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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Radamanto]
    #29078087 -

I have no concrete evidence, but I suspect that pointed upwards and With and airgap in the syringe, will help even better.

"When water boils, it expands significantly, roughly 1,600 times its original volume as it turns into steam, meaning one liter of liquid water becomes approximately 1,600 liters of steam."

the airgap helps to additionally account for expansion of liquid water

"Water expands by about 4% when heated from room temperature to its boiling point."

Edited by Jebi (01/05/25 01:28 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Jebi]
    #29078092 -

I forget which trusted cultivator said it, but I know one of them mentioned that they just boil water and suck it up directly. They say that the heat from the water is sterilizes the inside of the syringe. I've never tried it though I just usually make a couple while I'm making agar


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: Churpee Choozle] * 1
    #29078095 -

Churpee Choozle said:
Shit dat wordt voor het eerst in 1000 jaar naar de Dirk


Oh, getting snobby, eh? :wink:

These are them btw:


Seems like I lied. I think I said they were 1.99, but actually they are cheaper, like 50 cents, so 1.49.

If the rain stops, I will do a walk of shame in a few minutes.

I assume they are OK to PC, but will find out soon. The cap is rubbish. Also not clear what kind of plastic.

@Tweeq: I use kind of the same lids as you use. I basically reuse standard lid jars of both plastic and metal.

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Re: Ask Quick Questions, Get Quick Answers [Re: Jebi] * 1
    #29078105 -

I did a test some time ago. And used syringes filled with water to 9ml, and screw cap on them. Quickly after PC cycle the
Jebi said:
I have no concrete evidence, but I suspect that pointed upwards and With and airgap in the syringe, will help even better.

"When water boils, it expands significantly, roughly 1,600 times its original volume as it turns into steam, meaning one liter of liquid water becomes approximately 1,600 liters of steam."

the airgap helps to additionally account for expansion of liquid water

"Water expands by about 4% when heated from room temperature to its boiling point."


I did a test some time ago. I filled syringes with water to 9ml, and screw cap on them. Quickly after PC cycle the volume was increased. After a cool down period, the volume returned to 9ml.

Quickly after PC:


After cool down period:

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Re: Ask Quick Questions, Get Quick Answers [Re: cybork]
    #29078118 -

smacks head.  Caps! Genius.

And a real life example of fluid dynamics too.  It looks like it expanded about 1/3rd?  from 9-12ml.

:rockon:

Edited by Jebi (01/05/25 02:16 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Jebi]
    #29078123 -

I forgot about these BRF jars

Keep or toss?  Nearly 2 months old.  I think it's metabolytes.



pretty much already decided to toss this one, but I got a good up close picture of some kinda blueish/green mold.  (It's stuck to the jar, where the rest of the cake is shrunken.)


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Re: Ask Quick Questions, Get Quick Answers [Re: Jebi]
    #29078345 -

Jebi said:
I forgot about these BRF jars

Keep or toss?  Nearly 2 months old.  I think it's metabolytes.



pretty much already decided to toss this one, but I got a good up close picture of some kinda blueish/green mold.  (It's stuck to the jar, where the rest of the cake is shrunken.)





I would go for that top one. Birthday dunk it maybe put it in a shoe box by itself or something if you're worried. But I just recently had three or four jars that look just like that and they're doing fine right now no signs of contamin or anything. It was just metabolites and they washed right off. Not saying that's the case where you but it's could be.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29078347 -

Just curious I'm a still pretty new to the game so I'm not sure. In regards to all these questions about sterilizing syringes with water. Could you not just put like a glass container in the microwave and boil some water and then draw it up or does that not work? I have a feeling everybody would be doing this if it worked so is this a dumb question? lol.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29078355 -

LewDoja said:
Not sure about ip but 15 mins at 15psi would work. Probably more than needed but why risk it if it's already up to temp


In my notes (copied/pasted from here somewhere), I have 30min in the IP (manual/high pressure) to sterilize water, syringes, or foil wrapped tools.

Remember that you can kinda vent your IP by leaving the valve open. Once steam is coming out in a stream, wait 5-10 min then close it, then the cycle will start pretty quickly.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon]
    #29078386 -

CaptainPuffy said:
I forget which trusted cultivator said it, but I know one of them mentioned that they just boil water and suck it up directly. They say that the heat from the water is sterilizes the inside of the syringe. I've never tried it though I just usually make a couple while I'm making agar


I believe you're thinking of Bod's Spore Syringe Tek.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 1
    #29078394 -

Stipe covers it in his streaking vid too.


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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson] * 1
    #29078446 -

If we are never putting spores to grain, then what is really the point of putting them in a syringe? Just so we can poke them through holes in the top of a PF tek jar?

Is getting good at this a prerequisite for making LC syringes?

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Re: Ask Quick Questions, Get Quick Answers [Re: Lilgrower] * 7
    #29078457 -

Where we're going, we won't need LC.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Ask Quick Questions, Get Quick Answers [Re: Lilgrower] * 3
    #29078472 -

Lilgrower said:
If we are never putting spores to grain, then what is really the point of putting them in a syringe? Just so we can poke them through holes in the top of a PF tek jar?

Is getting good at this a prerequisite for making LC syringes?


I’ve got a few different types of spores that are 3-4 years old that won’t germ in agar that I am force hydrating in syringes. But I just sterilize water in a jar with a modified lid and suck it up a new sterile syringe and needle in front of a flow hood. Then scrape spores from print into a sterilized receiving jar and splash them with the water, suck it back up and cap them. After that, throw a rubber band or two from plunger to tip. We will see how these few I have going do in a day or two.


--------------------

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Re: Ask Quick Questions, Get Quick Answers [Re: Lilgrower]
    #29078473 -

For me specifically, yes I was making a syringe to use for PF tek / BRF pucks.

The PF jars would get fruited if successful, and the pucks would get transferred to agar to clean up most likely.


--------------------


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Re: Ask Quick Questions, Get Quick Answers [Re: bluespirit13] * 1
    #29078551 -

When purchasing WBS I've noticed it says to make sure there's no chemical additives or fungicides.

However I'm noticing in lots of the different options I'm looking at they do have added nutrients such as vitamins and minerals. Is this okay? or am I supposed to be finding some that is completely free of additives of any kind?

Do they make it completely free of everything including vitamins and minerals?

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 1
    #29078553 -

callmehippie said:
When purchasing WBS I've noticed it says to make sure there's no chemical additives or fungicides.

However I'm noticing in lots of the different options I'm looking at they do have added nutrients such as vitamins and minerals. Is this okay? or am I supposed to be finding some that is completely free of additives of any kind?

Do they make it completely free of everything including vitamins and minerals?


disclaimer - Noob here, have <20 grows under my belt but trying to learn.

I've been using a Finch mix which is predominately millet with vitamins added. I give it a good wash with hot water before soaking and haven't had any issues across the 8 tubs I've done. 

Here's the ingredient list Red Millet, Canary Seed, Japanese Millet, Shirohie Millet, Panicum, Panorama Millet, White French Millet, plus Vegetable Oil, Iodine, Vitamin A, Vitamin D3, Vitamin E.

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 2
    #29078554 -

callmehippie said:
When purchasing WBS I've noticed it says to make sure there's no chemical additives or fungicides.

However I'm noticing in lots of the different options I'm looking at they do have added nutrients such as vitamins and minerals. Is this okay? or am I supposed to be finding some that is completely free of additives of any kind?

Do they make it completely free of everything including vitamins and minerals?


I used Pennington’s for a year and a half or so and it has added nutrition. Just give it a good wash with hot water and a tiny bit of dish soap and you’re good. It does t hurt anything


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Lilgrower] * 4
    #29078562 -

Lilgrower said:
If we are never putting spores to grain, then what is really the point of putting them in a syringe? Just so we can poke them through holes in the top of a PF tek jar?

Is getting good at this a prerequisite for making LC syringes?


Spore syringes are caveman tek.

It's a good thing to strive to graduate away from.

One reason spore syringes are still around is people make money off them. You can make about 10 to 50 syringes from a spore print.

Some guy on Reddit told me a few years ago he gets free spore prints here from the Marketplace then makes a bunch of syringes from it and sells them on Reddit.

It takes a very small amount of spores to make a good spore syringe.

Often if you see black things floating in a purchased syringe it's powdered activated charcoal to look like it's dense with spores.

A usable spore syringe can look to the naked eye like water and nothing else.

Spore syringes are to this hobby what training wheels are to a bike. No shame in starting with them, but you don't want to use them forever.

Work toward:
--spores from print or swab to
--agar Petri dish to
--transfer a small amount to another agar Petri dish (to clean it up)
--to PCed grain jar
--to shoebox plastic tub, usually with some coir and vermiculite added. From there the shrooms grow.

This is called bulk tek, as opposed to a PF tek, which it sounds like you're doing.

There are many tutorials on here. Bottom link on my sig line has some good ones.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61] * 1
    #29078577 -

Groovy. Thanks guys


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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