OldManRiver said:

AspectOfTheCreator said:

My t1 plates grow out clean. Is it risky or bad practice to use these for inoculating grain rather than going to t2 first?

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I'll use them without concern.  I know that my transfer process is clean.  If you are confident that your transfers are reliable, I wouldn't hesitate. 

If the original plate was gross, I'd do a T2.

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MasterNeo said:

Check out the aerial rope a dope going on right here

Storm Troopers T2. Really happy with the way it decided to do it's thing, & it's so fresh, & so clean

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SWBZA said:

Hi all.

First time making agar plates with yeast: 2% agar, 1.6% LME, 0.2% nutritional yeast.  Same process followed for all my other agar - slowly vent PC, sterilise for 20 minutes, let cool down and pour at 50 degrees celsius.  Besides the plates looking butt ugly, today (day 4), my plates look some variation of this:



That looks very suspicious to me   Is this what yeast plates are supposed to look like?

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scwoopz said:

here’s some T2 plates. What do you think is up with the denser ring of mycelium that all of the plates have near the outer edge? Do these look healthy?

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SWBZA said:

Interesting post by SPG. Thanks for the pointer. I'll give my next batch a longer cook. I took the time for a recommendation to balance sterility against caramelization for LCs. I'm going to have to go back to my notes - getting confused working from memory, I think. Okay, suckers, next time I'm sterilising the living daylights out of you, little bugs! Anyway, don't think I'll do yeast plates again anytime soon. They look ugly!

Edit: just checked the SPG formula: 32 minutes for me.  So plates probably not all clean.  Bummer.

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Now we wait and see.  Hopefully I'll be able to declare clean agar in about a week.

scwoopz said:

here’s some T2 plates. What do you think is up with the denser ring of mycelium that all of the plates have near the outer edge? Do these look healthy?

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SwabMarley said:

Been 17 days so far. Yeah it’s very slow. I think this appeared around day 6ish. I will hold on to it for another week or so. If not I think there’s juuuust enough left on the print to have another go.

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SWBZA said:

Hi all.

First time making agar plates with yeast: 2% agar, 1.6% LME, 0.2% nutritional yeast.  Same process followed for all my other agar - slowly vent PC, sterilise for 20 minutes, let cool down and pour at 50 degrees celsius.  Besides the plates looking butt ugly, today (day 4), my plates look some variation of this:



That looks very suspicious to me   Is this what yeast plates are supposed to look like?

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GenesisCorrupted said:

I go up to an hour on liquid culture.
I go like 50 to 55 minutes on Petri dishes too.

I just cover the jars with foil, so water doesn’t get into the filters.
But why not?

How long is too long to sterilize?

I feel like I shouldn’t have to go past an hour.

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Nichrome said:

SWBZA said:

Hi all.

First time making agar plates with yeast: 2% agar, 1.6% LME, 0.2% nutritional yeast.  Same process followed for all my other agar - slowly vent PC, sterilise for 20 minutes, let cool down and pour at 50 degrees celsius.  Besides the plates looking butt ugly, today (day 4), my plates look some variation of this:



That looks very suspicious to me   Is this what yeast plates are supposed to look like?

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That is bacteria. Toss all plates that look like that right in the trash. Some kind of flaw in your method.

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