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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #28907242 -

monokaryon ? is that from a single spore being germinated on agar? and dikaryon is ms and then clones from that are still Dikaryon just as a monokaryon can also be fruited and cloned?

hypothetical are taking spore prints from every cap of an f1 grow and mixing it into a single solution and continuing this for several generations?

taking a spore print from each clone and put the spores onto that grain with the clone?

I'm trying to adapt this from a cattle breeding thing I'm working on.

wanting to achieve a "composite" that is "linebred" in a "closed herd" starting with as many "breeds" as possible. adapting the genetics to environment and type .


--------------------
שֶׁהָאָדָם צָרִיךְ לַעֲבֹר עַל גֶּשֶׁר צַר מְאֹד מְאֹד, וְהַכְּלָל וְהָעִקָּר – שֶׁלֹּא יִתְפַּחֵד כְּלָל

"The whole entire world is a very narrow bridge and the main thing is to have no fear at all"-Rebbe Nachman of Breslov

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Re: Ask Quick Questions, Get Quick Answers [Re: sanzer] * 1
    #28907250 -

Yes, a monokaryon is mycelium from a single germinated spore. It is not capable of fruiting - it needs to mate with a compatible monokaryon to produce a fertile dikaryotic culture.

It's not really clear what you're proposing with mixing a spores from a bunch of different mushrooms together. But I suspect the cattle breeding analogy isn't quite relevant, because a single mushroom produces millions of spores and thus millions of genetically distinct potential offspring.

Most named cubensis varieties are pretty stable (each filial generation looks very similar to parents). That implies not very much genetic diversity. So if you're looking to introduce more variation, you would want to start from a wild print or intentionally cross existing varieties.

If you're trying to stabilize a line, the standard practice (as I understand it from reading, not doing) is to clone a specimen with the characteristics/phenotype you want, grow it out, take prints and grow those out, and repeat until each successive generation has the phenotype you want.

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28907253 -

GenesisCorrupted said:
I have a couple jars like that too.
Nothing lost by letting them cook longer.



Sweet thanks geneisis :smile: :hug: I don't mind playing the waiting game to let em all catch up I was just worried 10 or 11 days instead of 7 wouldn't be good for em. I know pinning is something that indicates they want to fruit or something to that effect but it's not pinning yet and it's good to know that even if they do I can let em cook a bit longer


--------------------
R.I.P
Zombi3, Blue Helix
Modest Mouse
Zappa, Jellyfish
Slothie
That Kid With The face
ShLong
Le Canard
split_by_nine
& Big Worm Forever
Etched in the sands of time in the shroomery and ever so beloved and deeply missed by many :heart:

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Re: Ask Quick Questions, Get Quick Answers [Re: sanzer]
    #28907255 -

sanzer said:
monokaryon ? is that from a single spore being germinated on agar? and dikaryon is ms and then clones from that are still Dikaryon just as a monokaryon can also be fruited and cloned?

hypothetical are taking spore prints from every cap of an f1 grow and mixing it into a single solution and continuing this for several generations?

taking a spore print from each clone and put the spores onto that grain with the clone?

I'm trying to adapt this from a cattle breeding thing I'm working on.

wanting to achieve a "composite" that is "linebred" in a "closed herd" starting with as many "breeds" as possible. adapting the genetics to environment and type .




Well I'm not a genetics expert but.

Quote:
ChatGPT

Diakaryon vs mono

"A diakaryon is a type of cell that contains two separate, genetically distinct nuclei within a single cytoplasm. This condition is commonly found in certain fungi, particularly in the life cycle of Basidiomycetes and some Ascomycetes. In these fungi, the two nuclei remain distinct and do not immediately fuse after the fusion of the two cells from different mating types.

On the other hand, a monokaryon is a cell that contains only one nucleus. In the context of fungal life cycles, monokaryotic cells typically occur before the formation of the diakaryon, during the haploid stage after spores have germinated.

In summary:

Diakaryon: A cell with two separate, distinct nuclei.
Monokaryon: A cell with a single nucleus."





Don't put spores directly to grain....

When germinating Ms each little colony (if spread  out enough ) is its own "Strain" isolated.  The practiced methods usually are to start with high MS load to hunt for the phenos you want to keep working with and taking clones of these as well as swabs will help to further isolate those characteristics.

When breeding different "varieties" its often considered stable after f7

Edited by LewDoja (08/11/24 03:28 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #28907258 -

thank you

clarification of my second question. I've seen two methods one with mixing spawn and one with mixing spores ( done a few ways). so my question is say you put 15 varieties of spawn together, or instead of two spore prints together you put 200 spore prints together, and grew this out and kept doing that for each generation.

speeding up of a natural selection process for my environment with a very large genetic pool as the starting point.


--------------------
שֶׁהָאָדָם צָרִיךְ לַעֲבֹר עַל גֶּשֶׁר צַר מְאֹד מְאֹד, וְהַכְּלָל וְהָעִקָּר – שֶׁלֹּא יִתְפַּחֵד כְּלָל

"The whole entire world is a very narrow bridge and the main thing is to have no fear at all"-Rebbe Nachman of Breslov

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Re: Ask Quick Questions, Get Quick Answers [Re: sanzer]
    #28907261 -

They would all just be competing. You wouldn’t really know what you were getting every time. You want to isolate for what you’re actually trying to get. Starting with a huge pool does not sound beneficial to me.

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28907264 -

selecting for survivability to my natural outdoor environment and producing fruits under natural conditions.

starting with a large genetic pool to ensure that possible combinations are not being untested.

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Re: Ask Quick Questions, Get Quick Answers [Re: sanzer]
    #28907266 -

Well, the beautiful part about this community is that a lot of that work has already been getting done.

Depending on your elevation and climate. There are people that are already cultivating outside in the exact same conditions.

At some point, you’re going to get access to the marketplace and then you’ll be able to get genetics from members that have been doing that for years already.

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28907280 -

Sorry another question about dehydrating the mushies. So my question is what's the best way to dehydrate mushrooms I've searched for a few hours on the subject here on the shroomery some goving exact scientific formulas on how pretty much any hot air will make your shrooms lose potency which would make sense why the first one i was looking at doesn't use hot air.

But other people were saying high temps were fine


If the guys science and math was correct I think the first option may be best but I just don't know

(No links)



One of the reviews said it only blows air but not hot air and the dial is a lie but had like 160 other reviews averaged to 4.4 for like 55 bucks if it's safe and can dry em out with regular/warm air and not lose potency I'd prefer it.


And then this is an actual food dehydrator with heat and as a plus I'd be able to make dehydrated apples and such for 60$ but If I lose potency over it I don't want to do that. This is going to be medicine.





--------------------
R.I.P
Zombi3, Blue Helix
Modest Mouse
Zappa, Jellyfish
Slothie
That Kid With The face
ShLong
Le Canard
split_by_nine
& Big Worm Forever
Etched in the sands of time in the shroomery and ever so beloved and deeply missed by many :heart:

Edited by Seriously_trippin (08/11/24 04:55 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Seriously_trippin]
    #28907285 -

In my own experience, it's better to dry them out at a lower temperature, such as 40°C or 35°C. It works well for smaller shrooms, but I couldn't imagine drying a huge chunk like that. It also depends on the humidity in your room.

I'd say that I'd stick with a lower temperature, not more than 40°C, and dry them until they're cracker-dry. Then, storage plays a big role - to avoid moisture, use desiccants, and to avoid oxidation, use oxygen absorbers or infuse the jars with argon or another inert gas. Again, that's how I would do it, just speaking from my personal experience - shrooms dried at 60°C were weaker than those dried at room temperature. If you're about to make dehydrated apples, I'd stick with a food dehydrator, probably, and dry shrooms at the lowest possible temperature. I've never tried a special mushroom dehydrator, so I couldn't say anything about that, but it seems to be pretty much the same as a food dehydrator, just labeled as a mushroom dehydrator.

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Re: Ask Quick Questions, Get Quick Answers [Re: Seriously_trippin]
    #28907286 -

Hi friends, how are we looking here…I’m guessing roughly 4-5 days to go?

Brown rice with agar chunks 31.07

Natalensis



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Re: Ask Quick Questions, Get Quick Answers [Re: Seriously_trippin]
    #28907288 -

Someone mentioned that freeze drying the mushrooms preserves more potency.

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28907290 -

Bra said:
In my own experience, it's better to dry them out at a lower temperature, such as 40°C or 35°C. It works well for smaller shrooms, but I couldn't imagine drying a huge chunk like that. It also depends on the humidity in your room.

I'd say that I'd stick with a lower temperature, not more than 40°C, and dry them until they're cracker-dry. Then, storage plays a big role - to avoid moisture, use desiccants, and to avoid oxidation, use oxygen absorbers or infuse the jars with argon or another inert gas. Again, that's how I would do it, just speaking from my personal experience - shrooms dried at 60°C were weaker than those dried at room temperature. If you're about to make dehydrated apples, I'd stick with a food dehydrator, probably, and dry shrooms at the lowest possible temperature. I've never tried a special mushroom dehydrator, so I couldn't say anything about that, but it seems to be pretty much the same as a food dehydrator, just labeled as a mushroom dehydrator.



Interesting so whatever I choose make sure it doesn't get above 94-104 degrees. I'll look up how many hours  to put it in for . Yeah they probably are the same. I didn't know a out the desiccants and oxygen absorbers though.

I did see a mushroom drying "tub" of sorts with desiccants to dry it no heat or air included maybe I should consider that?

Nah sounding like from your description I should just pick one and set it as low as possible

GenesisCorrupted said:
Someone mentioned that freeze drying the mushrooms preserves more potency.



I saw that I'd looooove a freeze drier but they are pretty expensive

What would u do genesis?


--------------------
R.I.P
Zombi3, Blue Helix
Modest Mouse
Zappa, Jellyfish
Slothie
That Kid With The face
ShLong
Le Canard
split_by_nine
& Big Worm Forever
Etched in the sands of time in the shroomery and ever so beloved and deeply missed by many :heart:

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Re: Ask Quick Questions, Get Quick Answers [Re: Seriously_trippin]
    #28907291 -

I use a quite small dehydrator.
I intend to get a nice freeze dryer at some point but for now I don’t see an issue just throwing it into the dehydrator for about 12 hours.

Mine doesn’t go up that high, but everything comes out cracker dry.

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28907294 -

Oh
GenesisCorrupted said:
I use a quite small dehydrator.
I intend to get a nice freeze dryer at some point but for now I don’t see an issue just throwing it into the dehydrator for about 12 hours.

Mine doesn’t go up that high, but everything comes out cracker dry.



Hells yeah I'll get a normal food dehydrator then and I can make my apple chips ^^ it does say you can set it to 95-104 degrees.


--------------------
R.I.P
Zombi3, Blue Helix
Modest Mouse
Zappa, Jellyfish
Slothie
That Kid With The face
ShLong
Le Canard
split_by_nine
& Big Worm Forever
Etched in the sands of time in the shroomery and ever so beloved and deeply missed by many :heart:

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Re: Ask Quick Questions, Get Quick Answers [Re: Seriously_trippin] * 1
    #28907300 -



--------------------
𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

Edited by Tri (08/11/24 06:30 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: salubrious]
    #28907301 -

salubrious said:
Hi friends, how are we looking here…I’m guessing roughly 4-5 days to go?

Brown rice with agar chunks 31.07

Natalensis







Not really seeing anything that screams needing to wait 4-5 days.
Unless I'm missing something, those look about ready to shake and spawn  if recovery is quick.

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Re: Ask Quick Questions, Get Quick Answers [Re: Tri] * 2
    #28907302 -

Tri-Polar said:

https://www.shroomery.org/forums/dosearch.php?forum%5B%5D=f2&words=Dehydrate&namebox=&expert=1&replybox=&how=all&where=body&tosearch=both&newerval=6&newertype=y&olderval=&oldertype=y&minwords=&maxwords=&limit=25&sort=r&way=d

The consensus from TCs has generally been to dry them hot and fast to lock in potency



:whathesaid:  I almost always tick that last box "show 1 resut per thread"

The degradation of the active alkaloids is from oxidation in aqueous solution....or similarly. So removing water quickly, helps limit the negative effects of oxygen. Though I suppose heat will damage it not just oxidize it (thermal degradation.) it's pretty safe within the limits of retail dehydrators


Edit:
Don't forget the diy toggle switch , to cut power to the heating element.


--------------------
A_wise_man said:
"Bad drugs tell you, that you want more;
  Good drugs tell you, that you've had enough"



🎶  KIND TC's - by: Tri  🎶

Edited by LewDoja (08/11/24 03:26 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #28907305 -

LewDoja said:
salubrious said:
Hi friends, how are we looking here…I’m guessing roughly 4-5 days to go?

Brown rice with agar chunks 31.07

Natalensis







Not really seeing anything that screams needing to wait 4-5 days.
Unless I'm missing something, those look about ready to shake and spawn  if recovery is quick.



Thanks Lewdoja…much appreciated

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Re: Ask Quick Questions, Get Quick Answers [Re: Tri]
    #28907310 -

Tri-Polar said:


https://www.shroomery.org/forums/dosearch.php?forum%5B%5D=f2&words=Dehydrate&namebox=&expert=1&replybox=&how=all&where=body&tosearch=both&newerval=6&newertype=y&olderval=&oldertype=y&minwords=&maxwords=&limit=25&sort=r&way=d

The consensus from TCs has generally been to dry them hot and fast to lock in potency





Damn that's a lot to chew on. That logic makes sense but then ya got newer growers leaning towards 95 -104 makes it hard to know, but one of those threads in particular I'll read again tomorrow thanks. Just makes me even more confused as to who's right but that guy had a strong argument


--------------------
R.I.P
Zombi3, Blue Helix
Modest Mouse
Zappa, Jellyfish
Slothie
That Kid With The face
ShLong
Le Canard
split_by_nine
& Big Worm Forever
Etched in the sands of time in the shroomery and ever so beloved and deeply missed by many :heart:

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Shop: Mushroom-Hut Mono Tub Substrate   Sporeworks.EU Spores for European Microscopy   MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale


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