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Offlinecommersh
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Registered: 02/14/04
Posts: 3
Last seen: 10 years, 11 months
media carmelization problem, cooker sterilization
    #2426224 - 03/13/04 05:04 AM (13 years, 1 month ago)

i think i have been carmelizing my MEA media when i sterilize it. i have read that this is when the sugars in the media get broken down or something and it renders the media useless for growing mycelium or even bacteria. i have tried to get things to grow on it but nothing seems to be working. the mnedia is a little bit separated when i take it out of the cooker. i have not tried cooking it for less than about 45 mins of 15 psi and i plan to try a shorter cook period. i have a cooker with a valve not a weight so i can keep it from releasing excess steam and making the media boil. however it still seems to boil a little and the spores dont germinate on it after its been poured. is there some way to tell if i have been caramelizing the agar media? has anyone else ever done this? how do i avoid it? the cooker i have is a all american, one of the big ones. i put obout an inch or so of water in the bottom and exhaust air first etc.

comm


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InvisibleJoshua
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Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: media carmelization problem, cooker sterilization [Re: commersh]
    #2426309 - 03/13/04 06:07 AM (13 years, 1 month ago)

I PC my media for about 25 minutes @ 15 lbs. I'm sure if you are doing it for 45 min or longer that time is a huge contibuting factor to the carmelization you are getting.

Joshua


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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