Home | Community | Message Board


Azarius
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Agar, Propolis, Scales

Jump to first unread post. Pages: 1
OfflineMindzpore
Psychedelicious
 User Gallery

Registered: 04/05/05
Posts: 319
Loc: Reject the concept of loc...
Last seen: 10 months, 16 days
various liquid culture media
    #4274879 - 06/09/05 06:13 AM (12 years, 2 months ago)

I'm thinking to do some trials of different LC's. why? well, i personally find it interesting, and i think it might be of benefit with a side by side comparison of various types of media.

also, with my current situation/space this is about as ambitious a project as can be done.

my plan is to use around 45 small glass jars (250ml). using three jars for each media. this will allow me to compare 15 types of media.

they will all be nocced at the same time, with the same amount of the same monoculture from another LC. and resulting growth recorded (with pictures) on a regular basis.

Now, it will be at least a month and a half before i can really start this. but untill then i would appreciate any kind of input and suggestions. especially i would like suggestions on what type of media you think should be included (your favourites, or crazy/creative ideas). and also of course on method.

Mz


--------------------
Mindzpores words of wisdom:
"If you think something is foolproof, you just haven't met proper fools".

Wiccan_Seeker said:
"It is better to adjust to become a better listener than to keep on cranking up the volume".


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineblackout
Microwaver
 User Gallery

Registered: 07/17/00
Posts: 4,875
Last seen: 18 hours, 17 minutes
Trusted Cultivator
Re: various liquid culture media [Re: Mindzpore]
    #4274891 - 06/09/05 06:21 AM (12 years, 2 months ago)

I think they should all have the same sugar solution, calculated correctly, i.e. honey is not 100% sugar, I don't think karo is either. Identical jars, heat, filters etc.

I would like to see simple stuff readily available rather than fancy expensive or inaccessible stuff like peptone, bee pollen, vanilla or elephant dung!

Definitely coffee, apple juice. Maybe non sugar teas with added glucose/dextrose, like manure or straw tea. Or water vegetables were boiled in.


--------------------
support the free spore ring http://fsre.nl/


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMindzpore
Psychedelicious
 User Gallery

Registered: 04/05/05
Posts: 319
Loc: Reject the concept of loc...
Last seen: 10 months, 16 days
Re: various liquid culture media [Re: blackout]
    #4275054 - 06/09/05 08:59 AM (12 years, 2 months ago)

i could even do it several times, using different strains or speices. that should make clear what solutons suit what type better.


--------------------
Mindzpores words of wisdom:
"If you think something is foolproof, you just haven't met proper fools".

Wiccan_Seeker said:
"It is better to adjust to become a better listener than to keep on cranking up the volume".


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMindzpore
Psychedelicious
 User Gallery

Registered: 04/05/05
Posts: 319
Loc: Reject the concept of loc...
Last seen: 10 months, 16 days
Re: various liquid culture media [Re: Mindzpore]
    #4278686 - 06/10/05 04:55 AM (12 years, 2 months ago)

what? no suggestions?


--------------------
Mindzpores words of wisdom:
"If you think something is foolproof, you just haven't met proper fools".

Wiccan_Seeker said:
"It is better to adjust to become a better listener than to keep on cranking up the volume".


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinecaptmueller
chemical shaman
Registered: 10/25/04
Posts: 115
Last seen: 10 years, 6 months
Re: various liquid culture media [Re: Mindzpore]
    #4278726 - 06/10/05 05:14 AM (12 years, 2 months ago)

i have an experiment with some potato water from making agar mixed with karo water incubating now, i suppose we will see in a few days...

namaste,
captmueller


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePinback
Stranger
 User Gallery

Registered: 07/20/02
Posts: 784
Loc: Northern Europe
Re: various liquid culture media [Re: Mindzpore]
    #4278932 - 06/10/05 08:52 AM (12 years, 2 months ago)

How do you plan on quantifying the growth?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepsilocyben
bad guy

Registered: 12/03/04
Posts: 1,026
Last seen: 10 years, 7 months
Re: various liquid culture media [Re: Pinback]
    #4279210 - 06/10/05 11:34 AM (12 years, 2 months ago)



--------------------




Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMindzpore
Psychedelicious
 User Gallery

Registered: 04/05/05
Posts: 319
Loc: Reject the concept of loc...
Last seen: 10 months, 16 days
Re: various liquid culture media [Re: Pinback]
    #4290191 - 06/13/05 06:17 AM (12 years, 2 months ago)

Quote:

Pinback said:
How do you plan on quantifying the growth?




well, at first i was thinking to filter, dry and weigh the stuff... but then... i just relaxed. the idea is to see if/what media gives quicker growth. and if the difference is just a few 0.x grams... its completely irrelevant. so ill just go with visual inspection. and pictures. if i cant see a difference in growth speed. it doesnt matter.

another factor that originally interested me was to see if different types of media tended to trigger different types of mycelia development. however, this will obviously be easier to see on an agar plate and will have to wait for a later experiment.


--------------------
Mindzpores words of wisdom:
"If you think something is foolproof, you just haven't met proper fools".

Wiccan_Seeker said:
"It is better to adjust to become a better listener than to keep on cranking up the volume".


Post Extras: Print Post  Remind Me! Notify Moderator
Offline_OttO_
Over Stimulated
 User Gallery

Registered: 06/01/05
Posts: 2,523
Loc: Up Over
Last seen: 12 hours, 30 minutes
Trusted Cultivator
Re: various liquid culture media [Re: Mindzpore]
    #4540530 - 08/16/05 01:27 AM (12 years, 8 days ago)

I have a bag of dry, powdered apple tea which when mixed with hot water dissolves completely. Bought in a small village on the south coast of Turkey - the guy says he makes it himself, and unlike the 'commercially' available brands they have everywhere in Turkey that are mainly sugar, he says his homemade stuff is 90% apples. I think this is going to be promising.....


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHuckleBones
Danger Bones

Registered: 06/16/05
Posts: 385
Last seen: 2 years, 11 months
Re: various liquid culture media [Re: _OttO_]
    #4540644 - 08/16/05 02:02 AM (12 years, 8 days ago)

I've been really meaning to try semen.


I suppose I'll eat a lot of fruit sugars and other things and hopefully my semen will have enough nutes.


--------------------
Manitou


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHotnuts
old hand
 User Gallery

Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 27 days, 21 hours
Re: various liquid culture media [Re: Mindzpore]
    #4540700 - 08/16/05 02:20 AM (12 years, 8 days ago)

Pure vanilla extract..................


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinegrimR
hippiousmaximous
Male User Gallery

Registered: 03/29/06
Posts: 1,235
Loc: North America
Last seen: 1 year, 5 months
Re: various liquid culture media [Re: Hotnuts]
    #5534604 - 04/19/06 08:03 PM (11 years, 4 months ago)

pure vanilla extract works? I thought that had a high alcohol content, you sure its mycellium?


--------------------
- grimR


-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
http://egolost.com 
"I am already given to the power that rules my fate. And I cling to nothing, so I will have nothing to defend. I have no thoughts, so I will see. I fear nothing, so I will remember myself."

- Don Juan teachings


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineFeelers
Anti-Myth-Rhythm-Rock-Shocker
Male User Gallery

Registered: 06/19/02
Posts: 1,806
Loc: Land of Oz
Last seen: 1 month, 5 days
Re: various liquid culture media [Re: grimR]
    #5535699 - 04/19/06 11:56 PM (11 years, 4 months ago)

Yeah man in the vanilla extract I've seen its 50% alcohol, so I'm geussing that ones a different type???


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSnaggletooth
Stranger in a Strange Land
Male User Gallery

Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 3 months, 22 days
Re: various liquid culture media [Re: Feelers]
    #5535722 - 04/20/06 12:00 AM (11 years, 4 months ago)

when you PC it I believe the alcohol 'burns' off....


I hear it works nicely...


--------------------


Atheist Chat


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinecampinman
Fun-Guy
Male User Gallery

Registered: 09/01/05
Posts: 398
Loc: SoOn
Last seen: 9 months, 30 days
Re: various liquid culture media [Re: Snaggletooth]
    #5537869 - 04/20/06 04:01 PM (11 years, 4 months ago)

i was thinking about trying out some vanilla the other day, got the same old crown karo again though.


id really like to see someone do a test with lots of different mediums, try and see what goes quickest, or strongest, or thickest, or whatever. cool idea man, i think alot of folks could learn from a nicely setup large-scale exp.


cheers


Edited by campinman (04/20/06 04:07 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinealsnow469
Stranger

Registered: 10/15/05
Posts: 389
Last seen: 12 hours, 39 minutes
Re: various liquid culture media [Re: HuckleBones]
    #5575889 - 05/01/06 01:34 AM (11 years, 3 months ago)

Yours's or someone else's?


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: various liquid culture media [Re: alsnow469]
    #5584597 - 05/03/06 08:30 AM (11 years, 3 months ago)
Log in to view attachment

Vanilla extract = pure foolishness.

Here is a cookbook of various standardized media types. Just omit the agar for liquid media. See the attached file. I would be quite interested to see the results if you have the time to test some of these wacky recipes.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Agar, Propolis, Scales

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* can liquid culture be done with sucrose? bobmarley4prez 1,723 13 12/27/03 09:58 AM
by ragadinks
* Compost Tea Liquid Culture SoopaX 4,260 19 05/23/05 11:44 PM
by VALIS
* liquid culture to peroxyde grains...?! fredyjenkins 1,510 5 08/28/05 02:49 AM
by mycofile
* Strange liquid culture results? Shdwstr 1,732 3 01/15/02 01:37 AM
by fresh357
* Agar Culture transfer to Liquid Culture Tek CompilerV 6,614 11 06/27/03 07:11 PM
by Compiler
* liquid culture to sawdust
( 1 2 all )
rungi 6,393 29 12/04/08 01:24 AM
by lipa
* liquid culture
( 1 2 all )
houdinihar 5,750 28 03/01/09 03:38 PM
by fastfred
* Ghetto liquid culture methods
( 1 2 all )
b3jamboree 5,958 21 06/01/12 08:38 AM
by OICU812

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, bodhisatta
2,697 topic views. 0 members, 7 guests and 1 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Magic-Mushrooms-Shop.com
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.004 seconds on 16 queries.