Home | Community | Message Board


Edabea
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Seeds Store Buy CBD, Cannabis Seeds   Amazon Agar, Malt Extract, Petri Dish, Pressure Cooker

Jump to first unread post. Pages: 1
Anonymous

Re: Hello, can I sterilize media filled glass petris in the microwave?
    #91857 - 01/19/00 04:58 PM (17 years, 6 months ago)

I wouldn't reccomend it. Heating agar excessively will cause the sugars to caramalise and this will inhibit growth. Pressure cooking is the best way to go.


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Hello, can I sterilize media filled glass petris in the microwave?
    #91859 - 01/19/00 05:53 PM (17 years, 6 months ago)

Ooh, yeah, pressure cooking. We all know how cool it is in one of those, right?

Glass petris? You lucky...

Yeah. Nukes can do anything pressure cookers can. Do a few bursts of 3 or 4 minutes on full power, then give it a minute to cool. Leave the whole lot in the nuke overnight to cool down. That way, any air that touches the media should be air from the inside of the nuke, and therefore sterile.

Let us know how it does, ne?



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Hello, can I sterilize media filled glass petris in the microwave?
    #91861 - 01/20/00 04:32 AM (17 years, 6 months ago)

MOE dont be so abrupt :frown:

I will try it tonight and will let you guys know. Thanks for the input :wink:

I am a lucky SOB since I got 50 glass petris
two erlenmayer flasks, 2 #11 scalpels w/60 sterile blades and a kg of agar agar for abt $60.



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Hello, can I sterilize media filled glass petris in the microwave?
    #91863 - 01/21/00 03:47 AM (17 years, 6 months ago)

I am glad I had no time to try this yesterday. Your advise let me think of putting the petris inside a tupper...


Watcha think?



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Hello, can I sterilize media filled glass petris in the microwave?
    #91864 - 01/21/00 07:39 AM (17 years, 6 months ago)

Burple, I just noticed your post, sorry I didnt reply before. I did it. What heppened to Moe is prolly becuase he didnt have pyrex or some other heat resistant petri's.
Now, I did a few experiments with this - the major problem I found was over drying of the agar. M/Ws work by sending waves which move the water molecules faster and faster thus causing them to warm up, until vaporization.
This causes the agar to get quite dry.
#1 experiment - I placed a few petris in the M/W and nuked for 8 minutes. Agar became to dry.
#2 experiment - I placed a few petris in the M/W with aa glass of distilled water in the side and nuked for 8 minutes. had a 70% sterlizing succes (maybe this wasnt due to the M/W but to my actions later).
#3 experiment I placed 6 petris inside a rice cooker (its a little plastic pot which goes in the m/w and really heats up, ccoking a large amouint of rice in 20 minutes). I also added a few inches of distilled water in the bottom of the cooker. Nuked for 10 minutes. 100% succes.
#4 experiment -placed a few dishes in the m/w, with a glass of distilled water in the side. Nuked for 4.5 minutes and waited 5 minutes. Nuked for another 4.5 minutes. 100% succes.

Hope this helps.

Later,
tweedy



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Hello, can I sterilize media filled glass petris in the microwave?
    #91865 - 01/21/00 07:51 AM (17 years, 6 months ago)

Tweedy,
More precise you could not be!

!NUKE! here I come

will let you know...



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Hello, can I sterilize media filled glass petris in the microwave?
    #91867 - 01/25/00 05:45 AM (17 years, 5 months ago)

For your Guidance:

Twenty four plates were put in the oven rack empty and "cooked" for 2 hours at 140 centrigrade.

I made up my MEA after boiling the water for 20 mins.

Let the oven cool to 30 Centigrade with the MEA juice cooling with it.

Poured the dishes as soon as everything had cooled so that the agar didn;t cook on contact. Made mistake with one dish initially pouting while too hot.

The petris are incubating at 80 Farheneit.
2 days and no contam germination.

I didn't use the microwave yet I;ll see how this works because one can do many dishes
with the oven serilization tek (if it works)

keeping fingers crossed!

------------------
I burped and she turned purple.....



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Hello, can I sterilize media filled glass petris in the microwave?
    #91868 - 01/26/00 09:45 AM (17 years, 5 months ago)

Good luck burple.
Post results soon ok?

Later,
tweedy.



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Hello, can I sterilize media filled glass petris in the microwave?
    #91869 - 02/08/00 09:20 AM (17 years, 5 months ago)

Well bad news, :frown:

Out of 24 only 5 made it without contams.
I wait on them a while before I use them to make sure.

I'm glad I have an abundance of everything so it works for me this way.

Cleanup is a bitch but what to do.

Personally I am reverting to the old fashion Press. Cooker. Better odds/more hassle but better than cleanup!

BURP

------------------
I burped and she turned purple.....



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Hello, can I sterilize media filled glass petris in the microwave?
    #91870 - 02/08/00 09:35 AM (17 years, 5 months ago)

el hornomicrondas != good...

Check it out, here is the deal.

Without pressure you cannot raise the temp above boiling. Reason is goes down to all those funky equations from science classes:
PV=nRT hehe... I can't believe I remember that.

The base is, it will NEVER get hotter than 100 degrees celcius, it doesn't matter if you set your oven at 140. Because the process of boiling cools the substrate down to 100 till all the water is gone. When the water is gone, it will heat above 100 celcius, but without water the whole damn things pretty useless.

Issue #2 with el microndas:
Duration... microwaves cannot provide the same duration as pcooker. As someone duely noted it dries overly much. The key is having the right temp for the right amount of time.

Oh yea, and make sure to eat some shrooms. Oh, and don't disobey the golden rule of shroom growing:
Don't grow shrooms while on shrooms. At least it never works for me no matter how many times I try it.

Hehe...
later,
ootw.



Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Seeds Store Buy CBD, Cannabis Seeds   Amazon Agar, Malt Extract, Petri Dish, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Where can i find Glass Petri dishes?..anyone? Numba9 1,156 6 09/02/01 04:38 AM
by oscill8
* UV Lamp sterilization? FreeSporePrints 2,356 18 11/15/06 02:08 PM
by agar
* Petri dishes and agar MyOwnReality 2,529 18 03/01/08 10:12 PM
by MycoAu
* Some proven agar nutrient media recipes Una 5,196 11 10/03/04 03:15 PM
by YSr
* Petri Process liveby 860 7 09/25/08 08:34 AM
by denger
* Alternate to petri dishes!! Curious_George 1,325 10 01/03/03 10:34 AM
by mycofile
* Petri dish question German Kahuna 1,620 14 01/10/09 06:33 AM
by German Kahuna
* Plastic Petris'...am i screwed?
( 1 2 all )
Azmodeus 1,903 20 02/07/03 11:35 PM
by stonErollEr1

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, bodhisatta
1,373 topic views. 0 members, 3 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Phytoextractum
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.006 seconds on 17 queries.