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Re: The Official Wild Cubensis Thread [Re: Huskies]
    #29097032 -

๐Ÿ˜‚

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Re: The Official Wild Cubensis Thread [Re: 3.A.M]
    #29097361 -

:camping:


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๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Re: The Official Wild Cubensis Thread [Re: Pnin] * 1
    #29097458 -

Really happy for the community engagement!

I'll add an edit to the post, but I think it would be worth discussing some commercial strains that are more recent landrace strains and thus almost certainly have a wide genetic profile compared to old school classic cubes.

Been researching around, trusted vendors and others. What newer commercial strains that are out there that are most likely still quite genetically distinct and varied?

"Recent" Wilder Genes that seem to be telling the truth:

Sibu (2023-24) - Costa Rica
Taman Negara (2023) - Malaysian
Tosohatchee (2020) - Central Florida


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: The Official Wild Cubensis Thread [Re: Huskies]
    #29097827 -

I need to reach out to some of my local resources to see if they have any wild prints on hand. I will probably wait until I have an actual MS grow that works out before diving in, but I'm very interested in taking part in this wild spore goodness!


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"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: The Official Wild Cubensis Thread [Re: Huskies] * 3
    #29097909 -

One thing that excites me is that we still don't know the original habitat for Cubes. They grow all over the world, but it's probably the same story as the Australian; a small group of spores found their way to a new habitat, and something of a "founder effect" occurred. The wild cubes of Australia are much more diverse than our cultivated cubes, but the ones in the wild from the original habitat would probably be SUPER diverse. Hopefully as getting genetic sequences gets cheaper, we get a clearer picture as to where the diversity hotspots are.


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:3 ^_^ โ“›ฯ‰โ“› >_< (:  iโ€™ve taken a microbiology class so i must know what im doing!

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Re: The Official Wild Cubensis Thread [Re: malvaneglecta] * 7
    #29097974 -

I think the community should on some level prioritize protecting genetic diversity, especially since climate change guarantees wild specimens will get less and less diverse. Countries use seed banks to preserve wild genetics so that they can always breed better crops, so it makes sense to have some sort of decentralized version of that on the market place here.

Ideally we would have people that just keep well labeled spore-prints in their fridge, but that leaves the problem of distribution. Everyone on this forum is interested in one thing: growing mushrooms. The tragedy is that every time we go from spore-print to spore-print :mushroomgrow:, genetic diversity is drastically reduced. Realistically it takes only 5-6 generations for a culture to be stabilized, which means many wild genetics are lost forever after that. Everyone from noobs to super-producers is probably working with inbreds because thatโ€™s what happens without actively seeking out diverse spores. We arenโ€™t gonna change how the hobby works. 

The way I see it, a single wild spore print should be grown to fruit, and then several of those resulting spore prints should be mailed out. In this way, no one specific person has to try and preserve the diversity. Even if you spore to spore this print several times, it will be different from someone else who received a print from the same person. That way when people are looking to make unique crosses, thereโ€™s a high chance that two people actually have spores with unique genetics.

As fun as it is to chase those perfect monotub genetics, there should also be praise given to people who are keeping a unique culture alive, even if itโ€™s shittier than everyone else's. This is an easy way to keep mushrooms available to everyone for a long time.

Sorry for the wall of text, and especially sorry if people are already doing that in the marketplace, since I havenโ€™t had the opportunity to read that, but I love plant breeding and love when I can apply that stuff to mushroom breeding. :lol:


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:3 ^_^ โ“›ฯ‰โ“› >_< (:  iโ€™ve taken a microbiology class so i must know what im doing!

Edited by malvaneglecta (01/22/25 05:44 PM)

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Re: The Official Wild Cubensis Thread [Re: Huskies]
    #29098029 -

Very cool thread! I'll be monitoring it, even if I can't contribute much to it atm. Been getting into Mushroom Hunting recently, though, and I might take a more serious crack at finding wild patches when it starts raining consistently again.

If money/time allows, I was hoping to sneak a vacation later this year... with this thread in mind, maybe it would be fun to try a bigger Mushroom Hunting spot than I can usually roam in. Veracruz comes to mind... Best case scenario, I could make some interesting contributions to the thread then!

For now, though, I'll just read along and :camping: lurk a little :smile:


--------------------
There is no season when you are grown
You are always risen from the seeds you've sown
There is no reason to rise alone
Other stories given have sages of their own

Greene's LAGM 2025 Log!
Current Projects![/url]

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Re: The Official Wild Cubensis Thread [Re: malvaneglecta] * 5
    #29098074 -

malvaneglecta said:
I think the community should on some level prioritize protecting genetic diversity, especially since climate change guarantees wild specimens will get less and less diverse. Countries use seed banks to preserve wild genetics so that they can always breed better crops, so it makes sense to have some sort of decentralized version of that on the market place here.

Ideally we would have people that just keep well labeled spore-prints in their fridge, but that leaves the problem of distribution. Everyone on this forum is interested in one thing: growing mushrooms. The tragedy is that every time we go from spore-print to spore-print :mushroomgrow:, genetic diversity is drastically reduced. Realistically it takes only 5-6 generations for a culture to be stabilized, which means many wild genetics are lost forever after that. Everyone from noobs to super-producers is probably working with inbreds because thatโ€™s what happens without actively seeking out diverse spores. We arenโ€™t gonna change how the hobby works. 

The way I see it, a single wild spore print should be grown to fruit, and then several of those resulting spore prints should be mailed out. In this way, no one specific person has to try and preserve the diversity. Even if you spore to spore this print several times, it will be different from someone else who received a print from the same person. That way when people are looking to make unique crosses, thereโ€™s a high chance that two people actually have spores with unique genetics.

As fun as it is to chase those perfect monotub genetics, there should also be praise given to people who are keeping a unique culture alive, even if itโ€™s shittier than everyone else's. This is an easy way to keep mushrooms available to everyone for a long time.

Sorry for the wall of text, and especially sorry if people are already doing that in the marketplace, since I havenโ€™t had the opportunity to read that, but I love plant breeding and love when I can apply that stuff to mushroom breeding. :lol:



Well that s my plan! I think these would be the steps:

1- Put some spores and agar, then make a syringe from the rest.
2- Use spore solution to inoculate PF TEK cakes.
3- Grow out the wild genes on cakes, and grab multiple prints from them, send them to people.
4- Without isolating too much from the agar plate, putting a T1 or T2 agar plate to grains, spawn those in shoeboxes.
5- Compare the pure wild vs agar isolate group in terms of potency, yield and phenotypes, and of course grab more prints.

Another thing I want to do is mix wild varieties between each other, like continents and centuries apart (Australia and America), and then mix those with commercial strains to revitalize their genepool.

Either way, its looking like the community might rally around this effort. Could be we come across some interesting new discoveries!

malvaneglecta said:
One thing that excites me is that we still don't know the original habitat for Cubes. They grow all over the world, but it's probably the same story as the Australian; a small group of spores found their way to a new habitat, and something of a "founder effect" occurred. The wild cubes of Australia are much more diverse than our cultivated cubes, but the ones in the wild from the original habitat would probably be SUPER diverse. Hopefully as getting genetic sequences gets cheaper, we get a clearer picture as to where the diversity hotspots are.




I think the recentish discoveries of Natalensis and Ochras in South Africa, both cousin species to cubensis to the point of being able to be cross together, indicates that the of cubensis is probably African in origin.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Edited by Huskies (01/22/25 06:58 PM)

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Re: The Official Wild Cubensis Thread [Re: Huskies] * 2
    #29098225 -

Huskies said:
malvaneglecta said:
I think the community should on some level prioritize protecting genetic diversity, especially since climate change guarantees wild specimens will get less and less diverse. Countries use seed banks to preserve wild genetics so that they can always breed better crops, so it makes sense to have some sort of decentralized version of that on the market place here.

Ideally we would have people that just keep well labeled spore-prints in their fridge, but that leaves the problem of distribution. Everyone on this forum is interested in one thing: growing mushrooms. The tragedy is that every time we go from spore-print to spore-print :mushroomgrow:, genetic diversity is drastically reduced. Realistically it takes only 5-6 generations for a culture to be stabilized, which means many wild genetics are lost forever after that. Everyone from noobs to super-producers is probably working with inbreds because thatโ€™s what happens without actively seeking out diverse spores. We arenโ€™t gonna change how the hobby works. 

The way I see it, a single wild spore print should be grown to fruit, and then several of those resulting spore prints should be mailed out. In this way, no one specific person has to try and preserve the diversity. Even if you spore to spore this print several times, it will be different from someone else who received a print from the same person. That way when people are looking to make unique crosses, thereโ€™s a high chance that two people actually have spores with unique genetics.

As fun as it is to chase those perfect monotub genetics, there should also be praise given to people who are keeping a unique culture alive, even if itโ€™s shittier than everyone else's. This is an easy way to keep mushrooms available to everyone for a long time.

Sorry for the wall of text, and especially sorry if people are already doing that in the marketplace, since I havenโ€™t had the opportunity to read that, but I love plant breeding and love when I can apply that stuff to mushroom breeding. :lol:



Well that s my plan! I think these would be the steps:

1- Put some spores and agar, then make a syringe from the rest.
2- Use spore solution to inoculate PF TEK cakes.
3- Grow out the wild genes on cakes, and grab multiple prints from them, send them to people.
4- Without isolating too much from the agar plate, putting a T1 or T2 agar plate to grains, spawn those in shoeboxes.
5- Compare the pure wild vs agar isolate group in terms of potency, yield and phenotypes, and of course grab more prints.

Another thing I want to do is mix wild varieties between each other, like continents and centuries apart (Australia and America), and then mix those with commercial strains to revitalize their genepool.

Either way, its looking like the community might rally around this effort. Could be we come across some interesting new discoveries!

malvaneglecta said:
One thing that excites me is that we still don't know the original habitat for Cubes. They grow all over the world, but it's probably the same story as the Australian; a small group of spores found their way to a new habitat, and something of a "founder effect" occurred. The wild cubes of Australia are much more diverse than our cultivated cubes, but the ones in the wild from the original habitat would probably be SUPER diverse. Hopefully as getting genetic sequences gets cheaper, we get a clearer picture as to where the diversity hotspots are.




I think the recentish discoveries of Natalensis and Ochras in South Africa, both cousin species to cubensis to the point of being able to be cross together, indicates that the of cubensis is probably African in origin.



Iโ€™m on board to do this too. I have a project that will diversify genetics and I will be spreading the fruits of my labor. I read that paper a few weeks ago and it inspired me to get this going. Iโ€™ll also document my project in this thread. :elmo:

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Re: The Official Wild Cubensis Thread [Re: Teonac] * 1
    #29099382 -

Wow this is a great thread n I'd be honored n very happy to contribute to it too.

So I have a friend who stays out in Texas by Houston who collected a few wild Cubes located near a pasture in that area. He sent me a print he made from that wild collection in 2020 but it wasn't until last spring and summer of 2024 that I got around to taking it out of cold storage, germinating it on agar n fruiting it

Once fruiting it, it gave out some beautiful fruits almost looking like the collected specimen my friend got. I don't have the picture of the wild Cube he sent me but I just asked him if he still had pictures of it and so if he does I'd love to share that. I'll definitely share the indoor tubs tho. I'd like however go back and make a syringe of it from my one T0 plate and spawn it to PF TEK cakes like Huskies said. I did take a few dozen prints tho from the 1st grows.

I ended up calling it Psilocybe Cubensis Houston, Texas since that's where it came from. I know there's a supposed Texas floating around that's just named Texas, but that one has a GT look to it more than these Houstons

Edited by CosmicCamote (01/23/25 06:59 PM)

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Re: The Official Wild Cubensis Thread [Re: CosmicCamote] * 9
    #29099389 -






Ps. Cubensis Houston, Texas ๐Ÿ‡จ๐Ÿ‡ฑ
3 different bins and a handful of 2nd flush fruits. May 2024

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Re: The Official Wild Cubensis Thread [Re: CosmicCamote] * 3
    #29099394 -



Then another bin of them in July 2024. From the same T0 plate

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Re: The Official Wild Cubensis Thread [Re: CosmicCamote] * 7
    #29099502 -

Well it just so happens I recently finally ran my wild print to fruit and have several questionably clean prints. I have it documented from find -> present day information here.

I'm still deciding on what way to go about releasing the spores to the community. My original plan was to pick a pheno and work my way to stabilizing it, then doing a marketplace giveaway.





I have about 5 or so tubs of F-1 actively fruiting and a bunch of F-2 in grain colonizing for my LAGM '25.

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Re: The Official Wild Cubensis Thread [Re: Axman]
    #29099521 -

:threadmonitor:


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Re: The Official Wild Cubensis Thread [Re: Axman] * 2
    #29099878 -

Axman said:
Well it just so happens I recently finally ran my wild print to fruit and have several questionably clean prints. I have it documented from find -> present day information here.

I'm still deciding on what way to go about releasing the spores to the community. My original plan was to pick a pheno and work my way to stabilizing it, then doing a marketplace giveaway.





I have about 5 or so tubs of F-1 actively fruiting and a bunch of F-2 in grain colonizing for my LAGM '25.



I think that'd be a great idea on stabilizing a pheno you'd like then shooting the prints out. That way you not only know what that domesticated lineage is capable of and stable but as well knowing you have clean prints. I felt with the Houstons I got lucky on my end to have a clean print from the wild

I'd also like to look into LAGM'25. I have lots of prints and swabs I could give out <:]

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Re: The Official Wild Cubensis Thread [Re: Axman] * 1
    #29099884 -

That was also a great journal log that I just got around to checkin out. Those pics of the wild specimens are badass n that jar grow is looking good ๐Ÿ‘๐Ÿผ

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Re: The Official Wild Cubensis Thread [Re: CosmicCamote] * 2
    #29100043 -

@CosmicCamote: Nice!! Calling it Houston definitely makes sense, we should aim to be more local in cataloguing these new wild strains. County names in the USA are a good in between. How was the trip of of those?

@Axman: Woot! I am not so sure how much we should worry about stabilization vs providing a wide gamut of genes to do crosses with, but great work!

Has anyone actually done a cross of a wild (f1 or f2) print with a commercial strain?

I understand that wild genes might have a bunch of variables in potency/yield that make them discouraging compared to a commercial one, but I'm very interested in seeing what happens when that gene pool gets revitalized. Maybe its best of both worlds?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: The Official Wild Cubensis Thread [Re: Huskies]
    #29100075 -

I would like to get mine sequenced, but have no idea where to even start with that. Can I just mail some spores somewhere or does it need to be fresh tissue? Ive done some reading on it and I'm still lost.

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Re: The Official Wild Cubensis Thread [Re: Axman]
    #29100106 -

Tissue would be better than spores. Dunno about mushrooms in particular, but fresh is best, or frozen in nitrogen. Dried is likely ok as long as it hasn't been too long or exposed to a lot of heat. But I have no specific knowledge about mushrooms.

They can sequence preserved, museum specimens that are centuries old. It might not be cheap, but it can be done. Spores wouldn't be the best because you'd like a complete genome, but still doable. However, a pristine sample is likely to be the easiest and cheapest to work with.

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Re: The Official Wild Cubensis Thread [Re: Huskies]
    #29100142 -

Huskies said:
I understand that wild genes might have a bunch of variables in potency/yield that make them discouraging compared to a commercial one, but I'm very interested in seeing what happens when that gene pool gets revitalized. Maybe its best of both worlds?



I feel like the goal of genetic variety and cultivation just not might go hand in hand. Even if one were to find something interesting in a much wider gene pool, for cultivation purposes it would make sense to then still select towards it.

One thing that I immediately thought were wild apple trees. Large majority of the time they are significantly worse than tasty and large commercial cultivars and I can't imagine lots of effort going towards finding something novel from them. Though I admit, it's apples and oranges, considering the length of time apples are domesticated vs mushrooms.

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