


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Invisiblemycopsycho
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Re: FastFred's Media Cookbook [Re: fastfred]
    #2996548 - 08/12/04 06:46 PM (12 years, 3 months ago)

we all know what i like......


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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Invisiblefastfred
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Re: FastFred's Media Cookbook [Re: ragadinks]
    #2996585 - 08/12/04 06:53 PM (12 years, 3 months ago)

Thanks for the link.  Most of the info in the cookbook comes from here:
FDA Bacteriological Analytical Manual(BAM) Media Index

I've tried to stick with simple medias that are known to work.

Come up with a title, description, and the source you want listed and I'll add it to the cookbook! :thumbup:


-FF


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Offlineragadinks
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Re: FastFred's Media Cookbook [Re: fastfred]
    #2996754 - 08/12/04 07:29 PM (12 years, 3 months ago)

Here is another good article from www.mushworld.com that examines the influence of different media on the growth of fungi:

Quote:

The morphology and growth rate of over 70 strains of 20 species of edible and medicinal mushrooms from the culture collection of N.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine were studied on 5 agar media. The optimal incubation time, temperature, and suitable media were determined for each culture. One natural and three synthetic liquid media with mineral and organic nitrogen sources were also tested. The effect of medium pH on the mycelial growth was determined by using phosphate buffers with pH values between 3.0 - 8.0. In each stage of inoculum preparation we used physiologically active mycelia. Factors affecting mycelial growth of some medicinal mushrooms in submerged culture were investigated. Inoculum size and composition and nutrient medium pH were important for maximising growth rate and biomass yield and minimising the duration of the lag phase of growth.




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Invisiblefastfred
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Re: FastFred's Media Cookbook [Re: KillBill]
    #2996783 - 08/12/04 07:33 PM (12 years, 3 months ago)

Quote:

KillBill said:
Well i said Honey Water and Karo cause those are the only two i know how to do now. I can't find malt, i cant' find dextrose, i can find potatoes, but do i really feel like doing any (even minor) amounts of chemistry?




Table sugar is sucrose. It is composed of two simpler sugars called glucose(dextrose) and fructose. Glucose is often called dextrose or corn sugar, and is made from cornstarch.

There isn't any chemistry involved in making PD. If you can cook sliced potatos (don't even have to peel them) for 30 min, then you can make PD broth.

Use corn sugar for the dextrose. If you can't find that, just use table sugar (sucrose).


-FF


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Re: FastFred's Media Cookbook [Re: fastfred]
    #2996870 - 08/12/04 07:49 PM (12 years, 3 months ago)

table sugar should be a last resort though.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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Re: FastFred's Media Cookbook [Re: fastfred]
    #3000713 - 08/13/04 02:35 PM (12 years, 3 months ago)

You're killing me here.

Glucose - A monosaccharide sugar, C6H12O6

Dextrose - C6H12O6?H2O -- not a monosaccharide.


Very similar in structure, but not the same.

And while corn syrup may contain dextrose, glucose, and fructose. It is most certainly not a simple sugar.

They are interchangeable, sure. But saying they are the same thing is poor misinformation.


Very nice compilation of medias though. Thank you for the post.


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Re: FastFred's Media Cookbook [Re: mycopsycho]
    #3000746 - 08/13/04 02:43 PM (12 years, 3 months ago)

Quote:

mycopsycho said:
we all know what i like......



Boobies!?!?  :eek:





oh, and malt extract too I guess. :smile:


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Invisiblefastfred
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Re: FastFred's Media Cookbook [Re: Raadt]
    #3001303 - 08/13/04 04:43 PM (12 years, 3 months ago)

Quote:

Raadt said:
Glucose - A monosaccharide sugar, C6H12O6

Dextrose - C6H12O6?H2O -- not a monosaccharide.




Glucose and dextrose monohydrate are the same. There are hydrated and monohydrated forms of both. Check out sigma's website...

Dextrose, USP, anhydrous
Synonyms: D-(+)-Glucose, grape sugar, corn sugar
Molecular Formula: C6H12O6

Dextrose monohydrate, USP
Synonyms: D-(+)-Glucose monohydrate, glucose
Molecular Formula: C6H12O6?H2O

So... table sugar(sucrose) is a mixture of fructose and dextrose(glucose) monohydrate. Corn sugar is dextrose. Dextrose is assumed to be in it's nonhydrated form if not specified.


Quote:

Very similar in structure, but not the same.




I assume that the invivo conversion is trivial to the organism.


Quote:

And while corn syrup may contain dextrose, glucose, and fructose. It is most certainly not a simple sugar.




I don't know about corn syrup, but corn sugar is dextrose(glucose).


Quote:

Very nice compilation of medias though. Thank you for the post.




Thanks Raadt. I plan to HTMLize it at some point, after I've expanded it a bit.


-FF


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Re: FastFred's Media Cookbook [Re: Spudz76]
    #3001677 - 08/13/04 06:16 PM (12 years, 3 months ago)

Quote:

Spudz76 said:
Quote:

mycopsycho said:
we all know what i like......



Boobies!?!?  :eek:
oh, and malt extract too I guess. :smile:



yeah.... i like both of them.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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Re: FastFred's Media Cookbook [Re: mycopsycho]
    #3001732 - 08/13/04 06:39 PM (12 years, 3 months ago)

Malt n Boobs are great!

Wanna try my Sierra Nevada kill your spores tek?


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Re: FastFred's Media Cookbook [Re: PsyllyMe]
    #3001735 - 08/13/04 06:40 PM (12 years, 3 months ago)

lol..... no, my spores have a zest for life.


--------------------
I Am The Sickness.

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Re: FastFred's Media Cookbook [Re: fastfred]
    #3004840 - 08/14/04 06:11 PM (12 years, 3 months ago)

Impressive copy&paste job :shake:

Maybe it would be nice to mention that half of the recipes you give are not intended for the cultivation of mushroom mycelia but for the cultivation of bacteria. Mushroom mycelia will grow very poorly on these media.

For fungi just stick to malt extraxt agar, potato dextrose agar and oatmeal agar and forget about exotic recipes like PCA or Sabouraud


--------------------
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Re: FastFred's Media Cookbook [Re: Una]
    #3009482 - 08/16/04 04:56 PM (12 years, 3 months ago)

Thanks Una. It is indeed a copy&paste job. My intention was to save people some time by collecting this info together. It's much easier than wading through 100s of media types. I'm hoping it will grow into more than just a cut&paste collection as people add their own comments, recipes, and ideas.


>Maybe it would be nice to mention that half of the recipes you give are not intended for the cultivation of mushroom mycelia but for the cultivation of bacteria.<

I just picked out all the media types that are both simple to make and will grow mycelium. Growth speed is not a major factor in petri dish cultivation. Sometimes it's useful to change the media for your specific needs. It's also good to change up the media to keep your mycelium vigorous. Just switching up PDA and MEA is kind of limited.


>For fungi just stick to malt extract agar, potato dextrose agar and oatmeal agar and forget about exotic recipes like PCA or Sabouraud<

From: PML Microbiologicals
"Sabouraud Dextrose (SabDex) Agar is used for the isolation, cultivation, and maintenance of saprophytic and pathogenic yeasts and fungi.

SabDex Agar is an excellent substitute for Malt or Potato Dextrose Agar, when used by mushroom cultivators to propagate mushroom mycelium."

Uses include testing homes, basements, schools, businesses, and vehicles for toxic mold.

Sabouraud Dextrose Agar was described by Sabouraud in 1892 and was used for the identification of fungi based on their morphological characteristics. Sabouraud Dextrose Agar is a standard medium used to support the growth of yeasts and molds. It supplies peptone as the protein source and dextrose as the carbohydrate source for nourishment. Bacterial suppression occurs due to the low pH. This media is especially suited for the primary isolation of fungi from normally sterile sites such as cerebrospinal fluid (CSF).

Later, Emmons modified the medium by decreasing the dextrose content and adjusting the pH closer to the neutral range. This modification enhances sporulation and is particularly useful for the subculture of fungi that so not develop fruiting structures on other media, and so is useful in their identification. It also serves as a good holding medium for stock cultures.

Source: PML Microbiologicals


If you've tried any of the media types in my list an find that they don't work, please post a message or PM me and I'll change the guide.


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Re: FastFred's Media Cookbook [Re: fastfred]
    #3014550 - 08/17/04 06:04 PM (12 years, 3 months ago)



--------------------
To give is to live...



Edited by ATWAR (08/17/04 06:15 PM)


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Re: FastFred's Media Cookbook [Re: ATWAR]
    #3022176 - 08/19/04 07:16 AM (12 years, 3 months ago)

It strange that a lot of professional culture/mycology institutes recommend a much shorter sterilization time then it says in the link you provided:
Quote:

All media have a near-neutral pH and should be sterilized for twenty to thirty minutes at 15 psi pressure.




Here it says 20-30 minutes whereas some site recommend 10-15 minutes:
Here 10 minutes are recommended and here 10 - 15 minutes are recommended and here at the dmsz also 10 minutes are recommended.
I would rather stay with the 10-15 minutes, what do you think ?


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