Mark_M said:

Mark_M said:

PinePalace said:

Mark_M said:

First time grower. Grain bag… Is this getting close to fruiting time? Or how to know? Thanks!

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See how the mycelium is clumping & has tiny tiny little dots?
Those are called “hyphal knots” & “primordia” which form after full colonization and signal the beginnings of fruiting

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So I did go ahead and cut the top of the bag open a few weeks ago now (19 days). The top area was not fully colonized but quickly did over the next four or five days. Now it looks like this, which I'm imagining is like about ready to fruit? What's the longest after letting it have some air that I would expect fruiting to start? I open it a couple of times a day for 30-40 minutes, then clip it loosely shut the rest of the time.

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Also, I've kept this at 75 degrees - should I be lowering the temp?

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Any thoughts, yes/no/other, much appreciated! Thanks... -Mark

Tri said:

Fiet said:

GenesisCorrupted said:

You don’t trigger fruiting conditions.
It should have already been in fruiting conditions.
Stop depriving the psyllium of oxygen they need to breathe.

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I do not understand I open the Bag 1 time a day so why they can't breath?

What i need too do?

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https://www.shroomery.org/forums/showflat.php/Number/27280275#27280275

read this Fiet

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Mark_M said:

I don't think it's a common problem but doesn't sound like something you'd want...

https://pmc.ncbi.nlm.nih.gov/articles/PMC85751/#:~:text=in%20immunosuppressed%20patients.-,Trichoderma%20spp.,10%2C%2012%2C%2013).
"Opportunistic fungal infections have occurred with increasing frequency in recent years in immunosuppressed patients. Trichoderma spp. are fungi distributed worldwide which rarely infect humans but can cause from localized infections to fatal disseminated disease (5, 6, 8, 10, 12, 13). In this report, we describe a systemic T. harzianum infection in a renal transplant patient which was detected in the necropsy study. The fungus was recovered from abscesses in brain and lung tissues."

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Well shit, if I ever need a renal transplant I'll be sure to leave my trich infested tubs at home, thanks for the heads up.

Joking aside, I would never ingest or breath in any type of mold infested anything on purpose, but it's not like I throw away my firewood because there's som trich on it either.

Throwaway said:

So a some of you guys know I'm a total LC lover and I'm using the Faht steam pen to keep everything sterile.

I'm gonna go for a filterless LC for the first time - mainly thinking about long term storage and to expand from that one endlessly. So I'll just be going with an injection port and gonna keep it in the fridge to keep a culture for (hopefully) years and years.

Now the question;

If I want draw some of the culture up, to avoid the vaccuum... would it be wise to squirt in the same amount of sterile water you guys think? I can just fill a 20ml syringe with water and PC it, put it into the LC and push the plunger to build up some pressure and just suck 20ml of LC back up?

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Throwaway said:

So a some of you guys know I'm a total LC lover and I'm using the Faht steam pen to keep everything sterile.

I'm gonna go for a filterless LC for the first time - mainly thinking about long term storage and to expand from that one endlessly. So I'll just be going with an injection port and gonna keep it in the fridge to keep a culture for (hopefully) years and years.

Now the question;

If I want draw some of the culture up, to avoid the vaccuum... would it be wise to squirt in the same amount of sterile water you guys think? I can just fill a 20ml syringe with water and PC it, put it into the LC and push the plunger to build up some pressure and just suck 20ml of LC back up?

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cybork said:

I guess you can do that, but personally I'm a bit paranoid of putting anything in that isn't needed per se.

That said, I do something similar with a syringe+ptfe filter that's PC-ed. I let it suck air in after the LC cooled down by plunging it in the injection port, to get rid of the vaccuum. Haven't got any contamination with it yet (about 15 jars further now).

But maybe that is the steam pen you're talking about. Couldn't find that yet.

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Yeah I've read things about people stuffing a syringe full of polyfill, PCing it and then using that to equalize the pressure. Anyways I'll let you fellas know how it goes

I got the cult bug again after my last grow that went so successful!

Here's a link to the steam pen

Throwaway said:

cybork said:

I guess you can do that, but personally I'm a bit paranoid of putting anything in that isn't needed per se.

That said, I do something similar with a syringe+ptfe filter that's PC-ed. I let it suck air in after the LC cooled down by plunging it in the injection port, to get rid of the vaccuum. Haven't got any contamination with it yet (about 15 jars further now).

But maybe that is the steam pen you're talking about. Couldn't find that yet.

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Yeah I've read things about people stuffing a syringe full of polyfill, PCing it and then using that to equalize the pressure. Anyways I'll let you fellas know how it goes

I got the cult bug again after my last grow that went so successful!

Here's a link to the steam pen

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Two pics to clarify:
The set up:





The ready to grab PC-ed in advance in aluminium foil: (heat needle before use to be safe) edited:*(I PC them by the way, because my filters are cheap and not sterile out of the container)*:


And thanks for the link to the steam pen.

Jebi said:

how do I do better?

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fahtster said:

Tri said:

Fiet said:

I do not understand I open the Bag 1 time a day so why they can't breath?

What i need too do?

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https://www.shroomery.org/forums/showflat.php/Number/27280275#27280275

read this Fiet

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They’ve been directed to this so many times in the last couple months lol.  I threw my hand up a while ago

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Fiet, wenn du willst, dass die Mitglieder hier weiterhin deine Fragen beantworten und dich nicht einfach ignorieren, dann MUSST du aufhören, SIE zu ignorieren! Deine Fragen wurden schon mehrfach beantwortet – du musst den Leitfaden lesen, auf den dich alle immer wieder verweisen. 

Dieser Leitfaden beantwortet alle Fragen, die du gestellt hast. Fahts Ziploc-Fruiting-Chamber-Anleitung erklärt die Schritte – LIES SIE. Du kannst nicht erwarten, dass die Leute dir immer wieder alles vorkauen, wenn du nicht zuhörst und ständig dieselben Fragen stellst. 

Diese Community ist großartig, und viele der Trusted Cultivators nehmen sich die Zeit, einfache Fragen zu beantworten, obwohl der Fragesteller sie auch selbst nachschlagen könnte. Wenn du immer wieder dieselben grundlegenden Fragen stellst, für die du längst Antworten bekommen hast, wirkt es, als wäre es dir egal. Verschwende nicht ihre Zeit, Mann – werde ernsthaft.

Smellyhobbit said:

Myndful said:

What's the best protocol for working with plate pins?

Do I wait for the plate pins to grow out and transfer?

Or work with the small fruit to another agar plate?

Or drop the plate pins to grain?

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I take half the pin and put it to a new plate. The other half goes wherever I want. Usually LC, but sometimes grain. That works great. Taking half to a dish allows you to save it as is just in case it’s super awesome.

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LewDoja said:

Wow Tri never seen this side of you. But I think a lot of it may be a language barrier as you've obviously assumed as well.

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Sorry

I am not multi-lingual but I am an old grandma at heart, I WILL scold you in your native tongue if you’re getting up to shenanigans though what I wrote to fiet is not really scolding rather stern guidance

As far as how well stuff translates- if you use google translate it seems to be pretty poor

Using AI language models allow you to actually communicate in whatever language pretty fluently it seems. It’s what got me through to the German miraculix doctor too.

I just make sure to have it translate the language back and forth at least once to double check and it’s good

Puts out translations people can actually understand lol

PuffM4gicDr4gon said:

I noticed a huge drop in bacteria biofilm when I started using disposable tyvek sleeves. I tuck them into my gloves so there isn't any exposed skin inside the sab.

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This could be a doubled edge sword... I used to use sleeves religiously.

I have dramatically decreased fungal and bacterial contamination by not using sleeves. although the tyvek sleeves are better than any other type of sleeves, due to their lint free characteristics. Maybe my arm holes are just a tad too small for their efficacy.

My newest approach (albeit it's not that recent) is to "scrub" similarly as a you would see a surgeon on TV do...

Soap molecules are amphiphilic, meaning they have both water-attracting (hydrophilic) and oil-attracting (hydrophobic) properties. This allows them to break down the oils and dirt on the skin where microbes reside

Encapsulation of Microbes – When lathered, soap forms micelles—tiny spherical structures that trap oils, dirt, and microbes, suspending them in water.

Rinsing Away Contaminants – Once microbes and debris are encapsulated, they can be easily washed away with water, significantly reducing microbial load.

Mechanical action- facilitating the microbial removal by using a soft nail brush to "scrub" .

Many essential oils have antimicrobial properties as well. Such as Tea Tree or Peppermint, which are often found in common soaps, or can be added to.

The key is to maximize exposure to the suds and mechanical action.