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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Agar, HEPA Filter, Malt Extract, Potato Dextrose

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Invisiblefrankz00
monkey man

Registered: 04/28/04
Posts: 153
Loc: mad house
Potato Honey Agar
    #3081451 - 09/02/04 07:25 AM (12 years, 3 months ago)

I prepare this variant of the PDA with instant potatoes, honey and agar. Sterilized in the microwave during 3-4 minutes approximately. :wink:

Agar Recipe:
10 grams agar, 5 grams instant potato flakes and 10 ml honey



:microwave:


Edited by frankz00 (09/02/04 07:45 AM)


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Invisiblefrankz00
monkey man

Registered: 04/28/04
Posts: 153
Loc: mad house
Re: Potato Honey Agar [Re: frankz00]
    #3081454 - 09/02/04 07:26 AM (12 years, 3 months ago)

:thumbup:


Edited by frankz00 (09/02/04 07:31 AM)


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Invisiblefrankz00
monkey man

Registered: 04/28/04
Posts: 153
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Ooops! [Re: frankz00]
    #3081461 - 09/02/04 07:30 AM (12 years, 3 months ago)

Fucking contamination!



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Invisiblefastfred
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Re: Ooops! [Re: frankz00]
    #3081509 - 09/02/04 07:53 AM (12 years, 3 months ago)

Why are you adding the honey?

Also 10g of agar will be close to being only semisolid. Do the flakes and honey help it solidify?

Your pics are really fuzzy. The growth looks pretty average... Have you compared it to PDA or MEA?


-FF


Edited by fastfred (09/02/04 09:42 AM)


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Invisiblefrankz00
monkey man

Registered: 04/28/04
Posts: 153
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Re: Ooops! [Re: fastfred]
    #3081540 - 09/02/04 08:10 AM (12 years, 3 months ago)

It?s simple, I don?t have dextrose and I add the honey. Well, I dissolve all the ingredients with boiling water before sterilizing. The best is MEA!  :wink:

What are some good agar media recipes?


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Invisiblefastfred
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Re: Ooops! [Re: frankz00]
    #3081704 - 09/02/04 09:29 AM (12 years, 3 months ago)



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Invisiblefrankz00
monkey man

Registered: 04/28/04
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Re: Ooops! [Re: fastfred]
    #3081720 - 09/02/04 09:41 AM (12 years, 3 months ago)

:eek:
Nice Post! :thumbup:


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InvisibleATWAR
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Re: Ooops! [Re: frankz00]
    #3083320 - 09/02/04 05:05 PM (12 years, 3 months ago)

How much water do you use in that recipe?


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To give is to live...



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Invisiblefrankz00
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Re: Ooops! [Re: ATWAR]
    #3087262 - 09/03/04 02:00 PM (12 years, 3 months ago)

500 ml (something more for evaporation)


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OfflineBaby_Hitler
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Re: Ooops! [Re: fastfred]
    #3098339 - 09/06/04 10:57 AM (12 years, 3 months ago)

10g of agar is plenty solid enough.


I made some potato fructose agar once that did well.

These days I use mostly some dme from the brew store, some agar flakes, and a drop or two of soy sauce for nitrogen.

Plus peroxide, I always use peroxide, usually 6cc liter/quart.


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Offlinetheknighterrant
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Re: Potato Honey Agar [Re: frankz00]
    #3102850 - 09/07/04 12:55 PM (12 years, 3 months ago)

how do you find microwave sterilization? lots of success? would it work for liquid culture (ie: honey tek)? new to cultivation so i am investigating various techniques.

tke


--------------------
The oldest and strongest emotion of man is fear. The oldest and strongest kind of fear is fear of the unknown.
--H.P. Lovecraft

Demented Piper Press


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Invisiblefrankz00
monkey man

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Re: Potato Honey Agar [Re: theknighterrant]
    #3103059 - 09/07/04 02:03 PM (12 years, 3 months ago)

well, the microwave sterilization it?s great and more quick than pc. Also I used microwave to sterilized honey tek, water to make spore syringes... with great results. The rate of contamination is 10% but I don?t have flowhood with hepa filter, I work in glovebox.

I also use peroxide 5-10 cc with great results! :thumbup:


Edited by frankz00 (09/07/04 02:10 PM)


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Offlinetheknighterrant
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Re: Potato Honey Agar [Re: frankz00]
    #3112915 - 09/09/04 12:12 PM (12 years, 3 months ago)

what are your microwaving times for sterilization, jar sizes, etc? interested in using microwave for sterilization, but seems to be little info that i can find. maybe i am looking in the wrong places?

tke


--------------------
The oldest and strongest emotion of man is fear. The oldest and strongest kind of fear is fear of the unknown.
--H.P. Lovecraft

Demented Piper Press


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Invisiblefrankz00
monkey man

Registered: 04/28/04
Posts: 153
Loc: mad house
Re: Potato Honey Agar [Re: theknighterrant]
    #3113061 - 09/09/04 12:55 PM (12 years, 3 months ago)

to sterilize is 3-8 minutes depending the power of microwave.
3-4 minutes to agar recipe or honey tek.

Microwave Sterilization


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Offlinetheknighterrant
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Re: Potato Honey Agar [Re: frankz00]
    #3116732 - 09/10/04 01:39 AM (12 years, 3 months ago)

thanks for the info.

tke


--------------------
The oldest and strongest emotion of man is fear. The oldest and strongest kind of fear is fear of the unknown.
--H.P. Lovecraft

Demented Piper Press


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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Agar, HEPA Filter, Malt Extract, Potato Dextrose

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