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Re: Ask Quick Questions, Get Quick Answers [Re: bluespirit13] * 6
    #29093840 -

No water involved.

This is how I do transfers.
Less is more.


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“All this buttoning and unbuttoning”

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Re: Ask Quick Questions, Get Quick Answers [Re: 999999999] * 1
    #29093864 -

Pretty slick. 
999999999 said:
No water involved.

This is how I do transfers.
Less is more.


Pretty slick, I like it. Never occurred to me to sample a cake with the lid on, but that would do it Allright.  I've been off the cakes decades for a reason tho, to the agar with it!


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Currently hovering between lurking and post&ghost.  Time to lurk and get to work

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Re: Ask Quick Questions, Get Quick Answers [Re: 999999999] * 1
    #29093881 -

999999999 said:
No water involved.

This is how I do transfers.
Less is more.


You're just stabbing the receiving plate and leaving behind enough cells to initiate takeoff? Thats slick as hell man. I might need to try something like this myself.


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Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: 999999999]
    #29093904 -

What size nail is that lol I wanna try


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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29093917 -

ThurmanMurman said:


Ok, so this tub was a clone of a mutant whose cap grew inside out. Im not seeing that expressing here, but I like those two tall bois in the back left. At this point, I would just be cloning a clone and instead, need to take a print from one of these, yes?


I am in the same lane as you as we drive down clone Ave. My clone is currently colonizing grain but from my research I have learned that what we should do when our clones grow and don't show the pheno we want, we then print and fruit again until it resurfaces. I could be wrong but this is the road I'm on.


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
🅃🄴🄰🄼 ​ 🄲🄾🄽🄳🄸🄼🄴🄽🅃 ​ 🄲🅄🄿

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29093920 -

For cloning, for puks, for agar... tho, on agar, I scrap/scoop, with the needle, more myc to speed up the process; tho, even if you just poke agar and don't see anything in the receiving plate, the myc cells are there - you've just contaminated the agar with myc :muppet: - and will grow concentrically.

whiski: no no, don't poke cakes with the lid on. I was referring to transferring a sample of my from brf puks to agar in a SAB/hood directly, without the jar lids. Everytime a needle touches/frictions a medium is another avoidable vector of contamination.


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“All this buttoning and unbuttoning”

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Re: Ask Quick Questions, Get Quick Answers [Re: 999999999]
    #29093943 -

Quote:
whiski: no no, don't poke cakes with the lid on. I was referring to transferring a sample of my from brf puks to agar in a SAB/hood directly, without the jar lids. Everytime a needle touches/frictions a medium is another avoidable vector of contamination.


But I'm scared to open it! Seriously though, when I was grinding up brf cakes with a Mason jar on a blender... I would have probably flamed needle, halfway in, let cool, stab, stab into agar in ppe 5 containers or whatever it was. Agar grinds much easier than cakes!(also tastes better)


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Currently hovering between lurking and post&ghost.  Time to lurk and get to work

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Re: Ask Quick Questions, Get Quick Answers [Re: whiski]
    #29093982 -

Is it safe to assume if all tubs were started from the same LC then all fruits from said tubs will have the same potency?

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29094014 -

DV8DUG said:
ThurmanMurman said:


Ok, so this tub was a clone of a mutant whose cap grew inside out. Im not seeing that expressing here, but I like those two tall bois in the back left. At this point, I would just be cloning a clone and instead, need to take a print from one of these, yes?


I am in the same lane as you as we drive down clone Ave. My clone is currently colonizing grain but from my research I have learned that what we should do when our clones grow and don't show the pheno we want, we then print and fruit again until it resurfaces. I could be wrong but this is the road I'm on.


I understand that grabbing a clone and fruiting it is a way to verify the mutation is genetic, which in my situation, doesn't seem to be the case.

Now as far as the selective breeding side of it, I'm still foggy on what it is we're doing when grabbing a clone. I thought that fruiting a clone was essentially just running the same culture again, but if that's the case, then it wouldn't matter which fruit you cloned. Or maybe I missed a step in the process there and cloning a fruit grown from a clone is when it's just repeating the culture?

I guess I dont understand what to expect from a clone. If I fruit an MS tub and grab a clone, will I then see the clone tub lean toward the characteristics of the clone, or am I just repeating the MS culture to ensure I see another big fruit so that I know it's genetic and then germinate spores from that fruit to get me to the point of having an increased number of big fruits?


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: Jebi] * 1
    #29094016 -

Jebi said:
I do 2 hours in IP for PF jars.



Really?  2-hour seems like major overkill for pf.  Isn't the recommended time 90 minutes?

I mean that's recommened for a PC and IC may be little bit different i guess Or is it?


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:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 1
    #29094021 -

callmehippie said:
Jebi said:
I do 2 hours in IP for PF jars.



Really?  2-hour seems like major overkill for pf.  Isn't the recommended time 90 minutes?

I mean that's recommened for a PC and IC may be little bit different i guess Or is it?


IP isn't getting to 15psi which means it doesn't get as hot, which means it's going to take longer to reach temp throughout the sub.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29094033 -

BeefSupremeJr said:
Jrey16 said:
Not my picture but wondering what causes the caps to get like that? Is it mycelium? And what's up with the very fluffy mycelium on the substrate? This is Tidal Wave





iono but those pics in your signature are nice as fuck


Wow thanks haha. I wanna experience growing some bigger fruits though like those 2 pics I posted. The reason why I'm asking why those fruits and mycelium look like that is because someone is hooking me up with those same exact genetics. I was wondering if they look like that due to the genetics or is it because of how the person grew it.


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Re: Ask Quick Questions, Get Quick Answers [Re: bchino]
    #29094068 -

bchino said:
Is it safe to assume if all tubs were started from the same LC then all fruits from said tubs will have the same potency?


I don’t think that assumption is 100% safe. First of all I think it depends what was on the plate that you started the LC from.
If it is a multispore culture you likely still have multiple strains there, maybe if you then start the LC with the “Josex poke” you get less variation?
If you had a clone there then I guess you have even less variation, but even then my understanding is that even the mushroom body is made up from multiple strains. Not sure how much the potency of these strains can differ though. I guess with a clone this assumption is pretty safe, from a practical standpoint at least.
Now if you’d have a actual monoculture then this assumption can be actually absolutely true.

Also I’m guessing that there might be some environmental factors playing a role. After all even if You have two clones of anything (think twins separated at birth maybe?), nurture still plays some role.

Someone should confirm my line of thought. Maybe in the real world, this all amounts to negligible differences.

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29094071 -

I would say 2 will generally equal 4 with PE varieties. Wouldn’t push it first time if you don’t wanna accidentally go 6 like you said haha

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Re: Ask Quick Questions, Get Quick Answers [Re: bchino]
    #29094081 -

bchino said:
Is it safe to assume if all tubs were started from the same LC then all fruits from said tubs will have the same potency?


Only if you made the LC from a plate with clone culture. It's as simple as that.


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--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: eilauver] * 2
    #29094082 -

eilauver said:
bchino said:
Is it safe to assume if all tubs were started from the same LC then all fruits from said tubs will have the same potency?


Now if you’d have a actual monoculture then this assumption can be actually absolutely true.


You said the right answer, "clone", then kept going with misinformation.

Monoculture isn't a thing. It's old, wrong info. I'd recommend not giving advice when you're this new with things you don't know about.

Most of us did this early on, and most of us got told not to. It's the difference between this place and Reddit shroom subs. (where wrong answers often get "karma" points.)

That's the second time in 10 minutes here that I've debunked "monoculture." Curious where you heard that? Stamets?


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61] * 3
    #29094134 -

Quote:
That's the second time in 10 minutes here that I've debunked "monoculture." Curious where you heard that? Stamets?


Probably pieced it together from bits and pieces from here, that could easily have been outdated or misleading.

I in no way was tying to imply that it’s something one can achieve. In my head it just seemed reasonable to think that the least amount genetic diversity you have in your culture, more likely it is that each fruit is with the same potency.

Now it seems that term I used for that is not quite it. Maybe I meant a single dikaryotic strain, who knows. That’s how misinformation moves, people talking about things they actually do not understand. I’ll reel myself in.

Edited by eilauver (01/19/25 03:04 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 3
    #29094135 -

So when you grab a mushroom to clone for whatever reason ( speed, size, potency) it's good to also take prints from that mushroom.

Then run the clone to see if the desired Traits are expressed,  then germinate the spores collected from the donor. Grow out MS.

And look for desirable traits. Print & clone.
Run clone to see if what was desired is genetic.

Once you find what you're looking for being consistent.

Keep taking prints but keep the culture stored and use a master to keep expanding it indefinitely.

Safe guarding prized cultures is the long-game.

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29094173 -

Hi all.

Despite having success with Pans and lions mane fruited in tubs (the latter using a computer fan for FAE and a fogger for humidity), I'm looking to set up my first martha.

It'll be in a spare room, but I don't want the noise of a bathroom extractor fan.  In my tubs, a computer fan is plenty, but I doubt it'll be sufficient for a tent despite my looking at a very small one about 50cm x 60cm x 125cm.  (If that's too small for a couple of trays and 2 LM bags - please correct me!)

Any suggestions for something 'in between' that will provide FAE without a lot of noise would be much appreciated. 

Thanks :-)


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Ask Quick Questions, Get Quick Answers [Re: Janus62]
    #29094182 -

First  off, nowadays most bathroom fans are damn near silent.

But there's many ways to achieve this...

Just recently saw someone use an inline fan connected to a tote. Fan is always running but the fogger inside tote was on timer.

Poked some holes in the top plastic.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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