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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9] * 1
    #29097928 -

They make these little rubber mats to line shelves with, so glasses & dishes don't slide around. It comes rolled up like aluminum foil and can be cut to length. That would be my plan. Easy to clean and hygienic as well. Store it right in your SAB when not in use. 👍🏻

I found it serendipitously and use it in my glovebox; not only does it prevent my plates from sliding around but, more, importantly, it's black so I can see what I'm doing better. The bottom of my glove box is white & I couldn't see shit with the contrast. 🤣


--------------------
Chock full o' nuts. ☕

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9]
    #29097933 -

KlarnEvil9 said:
QQ: What should I put on the table that has my SAB on it to prevent slipping? It's a folding plastic table, pretty slick on top without a towel or something.

I know that a bath towel is probably not the way, but I'm not sure what I should be looking for. A different type of towel? Or some adhesive rubber gasketing around the lip that makes contact with the table?

Current SAB pic:


She's a sight better than the soft sided one I had (I know I suck), but I don't know if the towel is causing problems or helping solve them.


Clamps for wood work?

KlarnEvil9 said:
Kloakk said:
KlarnEvil9 said:
That's not the same post, but it's a very similar idea. Although I think the one I remember was poking the needle across the stipe, which leaves the outer surface as a vector for contam.

I think Thurmann is right about collecting the biopsy from the inner tissue, far less possible contams.


Ah I see, can't remember seeing that thread in my search. And if the stipe wasn't this tiny little thing and I had better tools I would take a different and better approach, like Thurman and Headass is saying. I did try to get inner tissue, but I had to use my scalpel to split it and to get a sample, probably got some of the outer tissue to. We'll see in a couple of days I guess. :biggrin:

Speaking of cloning small fruits, how are the pan growers doing it? I am dreaming of getting a hold of a TTBVI print, and after reading baba's pan monotub thread he is saying the right genetics is key, but I can't remember anything about how he is cloning :strokebeard:


Your sample plates looked good, you managed to get a nice, tiny specimen. Well done!

As far as cloning small fruits goes, Josex poke all the way! I think they recommend using a 21ga needle to get the smallest sample possible. But I know pan growers have used this since it's so precise and designed for tiny shrooms.


:takingnotes:I'll try searching again and read the whole Josex poke thread, also, thanks for giving me hope for my first clones, appreciate it.
:seriousthankyou:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9]
    #29097945 -

KlarnEvil9 said:
QQ: What should I put on the table that has my SAB on it to prevent slipping? It's a folding plastic table, pretty slick on top without a towel or something.

I know that a bath towel is probably not the way, but I'm not sure what I should be looking for. A different type of towel? Or some adhesive rubber gasketing around the lip that makes contact with the table?

Current SAB pic:


She's a sight better than the soft sided one I had (I know I suck), but I don't know if the towel is causing problems or helping solve them.


Personally I wouldn’t use the towel or the silicone mat. That mat is defeating the purpose of the wire rack. Anything that settles is landing on the mat right next to your plates instead of below them. Can’t speak to wether or not the towel is causing issues, but I would think thats adding more particulates to get stirred up inside your SAB.

If you still have the lid to your tub you could put that on and then have the towel under the lid.

Edited by Henry Swanson (01/22/25 05:24 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson]
    #29098001 -

Henry Swanson said:
KlarnEvil9 said:
QQ: What should I put on the table that has my SAB on it to prevent slipping? It's a folding plastic table, pretty slick on top without a towel or something.

I know that a bath towel is probably not the way, but I'm not sure what I should be looking for. A different type of towel? Or some adhesive rubber gasketing around the lip that makes contact with the table?

Current SAB pic:


She's a sight better than the soft sided one I had (I know I suck), but I don't know if the towel is causing problems or helping solve them.


Personally I wouldn’t use the towel or the silicone mat. That mat is defeating the purpose of the wire rack. Anything that settles is landing on the mat right next to your plates instead of below them. Can’t speak to wether or not the towel is causing issues, but I would think thats adding more particulates to get stirred up inside your SAB.

If you still have the lid to your tub you could put that on and then have the towel under the lid.


I sprayed the shit out of the towel with my soap water to hopefully lock down anything on the towel. I really can't say if it did its job but someone here recommended it when I first started, so I felt safe repeating it anyway. 🤷‍♂️


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 3
    #29098025 -

Have to give my 2¢ on the glove box situation.

Avoid anything extra, I know others like to add racks or mats, keep it simple. I've ran this basic sab for over ten years with ZERO issues. No upside down tub, no racks, just a tub with arm holes🍺 The plastic containers just plug the arm holes when not in use. I apologize if I stalled the conversation🙏


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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson] * 1
    #29098028 -

Henry Swanson said:
KlarnEvil9 said:
QQ: What should I put on the table that has my SAB on it to prevent slipping? It's a folding plastic table, pretty slick on top without a towel or something.

I know that a bath towel is probably not the way, but I'm not sure what I should be looking for. A different type of towel? Or some adhesive rubber gasketing around the lip that makes contact with the table?

Current SAB pic:


She's a sight better than the soft sided one I had (I know I suck), but I don't know if the towel is causing problems or helping solve them.


Personally I wouldn’t use the towel or the silicone mat. That mat is defeating the purpose of the wire rack. Anything that settles is landing on the mat right next to your plates instead of below them. Can’t speak to wether or not the towel is causing issues, but I would think thats adding more particulates to get stirred up inside your SAB.

If you still have the lid to your tub you could put that on and then have the towel under the lid.


I totally see what you mean, and I agree in general. The lid for this tub is not too flat, it might need some modification love.  But.... the silicone mat became a necessity after multiple rounds of plates sliding around, off the racks, slipped out of my hand a number of times (quickly) and knocked other things over. If I need the wire rack, then I need a surface that will help combat my advanced clumsiness.

(backstory, NOT sob story) - I've really been struggling with dexterity for the last 4.5 years. I was hospitalized after a weird tingling in my feet grew upward to mid-abdomen. Halfway through that process, I had lost the ability to walk. Soon after, I lost use of my hands also. I couldn't hold a glass to drink from or a spoon to feed myself. 3 weeks inpatient, then months of PT/OT to just be able to walk hobble around again (with a cane or walker). But finger dexterity and those fine motor skills were the last to start getting better, I still don't feel like I'm actually still able to play guitar or anything else well. And I know that this intermittent nerve transmission in my hands has limited my cult success because having your hands shake during inoculation or transfers is a pretty sure way to do it wrong.

I am not making excuses for my shitty cult work!

I'm just trying to find the right combination of tools, materials, and teks that I can actually pull off successfully. Right now, the SAB setup seems to be the problem, but what do I know?


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29098055 -

ThurmanMurman said:
Henry Swanson said:
KlarnEvil9 said:
QQ: What should I put on the table that has my SAB on it to prevent slipping? It's a folding plastic table, pretty slick on top without a towel or something.

I know that a bath towel is probably not the way, but I'm not sure what I should be looking for. A different type of towel? Or some adhesive rubber gasketing around the lip that makes contact with the table?

Current SAB pic:


She's a sight better than the soft sided one I had (I know I suck), but I don't know if the towel is causing problems or helping solve them.


Personally I wouldn’t use the towel or the silicone mat. That mat is defeating the purpose of the wire rack. Anything that settles is landing on the mat right next to your plates instead of below them. Can’t speak to wether or not the towel is causing issues, but I would think thats adding more particulates to get stirred up inside your SAB.

If you still have the lid to your tub you could put that on and then have the towel under the lid.


I sprayed the shit out of the towel with my soap water to hopefully lock down anything on the towel. I really can't say if it did its job but someone here recommended it when I first started, so I felt safe repeating it anyway. 🤷‍♂️


I believe that  would work, same concept as they do in car body shop paint booths to keep dust down. I’ve also  been berated by people on discord who use those flexible side “SAB” for telling them those are turbulent air boxes anytime you bump the sides, and they go on to explain how they hose the inside of the whole thing down with soapy water and have had 0 contams. Still doesn’t make those things SAB’s :lol:

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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson] * 1
    #29098116 -

Yeah...probably better than nothin, but ya know...


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29098132 -

The soft-sided ones are de debbul! I had one for quite a while, very mixed success. While I could point a few particular failures that were caused by other issues, I feel like 70-80% of my contams were caused by the Soft Air Billows. I got lucky a couple of times and slipped a fruit or 3 past the contams while they weren't looking.

I'd say it's at least as bad as nothing, but it could be slightly better than nothing at times, as well as slightly worse. Standard deviations and all that. Or the over/under.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29098137 -

My SAB is all busted at one corner I guess from dropping it. I don’t remember dropping it but I must have. It’s getting harder to see in there. Might have to get a new one.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 4
    #29098139 -

i know this
isnt the confession thread but i confess i miss my sab.  i confess my contam rate was lower before i got this stupid ffu


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9]
    #29098143 -

KlarnEvil9 said:
The soft-sided ones are de debbul! I had one for quite a while, very mixed success. While I could point a few particular failures that were caused by other issues, I feel like 70-80% of my contams were caused by the Soft Air Billows. I got lucky a couple of times and slipped a fruit or 3 past the contams while they weren't looking.

I'd say it's at least as bad as nothing, but it could be slightly better than nothing at times, as well as slightly worse. Standard deviations and all that. Or the over/under.


I dont like bustin balls, and if people wanna chasing their tail, then I guess that's up to them. That's all I meant by it. Im sure they suck though, considering the immediate attempts to steer people away from them.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29098152 -

oh im sorry didnt realizw we were talkin bout soft sabs. nah those are just mold machines.


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #29098165 -

Hey everyone, nice to meet you all! I'm new to this awesome forum with, like, super awesome members.

Since I'm a newbie here, please let me know if I'm posting in the wrong thread or anything.

So, I've been into mm for 13 years now, and still am. In my country (it's in Southeast Asia with a huge Muslim population, you probably know which one 😉),  mm is, uh, pretty illegal. So I've always had to find my own or buy from specific places.

Recently, I tried growing mm using spore prints and spore syringes. I managed to collect some spores (see the pic, sorry it's kinda blurry).  Now, I have some questions:

1. I'm waiting for my substrate to be ready, should be a few days, max 7. What should I do with the spores I have now? I just folded the aluminum foil and kept it in a sterile place. Is that okay?  I have my reasons for waiting on the substrate, it's a long story.

2. If I'm storing the spores right, can I just mix them with distilled water and inject them into the substrate when it's ready?

3. Assuming number 2 is right, and the mycelium forms, I learned that you're supposed to mix the mycelium with the substrate. Then, you get a container lined with black plastic, put a layer of substrate down, then the mycelium mix on top of that, and then another layer of substrate. Is that how it works? Any tips on how thick each layer should be?

4. What's the best way to store the container? Like, temperature, light, and do I need to water it?

5. How long does each stage take until harvest?

6. Can I harvest more than once? How do I take care of the substrate after harvesting?

Wow, that's a lot of questions, haha.  Huge thanks in advance to any experts who can help me out!



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Re: Ask Quick Questions, Get Quick Answers [Re: spirituallyaware]
    #29098167 -

You can just keep them folded in a bag in a fridge with some desiccant. Should be fine.

Welcome to the site.


--------------------

The Shroomery Manual
Wanna Play?
Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #29098370 -

BeefSupremeJr said:
i know this
isnt the confession thread but i confess i miss my sab.  i confess my contam rate was lower before i got this stupid ffu


Assuming the FFU is good, I wonder if your muscle memory is screwing you now - maybe you're very careful to avoid hovering over plates/jars/whatever (which isn't nearly as dangerous in horizontal flow), but you're not noticing when you move non-sterile stuff between the filter and the plates etc.

I found that a bit tricky to remember at first. :shrug: Hope you figure out the problem soon!


--------------------
LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29098374 -

Phaethon said:
BeefSupremeJr said:
i know this
isnt the confession thread but i confess i miss my sab.  i confess my contam rate was lower before i got this stupid ffu


Assuming the FFU is good, I wonder if your muscle memory is screwing you now - maybe you're very careful to avoid hovering over plates/jars/whatever (which isn't nearly as dangerous in horizontal flow), but you're not noticing when you move non-sterile stuff between the filter and the plates etc.

I found that a bit tricky to remember at first. :shrug: Hope you figure out the problem soon!


I absolutely gorilla fucked a ton of work like this recently. I've since learned that I need to walk away when I get frustrated bc my brain straight up leaves the building.

Just showing up to +1 to first air being a really big deal. 🤙


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29098390 -

ThurmanMurman said:
Phaethon said:
BeefSupremeJr said:
i know this
isnt the confession thread but i confess i miss my sab.  i confess my contam rate was lower before i got this stupid ffu


Assuming the FFU is good, I wonder if your muscle memory is screwing you now - maybe you're very careful to avoid hovering over plates/jars/whatever (which isn't nearly as dangerous in horizontal flow), but you're not noticing when you move non-sterile stuff between the filter and the plates etc.

I found that a bit tricky to remember at first. :shrug: Hope you figure out the problem soon!


I absolutely gorilla fucked a ton of work like this recently. I've since learned that I need to walk away when I get frustrated bc my brain straight up leaves the building.

Just showing up to +1 to first air being a really big deal. 🤙


The most important thing I've learned about the SAB or other "high pressure" work is to first practice. Simulate a transfer session where mistakes DO NOT MATTER. Set up some simulated plates and whatever and just go in there and practice start to finish doing your process.

I do a process where I extract clone tissue from the inside of a mushroom with a syringe. Every time I did it I'd get super stressed. So what I did from one grow is I took 10 mushrooms as practice and injected the syringe, extracted the clone tissue, squirted into a simulated liquid culture jar and by like the 7th try, my movements were smooth, quick and confident.  And I just tossed the 10 practice mushrooms (BFD as I have way more than I need) and the next time I had to do it, I was relaxed and completed it way faster with much less wasted movements. I found that if the only time I do these procedures is "under pressure to do it right", then it's way more stressful. Practice in a low pressure situation where the outcome doesn't matter and your stress will go down and your confidence and skill will go up.


--------------------
“Order is needed by the ignorant but it takes a genius to master chaos.”
― Einstein


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Re: Ask Quick Questions, Get Quick Answers [Re: KauaiOrca]
    #29098397 -

Absolutely. I did a ton of agar work in the beginning like this. It was live fire, but I was doing way more transfers than I needed to do, and that was the mental safety net for me. Didn't matter if one shit the bed bc I did 5 others just for shits and gigs.

Edit: since I've learned to identify hidden contams a bit, it was probably a higher fail rate, but the concept remains. I just needed to call myself out. 🤣


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

Edited by ThurmanMurman (01/22/25 11:53 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 2
    #29098456 -

Quote:
just for shits and gigs.

  =  Shiggles

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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