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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator] * 1
    #29065448 -

AspectOfTheCreator said:
Created this uninoculated jar as a control due to mysterious contam issues. How long without contam must I wait before I can deem my grain prep/pc cycle/lid a success?



Lots of starch on glass. Had burst grains from prep. The inoculated jars seem to be colonizing fine.




I asked a similar question once and was told that I likely wouldn't notice bacteria on uncolonized grain.

Im also at a place where I need to test my grain prep/lids and Im thinking about dropping half of an uncolonized agar plate in the jar and leaving it where I can see it, which I hope would allow me to see any contamination growth. For the lids, the best I can come up with is to pour agar in half pints, PC with the lids on and then let them sit and see if anything happens. I'd have to test about 50 modded lids that way, but it would ease my mind anyway.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 2
    #29065495 -

ThurmanMurman said:
AspectOfTheCreator said:
Created this uninoculated jar as a control due to mysterious contam issues. How long without contam must I wait before I can deem my grain prep/pc cycle/lid a success?



Lots of starch on glass. Had burst grains from prep. The inoculated jars seem to be colonizing fine.




I asked a similar question once and was told that I likely wouldn't notice bacteria on uncolonized grain.

Im also at a place where I need to test my grain prep/lids and Im thinking about dropping half of an uncolonized agar plate in the jar and leaving it where I can see it, which I hope would allow me to see any contamination growth. For the lids, the best I can come up with is to pour agar in half pints, PC with the lids on and then let them sit and see if anything happens. I'd have to test about 50 modded lids that way, but it would ease my mind anyway.



If they were made the same way shouldn't need more than a few random (or specifically chosen that are questionable).

With two-piece lids, there is a high probability that contamination comes from the fingers during initial opening (the un-sticking), or when lifting for transfer ( holding too far down, especially with loose fitting gloves that can reach further underneath than we're aware of).

Experiment:
Take gloved finger or two after fully sanitizing and press onto a clean plate lightly.


Edited by LewDoja (12/23/24 07:55 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29065521 -

LewDoja said:
ThurmanMurman said:
AspectOfTheCreator said:
Created this uninoculated jar as a control due to mysterious contam issues. How long without contam must I wait before I can deem my grain prep/pc cycle/lid a success?



Lots of starch on glass. Had burst grains from prep. The inoculated jars seem to be colonizing fine.




I asked a similar question once and was told that I likely wouldn't notice bacteria on uncolonized grain.

Im also at a place where I need to test my grain prep/lids and Im thinking about dropping half of an uncolonized agar plate in the jar and leaving it where I can see it, which I hope would allow me to see any contamination growth. For the lids, the best I can come up with is to pour agar in half pints, PC with the lids on and then let them sit and see if anything happens. I'd have to test about 50 modded lids that way, but it would ease my mind anyway.



If they were made the same way shouldn't need more than a few random (or specifically chosen that are questionable).

With two-piece lids, there is a high probability that contamination comes from the fingers during initial opening (the un-sticking), or when lifting for transfer ( holding too far down, especially with loose fitting gloves that can reach further underneath than we're aware of).

Experiment:
Take gloved finger or two after fully sanitizing and press onto a clean plate lightly.




There's not a single brain cell committed to the idea my hands could ever been totally clean, so I'm with ya there. My thing is that my a2a transfers look great and I've had very little obvious contamination. Even if I add up the times where the mycelium looked weird in a spot and attributed it to a hidden contamination, my success rate would still be pretty high, and a far cry from making nothing but bacterial jars. Im testing my lids next and taking it from there.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29065522 -

some things just don't senesce. take pothos. its main method of reproduction is selfing. it rarely flowers even in nature. it's all about how the organism is built and its dna. cultures senesce at different rates based on numerous factors, tweaking them will likely make little difference how much longer or shorter it takes for your agar to have a nice, peaceful, natural death.


--------------------
----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head]
    #29065525 -

My concern is this has been like this for several weeks (almost 4). Its NOT super dry but I don't know if its wet enough. There is ONE mushroom growing...and that's it. Was thinking of getting some of the dirt manure mix and breaking up this mycelium and mixing it with the soil that is more wet and seeing if it improves? Or I wait longer?

Something seems wrong but there's no mold. Just feels like nothing is happening. Any thoughts on how to stim some growth? Should I turn on the humidifier or the fan? Normally it's covered and there are air holes with filters on them. These are PE.




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Re: Ask Quick Questions, Get Quick Answers [Re: DiogeneseK9]
    #29065527 -

I think it's too dry.  You need to mist it more then you currently are.

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Re: Ask Quick Questions, Get Quick Answers [Re: Jebi]
    #29065528 -

Ok thank you. I'll mist it a lot more I think. Should I just break it up and mix with some substrate and try to improve the field?

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Re: Ask Quick Questions, Get Quick Answers [Re: DiogeneseK9]
    #29065531 -

Is germinating spores on water agar practical?


--------------------
Thank your postal workers when you see them. None of this would be possible without them.

Necessity is the mother of invention

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Re: Ask Quick Questions, Get Quick Answers [Re: DiogeneseK9] * 2
    #29065538 -

I actually had an incident quite early right after I started.
Made a bunch of grain jars through G2G, mixed it all up with coir and started making tubs, mini monos, bottles, you name it. I threw that mixed coir and grain into everything.

Interestingly two of those didn’t even start pinning until the first one had given me it’s 3rd or 4th harvest.

I didn’t know what I did at all back then, so there was probably something I did.

I wouldn’t give up yet if I were you ☺️


--------------------
= Pressure needed to reach 121C/250F, should you decide not to vent =
= Cabin Sequestering FAQ (to roof, or not to roof?) =

"Assumption is the mother of all fuckups"

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Re: Ask Quick Questions, Get Quick Answers [Re: DiogeneseK9]
    #29065598 -

I would simply give it enough water.  Looks like all it's lacking to me.

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29065606 -

LewDoja said:
Some say senescence can be mitigated by changing up carbon/nitrogen sources... probably different trace mineral profiles helps.. :shrug:

They are  complimentary  together... the red millet has higher nitrogen content & amino acids... not to mention their physical properties.


We still on this? I answered you 2 weeks ago why senescence isn't an issue in this hobby,
https://www.shroomery.org/forums/showflat.php/Number/29058913#29058913

You don't have senescence, you have mold. It's got nothing to do with senescence.

You need to go back to basics on your tek.

My first year here I was doing what you're doing. Some great agar plates, some good grows, but some some grain jars showing contam (so I'd throw the contents out) and some contammed boxes, it started in the jars and I didn't notice.

How are doing jar lids / filters / and PCing?
I just wrote up a think on that:
https://www.shroomery.org/forums/showflat.php/Number/29065574


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61]
    #29065618 -

nektar61 said:
We still on this? I answered you 2 weeks ago why senescence isn't an issue in this hobby,
https://www.shroomery.org/forums/showflat.php/Number/29058913#29058913





Nah, we're on this ☺️


--------------------
= Pressure needed to reach 121C/250F, should you decide not to vent =
= Cabin Sequestering FAQ (to roof, or not to roof?) =

"Assumption is the mother of all fuckups"

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Re: Ask Quick Questions, Get Quick Answers [Re: MycCycle]
    #29065624 -

MycCycle said:
nektar61 said:
We still on this? I answered you 2 weeks ago why senescence isn't an issue in this hobby,
https://www.shroomery.org/forums/showflat.php/Number/29058913#29058913





Nah, we're on this ☺️


Your grain choice isn't the issue. It's something else. Maybe that you're doing that b.s. Sequestering tek.

That's on PubMed because it's a very advanced tek for labs that are completely sterile.



--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61] * 2
    #29065665 -

Senescence isn't an issue in this hobby because it's exactly that, a hobby. Nobody around here is going from one jar of spawn to 1000 bags.


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr]
    #29065676 -

cronicr said:
Senescence isn't an issue in this hobby because it's exactly that, a hobby. Nobody around here is going from one jar of spawn to 1000 bags.


It keeps coming up though. haha. Along with "fruiting conditions", and putting gypsum and coffee into grows.

Probably because some TC mentioned it 15 years ago. Or from a Paul Stamets book from around the same time that's still in print with no update.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61] * 3
    #29065682 -

nektar61 said:
cronicr said:
Senescence isn't an issue in this hobby because it's exactly that, a hobby. Nobody around here is going from one jar of spawn to 1000 bags.


It keeps coming up though. haha. Along with "fruiting conditions", and putting gypsum and coffee into grows.

Probably because some TC mentioned it 15 years ago. Or from a Paul Stamets book from around the same time that's still in print with no update.


Which is why I wrote “However, done right you should be able to pull a million jars before it becomes an issue” in the original post.
That had nothing to do with my combo grains question which I directed at Rev.
LewDoja just added the mitigation part as his 2 cents.

“Should” bring the operative word here.


--------------------
= Pressure needed to reach 121C/250F, should you decide not to vent =
= Cabin Sequestering FAQ (to roof, or not to roof?) =

"Assumption is the mother of all fuckups"

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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr] * 1
    #29065719 -

cronicr said:
....Nobody around here is going from one jar of spawn to 1000 bags.


Maybe someone has, haha.

(this is just a fun fact, and more for the new people more than cronicr):

Every once in a while, about once a year, someone's first post here is something like

"I've never grown shrooms but I have a business plan I wrote on an envelope. So, I need to grow 500 kilos of shrooms in the next 3 months. What technique and what strain should I use?"


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: MycCycle] * 2
    #29065721 -

Senescence happens to everything. It is the telomeres at the terminal ends of your chromosomes becoming untethered to Weezer's Sweater Song. It looks just like it too. Like a ball of yarn coming slowly unraveled and disintegrating into enzymes.

You should use your media as soon as feasible and the only issue I've ever had with senescence in my trials is that the agar will often dry out before some cultures die. When cloning a very old culture, or a culture that has already been cloned a lot either in an attempt to preserve or out of laziness, I label it as use first to grow out and fruit, like a fifo system.


--------------------
----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Ask Quick Questions, Get Quick Answers [Re: MycCycle] * 5
    #29065795 -

Funny how one word that wasn't even relevant(directly), sends everyone in a spiral. :shakingfist:

Must be a slow night, & ppl be anxious for the coming holidays :shrug: (family's a PITA, until it's not - and then it's worse.)

But Yeah-

Sorry MycCycle 😞 .



HAPPY HOLIDAYS EVERYONE!!

:mushroom2:love

Edited by LewDoja (12/24/24 03:36 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29065804 -

Reliable vendors for ps. Tampanesis spores in the eu

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Shop: Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Substrate Bags

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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