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PhenoDreamers
hi lol

Registered: 09/02/13
Posts: 3,618
Loc: USA
Last seen: 1 hour, 50 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ambigu]

4
#28942586 - 09/11/24 07:15 AM (2 years, 26 days ago)

Just more aggressive genes. Isolate that..

And for me, I cleaned up Ps. pelliculosa. 11 transfers and only 1 came out worthy enough to continue working with!

Not at home right now and wish I had a picture of the two T1 plates. Will have to share later

Edit:

The T1 plates

Edited by PhenoDreamers (09/11/24 01:23 PM)

Extras:

Scemilope
Probably a Dauterive.

Registered: 11/16/22
Posts: 2,446

Re: AGAR ENVY! (Anything and All things agar!)

[Re: PhenoDreamers]

3
#28942837 - 09/11/24 12:13 PM (2 years, 25 days ago)

I am with Ovoid.

--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWarsIII

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PhenoDreamers
hi lol

Registered: 09/02/13
Posts: 3,618
Loc: USA
Last seen: 1 hour, 50 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Scemilope]

1
#28942859 - 09/11/24 12:41 PM (2 years, 25 days ago)

I have them on substrate now. They should be ready to case soon. Maybe today even,! :smile:

:smile:

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Bigdogg
Stranger

Registered: 06/30/24
Posts: 641
Last seen: 1 year, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Fr3nzy]

2
#28943442 - 09/12/24 02:32 AM (2 years, 25 days ago)

Fr3nzy said:

This is t1, should I do another or is this decent to go to grain?

Take transfers from 3 places to one plate and send that one.

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Headass

Registered: 07/24/24
Posts: 827
Loc: Texas
Last seen: 6 months, 11 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Bigdogg]

3
#28943583 - 09/12/24 07:50 AM (2 years, 25 days ago)

First time using agar, cloning PE, these are about 9 or 10 days, how do they look?

--------------------
Beef’s The Hitchikers Guide to Shroomery
Stipe’s Clean Spawn Checklist
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DV8DUG’s Recognizing Contamination in Grain Jars

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Scemilope
Probably a Dauterive.

Registered: 11/16/22
Posts: 2,446

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Headass]
#28943594 - 09/12/24 08:21 AM (2 years, 25 days ago)

Headass said:

First time using agar, cloning PE, these are about 9 or 10 days, how do they look?

Looking good!
:grampofapproval:

:grampofapproval:

--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWarsIII

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polyflakes
free thinker

Registered: 03/27/08
Posts: 686
Last seen: 24 days, 12 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Scemilope]

2
#28943721 - 09/12/24 11:04 AM (2 years, 25 days ago)

Here is some jedi mind fuck from a year old lc syringe after the first transfer. Just getting back in after a few years.

Also tooth decay same age first plates from syringe growth is a lot slower

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CocaineBuffet
Stranger

Registered: 08/29/19
Posts: 4,504
Last seen: 14 hours, 28 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: polyflakes]
#28944313 - 09/12/24 08:25 PM (2 years, 24 days ago)

LC is looking clean. I would let them go for another week before transferring. Also JMF is probably B+ or GT or any other regular variety

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Radamanto
Stranger

Registered: 08/06/24
Posts: 324
Loc: Spain

Last seen: 10 months, 22 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Bigdogg]

1
#28944644 - 09/13/24 04:30 AM (2 years, 24 days ago)

Radamanto said:

Hello! Noob here on my first time trying agar. Got some growth from golden teacher ms syringe in 1 out of 5 plates. Would love a more experienced opinion of it before doing my first transfer. How does it look? Is it even cube or am I growing a mold ?
Thanks in advance!

Hi! I transferred some wedges from this dish and they are all looking a bit like this one (this was the best looking photo). The original dish is still growing and looking the same, no apparent contams.

How is this transfer looking? Are those things to the left an attempt at rhizomorphic growth?

Any help is appreciated, thank you all!

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PhenoDreamers
hi lol

Registered: 09/02/13
Posts: 3,618
Loc: USA

Last seen: 1 hour, 50 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Radamanto]
#28944680 - 09/13/24 06:03 AM (2 years, 24 days ago)

Yeah it is! Let it grow out a bit and more than likely that's where youll be transferring from next :smile:

:smile:

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haniblecter
Enthusiastic Cuttlefish

Registered: 02/06/14
Posts: 199
Loc: The Mitten
Last seen: 1 year, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: PhenoDreamers]
#28944748 - 09/13/24 06:58 AM (2 years, 24 days ago)

Hello again thread!

I poured 20+ plates of Holy Grail and I'm waiting several days to see if my tek is solid and clean before I do some spore scrapes.

Now, I'm concerned that the 2.5" plates wont be able to show much sectoring before it hits the sides. Is that a valid concern?

I say this because I'm a stones throw from Michigan State, and they have A FUCKING TON of glasswear, and what looks like an infinite supply of sterile styrene plates for sale in a sleeve ($17 bucks per 100, not bad) and I'm getting itchy on going over and buying several hundred.

So, is the benefits of 3.5in plates soo much that I should just abandon HG plates? As a side note, HG plates are pretty good in terms of keeping contams out. I found a 2 year old one with poured agar in it and it had -no- contams present.

--------------------
Laminar Breeze

Filter: 24x24x5.875 Koch
Pre: Dupont Ultimate 9600 14x20x1
Blower: 1TDT2

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Azhiaaziam
Likely Contaminated

Registered: 11/27/23
Posts: 186
Last seen: 8 months, 7 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: haniblecter]
#28944862 - 09/13/24 07:42 AM (2 years, 24 days ago)

When using a spore swab, do you not just rub it on the agar, rotating and zig zagging as you go?

I rolled one pretty good over 5 plates and don't see anything at all in 2-5 days, did I do it wrong?

--------------------
The Hitchhiker's Guide to the Shroomery

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 14,962
Loc: South Africa
Last seen: 1 hour, 56 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Azhiaaziam]

1
#28944867 - 09/13/24 07:44 AM (2 years, 24 days ago)

2-5 days for spore germination?
Be more patient

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LewDoja
mad $cientist, ganjacologist

Registered: 09/18/09
Posts: 4,849
Loc: A fictional tale, in fictional...
Last seen: 4 months, 26 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Azhiaaziam]
#28944872 - 09/13/24 07:46 AM (2 years, 24 days ago)

Like D ^ Said... that's not long at all.

However I've done that same thing before and nada but when the whole tip was cut off and buried in the agar, was successful.

Next time cut the tip off on to the last plate, since it's already been used it's not likely to be able to be used again (cleanly). Or just strands off the main to each plate.

Edited by LewDoja (09/13/24 07:51 AM)

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Azhiaaziam
Likely Contaminated

Registered: 11/27/23
Posts: 186
Last seen: 8 months, 7 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: LewDoja]
#28944876 - 09/13/24 07:48 AM (2 years, 24 days ago)

Awesome, thanks y'all!

--------------------
The Hitchhiker's Guide to the Shroomery

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 14,962
Loc: South Africa
Last seen: 1 hour, 56 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: LewDoja]

1
#28944878 - 09/13/24 07:48 AM (2 years, 24 days ago)

:whathesaid:

Definitely preferred to bury the swab or tulip the swab and transfer a petal rather than using the entire swab.

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Azhiaaziam
Likely Contaminated

Registered: 11/27/23
Posts: 186
Last seen: 8 months, 7 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DERRAYLD]
#28944916 - 09/13/24 08:25 AM (2 years, 24 days ago)

DERRAYLD said:

Definitely preferred to bury the swab or tulip the swab and transfer a petal rather than using the entire swab.

By tulip, you mean spread some petals and rip off the cotton for a bury transfer?

--------------------
The Hitchhiker's Guide to the Shroomery

Extras:

DERRAYLD
Constructus

Registered: 05/13/02
Posts: 14,962
Loc: South Africa
Last seen: 1 hour, 56 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Azhiaaziam]
#28944923 - 09/13/24 08:31 AM (2 years, 24 days ago)

Yes, cut the swab tip into 8 petals which you unfold and remove.
Imagine the swab like a lotus with each petal being a section of the swab.

Extras:

Azhiaaziam
Likely Contaminated

Registered: 11/27/23
Posts: 186
Last seen: 8 months, 7 days

Re: AGAR ENVY! (Anything and All things agar!)

1
#28944971 - 09/13/24 09:12 AM (2 years, 24 days ago)

DERRAYLD said:

Yes, cut the swab tip into 8 petals which you unfold and remove.
Imagine the swab like a lotus with each petal being a section of the swab.

Do you do that with a flamed blade? Doesn't that burn it?

--------------------
The Hitchhiker's Guide to the Shroomery

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Wall.E
Bacteria's Bitch

Registered: 06/05/20
Posts: 3,075
Loc: The Axiom

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Azhiaaziam]

1
#28944977 - 09/13/24 09:15 AM (2 years, 24 days ago)

It burns the edges, but the swab absorbs liquid from the plate and hydrates the spores

--------------------
Mehhh

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