LightShedder said:

Haha it's interesting you say 10 or 15 years because,  as you can notice from my post history,  that's precisely around the time I left for a long hiatus. And you're exactly right...... But thanks for clearing that up for me. Good observation BTW. 👍

K sectoring is bullshit. Duly noted ✅️ 👌

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orca4 said:

Haha that'd be awesome! A little myco lab cafe. I love your set up there. I unfortunately don't the space nor the cake for a flow hood so I need a modestly sized SAB for home lab work.

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I am proud to say I "suffered for my art" too. When I moved to my new place, first place I haven't shared with room mates in decades, it was August and so fucking hot.

I had enough money to either buy a flowhood or a window unit AC. I bought the flowhood, sweated, and waited 2 weeks for next paycheck to buy AC.

Pic Tax, Z-Strain variety, bruising what looks to me more green than blue. Anyone had this? I've had it with Z, with some early x7x+, and with B+ when I first started growing.

nektar61 said:

LightShedder said:

Haha it's interesting you say 10 or 15 years because,  as you can notice from my post history,  that's precisely around the time I left for a long hiatus. And you're exactly right...... But thanks for clearing that up for me. Good observation BTW. 👍

K sectoring is bullshit. Duly noted ✅️ 👌

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Yes! I almost didn't reply, thinking some TC was going to call me a fool, but I'm positive it's correct best practices now that "sectoring is bullshit."

(ESPECIALLY searching for "an individual sector" to have a "pure strain." The shrooms throw up slightly new generics for no reason at all, often.)

People on Reddit will argue with you on that but they never have photos of what they're claiming. Though I'm not sure what photos would prove it, except maybe microscopy.

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CocaineBuffet said:

Yes. Sectoring means to ME that you are "isolating" (not an isolation) a segment of the healthiest growth(s) from one plate to the next in an effort to create a clean culture of mycelium.

No intention of manipulating the characteristics of the future fruit bodies

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ThurmanMurman said:

CocaineBuffet said:

Yes. Sectoring means to ME that you are "isolating" (not an isolation) a segment of the healthiest growth(s) from one plate to the next in an effort to create a clean culture of mycelium.

No intention of manipulating the characteristics of the future fruit bodies

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Right, bc there's no information available until you fruit it, so grabbing sectors doesn't have anything to do with fruiting performance.

I guess I can ask a question that will clear this up easily - will different sectors of an MS culture perform differently if isolated and eventually fruited? If I take 4 transfers from different sectors of the same MS plate, can I then consider those 4 new plates separate cultures that have been genetically segregated from the MS plate?

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CocaineBuffet said:

Yes. Sectoring means to ME that you are "isolating" (not an isolation) a segment of the healthiest growth(s) from one plate to the next in an effort to create a clean culture of mycelium.

No intention of manipulating the characteristics of the future fruit bodies

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Quote:

So, previously I believed that, if one were to reduce genetic diversity to a single culture, he'd have a "monoculture." Upon fruiting, the monoculture would release spores of identical genetics. I would expect that any and all spore prints taken from the fruiting bodies would contain the genetically identical monoculture.

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CocaineBuffet said:

ThurmanMurman said:

CocaineBuffet said:

Yes. Sectoring means to ME that you are "isolating" (not an isolation) a segment of the healthiest growth(s) from one plate to the next in an effort to create a clean culture of mycelium.

No intention of manipulating the characteristics of the future fruit bodies

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Right, bc there's no information available until you fruit it, so grabbing sectors doesn't have anything to do with fruiting performance.

I guess I can ask a question that will clear this up easily - will different sectors of an MS culture perform differently if isolated and eventually fruited? If I take 4 transfers from different sectors of the same MS plate, can I then consider those 4 new plates separate cultures that have been genetically segregated from the MS plate?

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I wouldn't ever expect them to act different. Since I have gone through some personal strain crossings and I would say that your original culture is going to be more representative of that culture given a natural outlier that say; makes big fruits or clusters

I personally believe that there is no kind of fruit/harvest manipulation on agar that can be done when working with spores even with a microscope. Would be glad to be proven wrong though

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nektar61 said:

CocaineBuffet said:

Yes. Sectoring means to ME that you are "isolating" (not an isolation) a segment of the healthiest growth(s) from one plate to the next in an effort to create a clean culture of mycelium.

No intention of manipulating the characteristics of the future fruit bodies

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I think we're saying the same thing...We attempt to transfer the best looking, cleanest, most robust "sector" (section) of agar to the next plate.

But I'd encourage us not using the word "sectoring" or "sector" with this.

It will make some noobs (especially ones who've read Stamets' old old book with so much wrong shit, like adding coffee, and adding gypsum for bulk grow, not just PF tek, where it's used just as lube to get the thing out of the jar)

There is a bullshit idea sometimes called "sectoring" that used to be popular, 10 to 15 years ago here, and still sometimes on Reddit, of "trying to cut the smallest individual sector to transfer, because that is a MONOCULTURE." Which is bunk (and the T80 PE experiment confirms it.)

If you search "monoculture", or  "monoculture" + "sector", and look at really old posts, you'll find this bunkum.

https://www.shroomery.org/forums/dosearch.php?terms=monoculture+sector

Here are dozens of posts on it.
https://www.shroomery.org/forums/dosearch.php?terms=monoculture

Here's one in particular, from 9 years ago:

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CocaineBuffet said:

I was not aware of this given we point people to more relevant information. Nektar and I are on the same page. I must have made the assumption people are aware of the "last 3~ year rule when it comes to teks".

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CocaineBuffet said:

Having a hard time coming up with a term that defines this clearly since there is apparently and overlap with "sectoring" and "isolating"

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ThurmanMurman said:

Im going to jump in here bc I was either very much under the wrong impression, or I'm not understanding properly.

In the few months I've been doing agar work, I was doing so under the impression that sectors were genetically different, and while one can't judge the fruiting capabilities/qualities from the mycelium, we can at least select for the healthiest and fastest growing mycelium, which would at least be a step in the right direction, compared to mycelium that was slow, thin, etc.

Are you saying that sectors are just a way for us to communicate which part of the plate looks healthy and is not a genetically unique "arm" of the plate itself?

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LightShedder said:

ThurmanMurman said:

Im going to jump in here bc I was either very much under the wrong impression, or I'm not understanding properly.

In the few months I've been doing agar work, I was doing so under the impression that sectors were genetically different, and while one can't judge the fruiting capabilities/qualities from the mycelium, we can at least select for the healthiest and fastest growing mycelium, which would at least be a step in the right direction, compared to mycelium that was slow, thin, etc.

Are you saying that sectors are just a way for us to communicate which part of the plate looks healthy and is not a genetically unique "arm" of the plate itself?

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You're explaining it exactly the way I understand it. 👍 I think we're correct in that there are different genetic expressions represented in different sectors on a plate.

I think what nektar is saying is true as well though in that one can never achieve a "monostrain" after transferring endless sectors and that the mycelium will always sector out.

I think.

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Quote:

Base Parent Identifiers from MS: 
- PE-A1 (First Parent) 
- PE-B1 (Second Parent) 

Generation 1: 
PE-A1 
  * PE-A2-A 
    - PE-A3-A1 
      - PE-A4-A1-1 
      - PE-A4-A1-2 
    - PE-A3-A2 
      - PE-A4-A2-1 
      - PE-A4-A2-2 
  * PE-A2-B 
    - PE-A3-B1 
      - PE-A4-B1-1 (Variation 1 of PE-A4-B1) 
      - PE-A4-B1-2 (Variation 2 of PE-A4-B1) 
    - PE-A3-B2 
      - PE-A4-B2-1 (Variation 1 of PE-A4-B2) 
      - PE-A4-B2-2 (Variation 2 of PE-A4-B2) 

PE-B1 
  * PE-B2-A 
    - PE-B3-A1 
      - PE-B4-A1-1 (Variation 1 of PE-B4-A1) 
      - PE-B4-A1-2 (Variation 2 of PE-B4-A1) 
    - PE-B3-A2 
      - PE-B4-A2-1 (Variation 1 of PE-B4-A2) 
      - PE-B4-A2-2 (Variation 2 of PE-B4-A2) 
  * PE-B2-B 
    - PE-B3-B1 
      - PE-B4-B1-1 (Variation 1 of PE-B4-B1) 
      - PE-B4-B1-2 (Variation 2 of PE-B4-B1) 
    - PE-B3-B2 
      - PE-B4-B2-1 (Variation 1 of PE-B4-B2) 
      - PE-B4-B2-2 (Variation 2 of PE-B4-B2) 
this allows you to easily decipher the Transfer (n) as in the last example is the fourth transfer from the original dish no matter which subsequent iteration(s) that it came from

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