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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Mr Piggy] * 1
    #28960522 -

Mr Piggy said:
Oh word?  All of my subtrop plates are pinning :lol::shrug:





Glad rounds til the end

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: stagger] * 7
    #28960684 -

I dropped a plin in LC. It started fruiting in the LC. It’s on grain now. I hope to see fruit in a few weeks. It certainly acts eager to produce.


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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Imatryin] * 1
    #28960969 -

The only times I got agar pins were during the summer, when temperature were about 23°C.
So I'm speculating higher ambient temperature help a lot with agar pins.


--------------------
:mushroom2::heart: Growing Semperviva/Subtropicalis - My standard procedure, it all comes down to the basics. :heart::mushroom2:



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: mush_monkey] * 1
    #28961368 -

I'd like your advice on asset management. I have three BRF pucks



I made three transfers on the 23rd that are looking promising





Pics are terrible but I can't manage to focus past the horrible condensation. The dark parts are vermiculite but the mic is white, a little fluffy but there's rizomorphic areas in the outer sides.

My question is if I can keep the pucks in the fridge for later or would that kill the mycelium. I think pins are already appearing. Thanks in advance!


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Give away list

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: mush_monkey] * 1
    #28964937 -

I think that 2 of the transfers I made from the BRF puck still look promising





A third one has metabolites (the big drop in the center and a smaller one above are not condensation)



So, what's the move? Let the first two grow for a little longer and inoculate a grain jar or make another transfer?


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Give away list

Edited by mush_monkey (09/28/24 12:44 PM)

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: mush_monkey] * 2
    #28965695 -

Well I've had a pair of trays fully consolidated and cased for about a month with no pinning. So I'm going to try scraping off the casing, overnight soak, and recase tomorrow. Really hope this kick starts growth of i may have to go back to spore print.

Someone please tell me my patience will be rewarded with these :bomb:

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: PsychedelicGator] * 4
    #28965711 -

I've had them take 10 days to pin, and I've had them take 6 weeks. Personally I'd ride it out a little longer.

Edit: since you have two, you could always scrape and soak one. Would be an interesting comparison.


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Edited by thirdeyewild (09/28/24 10:02 PM)

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: PsychedelicGator] * 2
    #28965722 -

PsychedelicGator said:
Someone please tell me my patience will be rewarded with these :bomb:


Your patience WILL be rewarded with these:awesomenod:


--------------------

THE 49TH OYSTER BUCKET TEK

FREE WILL IS A SHADOW

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: thirdeyewild]
    #28965725 -

I cased 2 shoeboxes 14 days ago and I can see primordia forming starting a few days ago.

Do pins take a while to kick off after primordia forms? Or am I close to pins?

Man I wanna eat some semps!

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: PsychedelicGator] * 1
    #28965726 -

Has anyone done that with  Semperviva? I’d leave them be and start another grow. I think my fastest pinning was 18 days and the longest was 42.

Edit:took out a word.

Edited by The Tao (09/29/24 08:58 AM)

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Dkeppel] * 3
    #28965728 -

Should be close to pins. They appear pretty quickly after things knot up.

@Tao I think people were doing that early on in this thread, I haven't done it myself though. Patience is the way :thumbup:


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Edited by thirdeyewild (09/28/24 10:22 PM)

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: thirdeyewild] * 1
    #28965753 -

Sick! Can't wait to see pins. It's a good lesson in patience, I have some zap shoeboxes that are even slower :lol:

I'm surprised how resilient semps seem. I have a bucket which I spawned with bacterial spawn and it's been outside in the lows of 6 to 8C, highs of 16 to 18C, it took 3 weeks but it finally finished colonising and was cased today.

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: PsychedelicGator]
    #28965782 -

PsychedelicGator said:
Well I've had a pair of trays fully consolidated and cased for about a month with no pinning. So I'm going to try scraping off the casing, overnight soak, and recase tomorrow. Really hope this kick starts growth of i may have to go back to spore print.

Someone please tell me my patience will be rewarded with these :bomb:



From the same  ms plate, albeit, different transfer organizations, I've had 10 day pins didnt even get a casing it was so quick. And also +30 days to pin with 2 casings (first colonized completely).

If your casing is "consolidated" try another.  And depending on how dry it is in there maybe a good spritz is needed. If ts pretty moist Maybe it needs more fae.

Since many suggest drier subs are better at the beginning is some what true.  But have had another tub that was about get dumped (and eventually did) Was sus  to begin with. But gave it a good misting as a last chance and couple days later had pins. All aborted and the mist helped the suspect come in to light.

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: PsychedelicGator]
    #28965790 -

From a while back, I remember some people said you just gotta let the surface dry out for a few days and then give it a good misting. Not sure if that still holds true. I had stuff take a few months to get a first flush.


--------------------
Pastywhyte’s Ez dialed monotubs
My Miraculix Potency Test Results
The Hitchhiker's Guide to the Shroomery
My Museum
REV'S Tested: Dehydration temperature vs potency





Edited by Bigworm (09/28/24 11:38 PM)

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Bigworm] * 9
    #28966721 -


the goose droppings 750ml box :lol:

(the ones on top are from mystery swab, what could it be? phobos?)


--------------------
“All this buttoning and unbuttoning”

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 999999999] * 2
    #28967271 -

So I have been doing some workup on a series of Semp plate pin clones. This one was fruiting in plates, and when fruits were transferred whole, more pins erupted from the fruit itself before colonising the agar. Not unlike the Cosmoplex iso of ATL#7 sorta, if you guys remember that one



I grew some more myc from that PTC1 plate and noc'd some jars, which became a sub I made on the 10th September. Here it is today. No casing. Only one I ever had fruit properly without any casing, although whether fruits will grow to usual size remains to be seen



somebody has some similar results already maybe? anybody running plate clones of these regularly? Clones that need no casing?

I have two more plate clones to test which originated from other plates but did not have the cosmoplex style, just solid-sized fruit for a plate. They just got a jar each yesterday and will get more down the line. Just with the potentially long and unknown time line on these cultures and such little room for tubs, I have to go small with the tub size so I am not holding back other things

to find one that reliably does well without a casing would skip the whole step of pasteurization and I really am attracted to that. Curious to hear anyone's thoughts about all this

Do we have a group consensus whether a clone is even useful with Semp at all? Or do you guys feel this is more like Pans where fresh spores from good parent individuals seem to produce better results than most tissue clones?

hope you are all having a good one :cheers:

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 999999999] * 6
    #28967275 -

999999999 said:

the goose droppings 750ml box :lol:

(the ones on top are from mystery swab, what could it be? phobos?)


Could well be Phobos, I thought so from the start but I also have Jack frost that start out looking like Ape and then the blue stays.

Phobos on the other hand went full turtle shell for me hut I definitely had an extreme phenotype.

Phobos



Jack frost



Sorry for the off topic posting.

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: DERRAYLD] * 1
    #28967350 -

glad you shared, D, beautiful turtles!
yeah, the cracked caps lead me to phobos...
They are tiny, but they smell potent (locker room) while drying, gonna run them in a proper thick sub and get to the bottom of it. :rockon:

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 999999999] * 1
    #28967357 -

Right, if they have that swamp water smell then they're phobos.

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: DERRAYLD]
    #28967439 -

I’d like to get a second opinion on this germ plate.  Spores came from a syringe from a trusted vendor.  Every germ plate I’ve done with this syringe has been very bacterial.  When these spots first showed up they looked exactly like bacteria.  Not very white, more of a light milky color that was kind of shiny/reflective.  That classic bacterial look.  Now this morning it appears to be myc.  Does semp myc look suspect when it’s in the early stages of germination? 

I’ve already put some spore solution to a BRF puck to get some myc that I know will be clean but I’m wondering if this is worth trying to transfer/keep around.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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