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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28650611 -

Yahra said:
I need to test my new pc but i am not ready to go in full operation mode yet.

Thought i could prepare some agar for testing.

Are there any disadvantages if i prepare agar and let it sit in the fridge for a couple of weeks before i use it compared to pour it "fresh"?



For two weeks I honestly wouldn't bother with the fridge unless you poor the minimal possible amount to coat the bottom.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: elasticaltiger]
    #28650618 -

I would prepare two 250ml media bottles with 400ml water, 9 grams agar and 6 grams BRF.

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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra]
    #28650630 -

Oh sorry, i missed the part about not pouring. So if you PC it and then put the bottle in the fridge it's going to turn into a giant block of solid agar. Is the plan to microwave it back up to temp for pouring?


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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Re: Ask Quick Questions, Get Quick Answers [Re: elasticaltiger]
    #28650652 -

Yes.

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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 2
    #28650660 -

The only disadvantage I can really think of is the bottle picking up some contamination from the fridge on the exterior which could fall into your dishes when you pour. Do you have a fresh ziplock that can hold the media bottle? Fridges are pretty gross unless they are dedicated to mycology.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: arnold582] * 1
    #28650755 -

arnold582 said:
During the first flush I accidentally waterlogged my substrate. Once I dialed back on misting and FAE, large parts of the tub were overgrown with cotton-like growth.

At first I thought it might be mold but it's been snow white like this for around a week now. Is this likely just odd mycelium growth caused by insufficient FAE or could it be something else?






Bump :tongue: I've seen photos of "fuzzy feet" before, but this is an extreme version of that, the fuzz was super thick (5mm on some fruits) and I'm still trying to figure out why. Can an overly wet substrate (or another environmental condition) cause this, or can I safely assume it was just FAE?

Most of those fruits from would-be 2nd flush ended up aborting after my careless misting.

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Re: Ask Quick Questions, Get Quick Answers [Re: arnold582]
    #28650756 -

As long as I see droplets of water on the sides of my mono tub, I shouldn’t spray it all right?

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28650765 -

I'm probably the worst person to speak up :lol: but I think it's more complicated than that. There've been times where my surface was dry (according to other members here) even though my tub walls were dripping wet.

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28650767 -

GenesisCorrupted said:
As long as I see droplets of water on the sides of my mono tub, I shouldn’t spray it all right?



The sub is going to sweat allot causing those droplets on the side.

What does the actual surface look like?


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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Re: Ask Quick Questions, Get Quick Answers [Re: elasticaltiger]
    #28650870 -

I’m not even to the stage yet. I just want to know when I should spray. I was told never to spray directly on the fruit.
So I would have no choice but to spray the walls and the roof of the tub, right?

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted] * 2
    #28650886 -

GenesisCorrupted said:
I’m not even to the stage yet. I just want to know when I should spray. I was told never to spray directly on the fruit.
So I would have no choice but to spray the walls and the roof of the tub, right?



You spray the surface of the sub until your pins turn into "fruits". After that they'll be sweating so much they'll be holding surface conditions without any assistance from you.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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Re: Ask Quick Questions, Get Quick Answers [Re: elasticaltiger]
    #28650973 -

Good evening.
I work a bunch, and was wondering if the light schedule is precise like cannabis and other photoperiod sensitive plants (Papaver as well).
I like to look at them before I go to work, and I do work after I come home like pressure cooker shit, or looking(I'm good at that!).
Would intermittent 12h be ok? A few hours in morning and some in evening?
Dank you

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28650977 -

Smellyhobbit said:
Put in 400ml each in 3 jars for agar broth and got out 350ml after the PC cycle. Water was colored. This didn’t happen for the first year and a half of making agar, but now it seems like a consistent outcome.

Why is that?




Media bottle lids get warped over time. Tighten them a little more.

Also if the media bottle is all that's in the pc you can do a shorter vent. Often when I have escapage it is due to too high of heat or too lengthy of a venting process. The stuff in the bottle boils over and escapes. Try not venting for as long or venting at a lower starting heat.

I see you said "jars" though so probably not the lids unless they're plastic, then the same goes.


--------------------
Question everything, it's very responsive.


Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted] * 1
    #28650983 -

GenesisCorrupted said:
I’m not even to the stage yet. I just want to know when I should spray. I was told never to spray directly on the fruit.
So I would have no choice but to spray the walls and the roof of the tub, right?



Some fruits enjoy a good drench.

Playa for example likes a good heavy rain at about half height.

As long as the surface looks good it's safer not to spray. Just don't worry about it too much either way. Even if it's way too dry for some days a good spray turns that right around. You'll know better what to do as you watch it unfold. Unless it is too wet and soppy a small spritz right on the mush will do no harm.


--------------------
Question everything, it's very responsive.


Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 1
    #28650996 -

Nichrome said:
Smellyhobbit said:
Put in 400ml each in 3 jars for agar broth and got out 350ml after the PC cycle. Water was colored. This didn’t happen for the first year and a half of making agar, but now it seems like a consistent outcome.

Why is that?




Media bottle lids get warped over time. Tighten them a little more.

Also if the media bottle is all that's in the pc you can do a shorter vent. Often when I have escapage it is due to too high of heat or too lengthy of a venting process. The stuff in the bottle boils over and escapes. Try not venting for as long or venting at a lower starting heat.

I see you said "jars" though so probably not the lids unless they're plastic, then the same goes.



I DID say jars, but this most silly of geese meant media bottles.  Thanks for the advice I’ll give that a go. I vent with the burner up all the way and it never occurred to me that I definitely don’t need to do that.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #28651063 -

Yeah running on high is a bad habit for sure. Learned this the hard way with spawn bags. Now I feel like I can cook anything in the pc with low heat 😂

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Re: Ask Quick Questions, Get Quick Answers [Re: PhenoDreamers]
    #28651085 -

Im new to agar but not new to growing. My first 10 dishes I poured where clumpy, filled with bubbles and disasterous, since then no dramas. I noc'd my first lot of dishes with spore syringe last week, all are going well. Problem is I had some spare dishes, im 98% sure I didnt get them anywhere near my SAB, but one plate looks like this...


SO my question is, is this MYC and ive inoculated it but discarded it and forgot, or have aI just got a conventient looking mould growth. THe dish has been sitting on our dining table for the past week unwrapped. I just wrapped it today... everything is telling me its not MYC....

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Re: Ask Quick Questions, Get Quick Answers [Re: seifer]
    #28651086 -

That looks like a pretty healthy genetic of myc to me

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28651107 -

GenesisCorrupted said:
That looks like a pretty healthy genetic of myc to me



Me too, I just cant work out how it came to be hence im thinking it cant possibly be that....

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Re: Ask Quick Questions, Get Quick Answers [Re: seifer]
    #28651116 -

Looks like there’s a spot where a drop fell onto it. You probably just knocked it and forgot.

I noticed your bubbles.
What kind of mix do you use?
I make my own PDA.
I’ve never had an issue with bubbles before.
If anything, I tend to go on the more stiff side. So mine tends to be almost a little too dry. But my genetics seem to like it.

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Shop: MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Autoflowering Cannabis Seeds   Sporeworks.EU Spores for European Microscopy


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