Quote:

LewDoja said:
So when you grab a mushroom to clone for whatever reason ( speed, size, potency) it's good to also take prints from that mushroom.

Then run the clone to see if the desired Traits are expressed,  then germinate the spores collected from the donor. Grow out MS.

And look for desirable traits. Print & clone.
Run clone to see if what was desired is genetic.

Once you find what you're looking for being consistent.

Keep taking prints but keep the culture stored and use a master to keep expanding it indefinitely.

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On a scale of 1-10 how necessary/beneficial is it do this sort of selection on common varieties like b+, gt, ape? I plan on doing this but it's not like I'm starting from scratch, I guess is my point, right?

Also for indefinite storage what is your preference? I've been planning on doing Easy LI for Preservation and Propogation.

I've also got some slants made and some local turkey tail stored for when I decide to try to grow it out of a bucket .

Huskies said:

So I actually never grew nor took PEs before. I made some fresh tea of APE, 40ish grams wet.

Was going to split it with the gf, effectively 2gs each. BUT, I don't know how potent these ones are.

Should I start with just 1g each in case its a 3x in potency? I'm down for 4gs tonight, but not necessarily 6g if ya understand what I mean.

What's your ape dose?

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ThurmanMurman said:

DV8DUG said:

ThurmanMurman said:

Ok, so this tub was a clone of a mutant whose cap grew inside out. Im not seeing that expressing here, but I like those two tall bois in the back left. At this point, I would just be cloning a clone and instead, need to take a print from one of these, yes?

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I am in the same lane as you as we drive down clone Ave. My clone is currently colonizing grain but from my research I have learned that what we should do when our clones grow and don't show the pheno we want, we then print and fruit again until it resurfaces. I could be wrong but this is the road I'm on.

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I understand that grabbing a clone and fruiting it is a way to verify the mutation is genetic, which in my situation, doesn't seem to be the case.

Now as far as the selective breeding side of it, I'm still foggy on what it is we're doing when grabbing a clone. I thought that fruiting a clone was essentially just running the same culture again, but if that's the case, then it wouldn't matter which fruit you cloned. Or maybe I missed a step in the process there and cloning a fruit grown from a clone is when it's just repeating the culture?

I guess I dont understand what to expect from a clone. If I fruit an MS tub and grab a clone, will I then see the clone tub lean toward the characteristics of the clone, or am I just repeating the MS culture to ensure I see another big fruit so that I know it's genetic and then germinate spores from that fruit to get me to the point of having an increased number of big fruits?

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BeefSupremeJr said:

killedSanta said:

21 days after grain to spawn with SFF shoebox tek.
Is it normal to have little puddles like that?
I kept it close and latched the whole time. Should I supply it with FAE? because the moister seems really high

---

This will not be a quick answer, pard. bear with me.

So this is a good example of what zero fae does to a sub.  This should have fruited a long time ago.  At this point, water has matted the myc completely flat and there is very little possibility for this surface to pin.  It seems like there were a lot of things leading up to this that were correct, then at the fruiting state we kinda missed some critical information.

I'd read Comebackkid's writeup on surface conditions first. That ship has kindof sailed.
Theres unmodified tubs  but this involves a loose lid that allows for air exchange.  Yours appears to be one with a gasket and was snapped down snug. We call this 'choking'.  This thing choked.

Can we salvage it? Perhaps. I know others will tell you to dump it and start over but I tend to think you should always try to salvage what you can.
We'll need a brand new surface first.  What would I do? We'll being that you don't have anything else to fall back on, and you can't break whats broke, Id blast that surface off with the dish sprayer if you can.  It looks pretty solidified. If you have a removable shower head, you might have to try that out? I just went through this with another guy in another thread you might check it out.  Im not sure how he got his matted surface off I think he used kitchen utensils or something.

next, he kind of rolled his cake in some field capacity coir and set it in a watertub of his own making.  Totally unconventional but it actually looks like he'll get a new surface and might actually pull some shrooms. I dont think you'd need a water tub really, just a fresh layer of damp coir on top should give you enough moisture.

IN THE MEANWHILE, and what both of you should do, is start from the beginning of research to make sure you're not missing other critical elements. Pay CLOSE ATTENTION to comebackkids write-up on surface conditions as that will determine whether or not you are able to salvage anything from this project. Read the Hitchhikers guide linked in my signature from beginning to end and if you're unclear on anything read it again and then ask questions.  Choose your path if you want to continue this hobby and I'm happy to help you get wherever you want to get.

Good luck pardner.


At a second look, it kindof looks like there might be some pins forming.  Don't be shy.  Take the lid off and show us a good high quality picture of that surface please.  You might be able to get it to fruit and bypass that whole torture and punishment thing i said earlier. I can usually tell by these kinds of pictures that you were worried to open your tub for fear of contamination  which would indicate that you're getting information from reddit? I could be wrong.

If you look at my photo gallery, you'll see that I have no fear of opening lids. Youll see i have a bunch of holes in my tubs with no filter at all allowing normal airflow in and out of the tub at all times from the moment i spawn until the day I harvest and dump the sub. You see all that condensation on your lid? Yeah thats basically just raining back down on your substrate and matting.  You always want to take the lid off and shake those big droplets off once in a while if you're getting them.  Ideally, you don't get much rain until well into the second harvest in my experience, (if your fae is correct)

---

Thank you very much for the details.
I was trying to strictly follow the tek which says that you should only open the tub 24 hours before fruiting.
Also yes, I was reading non-sense on reddit that might got to me.

Anyway, I drained the puddles on the surface and opened it to get a better shot.

I flipped the lid so FAE will go through the cracks. Is this enough? Anything else you think I should do?

phytophthora said:

I feel like there are too many variables at play here to answer that with any kind of usefulness.

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KauaiOrca said:

If you were to put a drop of very clean spores in the center of an agar dish, how many days (typically, on average) would you expect before you would see about a 2 inch in diameter growth circle from it? (Let's assume there were no visible contams)

And then, hypothetically assuming it grew out healthy, how many days before a plin would probably show up?

---

2 to 4 weeks

2 more weeks

variables: genetics, temperature, nutrition medium, size of the plate

random existential fact: we live on average 4000 weeks, that's a limited amount of plins

killedSanta said:

BeefSupremeJr said:

killedSanta said:

21 days after grain to spawn with SFF shoebox tek.
Is it normal to have little puddles like that?
I kept it close and latched the whole time. Should I supply it with FAE? because the moister seems really high

---

This will not be a quick answer, pard. bear with me.

So this is a good example of what zero fae does to a sub.  This should have fruited a long time ago.  At this point, water has matted the myc completely flat and there is very little possibility for this surface to pin.  It seems like there were a lot of things leading up to this that were correct, then at the fruiting state we kinda missed some critical information.

I'd read Comebackkid's writeup on surface conditions first. That ship has kindof sailed.
Theres unmodified tubs  but this involves a loose lid that allows for air exchange.  Yours appears to be one with a gasket and was snapped down snug. We call this 'choking'.  This thing choked.

Can we salvage it? Perhaps. I know others will tell you to dump it and start over but I tend to think you should always try to salvage what you can.
We'll need a brand new surface first.  What would I do? We'll being that you don't have anything else to fall back on, and you can't break whats broke, Id blast that surface off with the dish sprayer if you can.  It looks pretty solidified. If you have a removable shower head, you might have to try that out? I just went through this with another guy in another thread you might check it out.  Im not sure how he got his matted surface off I think he used kitchen utensils or something.

next, he kind of rolled his cake in some field capacity coir and set it in a watertub of his own making.  Totally unconventional but it actually looks like he'll get a new surface and might actually pull some shrooms. I dont think you'd need a water tub really, just a fresh layer of damp coir on top should give you enough moisture.

IN THE MEANWHILE, and what both of you should do, is start from the beginning of research to make sure you're not missing other critical elements. Pay CLOSE ATTENTION to comebackkids write-up on surface conditions as that will determine whether or not you are able to salvage anything from this project. Read the Hitchhikers guide linked in my signature from beginning to end and if you're unclear on anything read it again and then ask questions.  Choose your path if you want to continue this hobby and I'm happy to help you get wherever you want to get.

Good luck pardner.


At a second look, it kindof looks like there might be some pins forming.  Don't be shy.  Take the lid off and show us a good high quality picture of that surface please.  You might be able to get it to fruit and bypass that whole torture and punishment thing i said earlier. I can usually tell by these kinds of pictures that you were worried to open your tub for fear of contamination  which would indicate that you're getting information from reddit? I could be wrong.

If you look at my photo gallery, you'll see that I have no fear of opening lids. Youll see i have a bunch of holes in my tubs with no filter at all allowing normal airflow in and out of the tub at all times from the moment i spawn until the day I harvest and dump the sub. You see all that condensation on your lid? Yeah thats basically just raining back down on your substrate and matting.  You always want to take the lid off and shake those big droplets off once in a while if you're getting them.  Ideally, you don't get much rain until well into the second harvest in my experience, (if your fae is correct)

---

Thank you very much for the details.
I was trying to strictly follow the tek which says that you should only open the tub 24 hours before fruiting.
Also yes, I was reading non-sense on reddit that might got to me.

Anyway, I drained the puddles on the surface and opened it to get a better shot.

I flipped the lid so FAE will go through the cracks. Is this enough? Anything else you think I should do?

---

999999999 said:

KauaiOrca said:

If you were to put a drop of very clean spores in the center of an agar dish, how many days (typically, on average) would you expect before you would see about a 2 inch in diameter growth circle from it? (Let's assume there were no visible contams)

And then, hypothetically assuming it grew out healthy, how many days before a plin would probably show up?

---

2 to 4 weeks

2 more weeks

variables: genetics, temperature, nutrition medium, size of the plate

random existential fact: we live on average 4000 weeks, that's a limited amount of plins

---

BeefSupremeJr said:

Oh good Im' glad you saw my reply.

Yeah these are better pictures and it looks like we have some pins forming so this is good. I'm not sure what tek you're referring to following, but yes, I think you may want to get some fae going.  I always have the 'Ez Dialed' hole pattern in my tubs of any size, that is,

Five holes on each end, towards the top, and three down each side towards the middle of the tub.  You can just melt them in with screw driver heated with a torch. I do it with an exhaust fan so I"m not breathing nasty fumes.  I think you'll get some fruit soonish if you can get adequate fae.  I always struggled with lid-flipping. I prefer holes. Its a lot more set and forget.

Anything else you should do: Read HHG (at least part one) Read Comebackkids surface condition thread.  Stay away from reddit, stay away from youtube.  Whatever Tek you're following sounds like its probaly also obsolete or just based on bad info. 

Keep us informed.  we love seeing people make recoveries.

---

I'll make holes for the next run. At the moment, I'll try to flip the lid for some FAE.
So as I understand from you- I shouldn't be worries that the FAE isn't going through some HEPA filter, right?

I'll definitely do more reading on the Shroomery. And I really hope this one will recover, because I am about to give up growing after 6 months of failures

Also, should I mist the lid lightly? Or does it already have enough moister as is?


Thank you for taking the time to answer me

Janus62 said:

Hi all.

Despite having success with Pans and lions mane fruited in tubs (the latter using a computer fan for FAE and a fogger for humidity), I'm looking to set up my first martha.

It'll be in a spare room, but I don't want the noise of a bathroom extractor fan.  In my tubs, a computer fan is plenty, but I doubt it'll be sufficient for a tent despite my looking at a very small one about 50cm x 60cm x 125cm.  (If that's too small for a couple of trays and 2 LM bags - please correct me!)

Any suggestions for something 'in between' that will provide FAE without a lot of noise would be much appreciated. 

Thanks :-)

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killedSanta said:

BeefSupremeJr said:

Oh good Im' glad you saw my reply.

Yeah these are better pictures and it looks like we have some pins forming so this is good. I'm not sure what tek you're referring to following, but yes, I think you may want to get some fae going.  I always have the 'Ez Dialed' hole pattern in my tubs of any size, that is,

Five holes on each end, towards the top, and three down each side towards the middle of the tub.  You can just melt them in with screw driver heated with a torch. I do it with an exhaust fan so I"m not breathing nasty fumes.  I think you'll get some fruit soonish if you can get adequate fae.  I always struggled with lid-flipping. I prefer holes. Its a lot more set and forget.

Anything else you should do: Read HHG (at least part one) Read Comebackkids surface condition thread.  Stay away from reddit, stay away from youtube.  Whatever Tek you're following sounds like its probaly also obsolete or just based on bad info. 

Keep us informed.  we love seeing people make recoveries.

---

I'll make holes for the next run. At the moment, I'll try to flip the lid for some FAE.
So as I understand from you- I shouldn't be worries that the FAE isn't going through some HEPA filter, right?

I'll definitely do more reading on the Shroomery. And I really hope this one will recover, because I am about to give up growing after 6 months of failures

Also, should I mist the lid lightly? Or does it already have enough moister as is?


Thank you for taking the time to answer me

---

Janus62 said:

... I'm looking to set up my first martha.,,
Any suggestions for something 'in between' that will provide FAE without a lot of noise would be much appreciated. 
Thanks :-)

---

Almost no one does Martha tents here anymore. Most of the poeple growing fat canopies of shrooms are just doing tubs or shoeboxes.

Make it up to field capacity, they're largely set and forget. May need misting occasionally if you're somewhere dry or running a heater fan in a closet on a humidifier in the winter.

But you don't need fans, pumps, or humidifiers for this hobby. That shit is old tek that's been surpassed by simpler quiet things.

Curious, where did you get the idea that it's needed? Something old?

Read my sig line "from spores to trips."