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Offlinestarburstmemories
Lychen
I'm a teapot


Registered: 10/23/23
Posts: 77
Last seen: 9 days, 3 hours
Re: Ask Quick Questions, Get Quick Answers [Re: arnold582] * 1
    #28648321 - 02/06/24 12:57 AM (3 months, 9 days ago)

Quote:

arnold582 said:
2 weeks ago, I started another jar using a MP filter, but this time around I changed it after PC'ing. I thought this might solve the gas exchange issue (which I thought stemmed from gummed up tape pores), but just like my previous batch, this one stalled out after shaking without any visible myc growth after ~4 days. I was surprised because I had really strong mycelium growth before (sadly no pics).

At first, I thought the jar must've been contaminated, even though there were no signs of it other than lack of myc growth, but then I carefully loosened the lid inside my SAB and 24h later, I have visible mycelium growth again.

Would it be fair to conclude that this is almost certainly a gas exchange issue?




I would say so, yes. I've had the exact same situation happen to me with too little gas exchange. Popping the lid produced myc the next day. So I concluded same as you :smile:

That being said, changing your filter, whether it's MP, polyfill, paper sticker, is not a good idea. It's supposed to be sterilised with the jar and not tampered with afterward. I did replace paper filters with sterilised polyfill, in my sab, but it's definitely not recommended.

Also, 4 days is a little early to draw conclusions. I've had jars take 1-2 week before showing any sign of colonisation.

Quote:

arnold582 said:
I'm not sure if my gas exchange hole is too small (3mm or 1/8"), or if this MP tape is just ass. It's not 3M branded, mind you, but it's supposed to be nearly identical, it's described as surgical, paper-based, breathable tape.




I'm not sure about the MP you use, but when I switched from polyfill to paper filters, keeping one 10mm hole was way insufficient. Super slow colonisation, popping the lids needed.

Here I'm testing my proven polyfill setup against 2x10mm paper filter holes, on 2 strains.

I don't know what size your jars are, but 3mm could sound a wee small :zoom:



Good luck with your new lid! :peace:


--------------------


Reaching for the summit, one way or another :leaving:



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OfflineDERRAYLD
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Re: Ask Quick Questions, Get Quick Answers [Re: starburstmemories] * 1
    #28648336 - 02/06/24 01:33 AM (3 months, 9 days ago)

Just FYI,  mp tape has to be 4 layers minimum or it's just letting contam in due to the large pore size of mp tape.

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InvisibleSWBZA
Male


Registered: 05/09/23
Posts: 207
Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #28648413 - 02/06/24 03:39 AM (3 months, 9 days ago)

Quote:

DERRAYLD said:
Just FYI,  mp tape has to be 4 layers minimum or it's just letting contam in due to the large pore size of mp tape.



After 4 layers, though, is there any FAE possible still? Sounds like MP has no place in our hobby other than for covering the holes in a modified monotub?

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Offlinechris77
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Registered: 01/17/22
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Re: Ask Quick Questions, Get Quick Answers [Re: SWBZA]
    #28648435 - 02/06/24 04:14 AM (3 months, 9 days ago)

Quote:

SWBZA said:
Quote:

DERRAYLD said:
Just FYI,  mp tape has to be 4 layers minimum or it's just letting contam in due to the large pore size of mp tape.



After 4 layers, though, is there any FAE possible still? Sounds like MP has no place in our hobby other than for covering the holes in a modified monotub?




4 layers of mp are safe and work very well


--------------------
the observer is the observed
            j. krishnamurti

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InvisibleSWBZA
Male


Registered: 05/09/23
Posts: 207
Re: Ask Quick Questions, Get Quick Answers [Re: chris77]
    #28648442 - 02/06/24 04:21 AM (3 months, 9 days ago)

Quote:

chris77 said:
Quote:

SWBZA said:
Quote:

DERRAYLD said:
Just FYI,  mp tape has to be 4 layers minimum or it's just letting contam in due to the large pore size of mp tape.



After 4 layers, though, is there any FAE possible still? Sounds like MP has no place in our hobby other than for covering the holes in a modified monotub?




4 layers of mp are safe and work very well



Thank you.

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Invisiblearnold582


Registered: 10/30/23
Posts: 89
Loc: Mars
Re: Ask Quick Questions, Get Quick Answers [Re: starburstmemories] * 1
    #28648514 - 02/06/24 06:40 AM (3 months, 9 days ago)

Thanks! Changing the filter after sterilization was risky for sure, but I wanted to try it, for science :awesome:

It seems odd that a 3mm hole wouldn't provide enough gas exchange for a 1L jar that was only 25% colonized at the time, and it was only 2 layers of tape. I have the same size hole with the new ez felt filters too, so I should know soon enough.

This jar is almost fully colonized now (day 18), and with proper gas exchange, I think it would've been done a few days ago.


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OfflineDERRAYLD
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Re: Ask Quick Questions, Get Quick Answers [Re: arnold582] * 1
    #28648525 - 02/06/24 06:53 AM (3 months, 9 days ago)

You guys are making some major assumptions, unless you have a sealed jar or are using a syringe filter it is incredibly unlikely it is a slow down to to GE.

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Invisiblearnold582


Registered: 10/30/23
Posts: 89
Loc: Mars
Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #28648534 - 02/06/24 07:03 AM (3 months, 9 days ago)

Quote:

DERRAYLD said:
You guys are making some major assumptions, unless you have a sealed jar or are using a syringe filter it is incredibly unlikely it is a slow down to to GE.




Hmm, I'm new but so far, I've prepared 6 jars using 2 layers of that off-brand MP tape as a filter. They all progressed quite slowly and stalled after shaking at around 30%. The first time around I left them alone for over a week with no signs of recovery, this time it was 4 days, but they all came back strong within 24h of loosening the lid.

I had a successful grow using those first 4 jars without contamination, so I'm not sure what else it could be?

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OfflineDERRAYLD
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Re: Ask Quick Questions, Get Quick Answers [Re: arnold582] * 2
    #28648540 - 02/06/24 07:09 AM (3 months, 9 days ago)

Define successful?

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Invisiblearnold582


Registered: 10/30/23
Posts: 89
Loc: Mars
Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD] * 1
    #28648548 - 02/06/24 07:16 AM (3 months, 9 days ago)

It's made it to 2nd flush so far without contamination, I ended up with around 50g dry after the first flush using approx. 2L of spawn and 3L of coir.

I think it has something to do with this brand of MP tape specifically (unfortunately I can't share the name of it, it's a small regional brand). Could anything other than lack of GE cause a jar to completely stall and then suddenly come back when the lid is loosened?

Edited by arnold582 (02/06/24 07:17 AM)

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OfflineSkropi
Space Ritual
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Registered: 11/01/23
Posts: 414
Loc: Greece
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Re: Ask Quick Questions, Get Quick Answers [Re: arnold582]
    #28648561 - 02/06/24 07:27 AM (3 months, 9 days ago)

I don't know what is considered successful, but my instinct says that 2 litres of spawn should produce much more than that. I may be totally wrong of course, I just judge by the amount of "spawn" in my BRF cakes and how much they have produced till now. Again, take this with a lot of grains of salt.


--------------------
Ἄνδρα μοι ἔννεπε, Μοῦσα, πολύτροπον

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Invisiblearnold582


Registered: 10/30/23
Posts: 89
Loc: Mars
Re: Ask Quick Questions, Get Quick Answers [Re: Skropi]
    #28648565 - 02/06/24 07:32 AM (3 months, 8 days ago)

I bet, but I also messed up a lot during the fruiting stage, it was my first time working with bulk and I've only done PF Tek once before. I accidentally dried the substrate out, then waterboarded it, undermisted, overmisted, too much FAE & too little. I'm still trying to dial in.

Anyhow I'm here to learn so if anyone has an alternative explanation for my stalling problem that goes away with a loose lid, I'd love to hear it. I had a dedicated thread a couple of months ago and the prevailing opinion was lack of gas exchange.

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InvisibleEniQma

Registered: 11/28/23
Posts: 679
Re: Ask Quick Questions, Get Quick Answers [Re: arnold582]
    #28648569 - 02/06/24 07:35 AM (3 months, 8 days ago)

QQ:
How long will properly dried mushrooms stay consumable in the plastic ziplocks sandwich baggies that I gave to my friends and family for Christmas? No desiccant packets and not the kind that slide shut.


--------------------
The wild nature of the ovoids being from the same part of the world as me makes me feel like the relationship isn’t founded upon deception (indoor cultivation of non native species through simulation) and has a healthier symbiotic benefit.

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InvisibleSwabMarley
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Registered: 12/07/20
Posts: 1,571
Loc: Drunken Stupor
Re: Ask Quick Questions, Get Quick Answers [Re: EniQma]
    #28648574 - 02/06/24 07:39 AM (3 months, 8 days ago)

Quote:

EniQma said:
QQ:
How long will properly dried mushrooms stay consumable in the plastic ziplocks sandwich baggies that I gave to my friends and family for Christmas? No desiccant packets and not the kind that slide shut.



Not too long. 6 months tops I’d say

Edit: ah you said consumable, if they get them transferred over to a non porous container with desiccants then it might save them and they may be good to go for maybe a year or more.

If the stalks start to go bendy then I would just advice them to make a tea out of them.

Edited by SwabMarley (02/06/24 07:41 AM)

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InvisibleEniQma

Registered: 11/28/23
Posts: 679
Re: Ask Quick Questions, Get Quick Answers [Re: SwabMarley]
    #28648592 - 02/06/24 07:52 AM (3 months, 8 days ago)

That’s good advice. Thank you fam

Is 20 days from spawn to harvest at 1:2 a pretty fast cube culture?
Room temps between 70-75


--------------------
The wild nature of the ovoids being from the same part of the world as me makes me feel like the relationship isn’t founded upon deception (indoor cultivation of non native species through simulation) and has a healthier symbiotic benefit.

Edited by EniQma (02/06/24 08:05 AM)

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Offlinetree frog
eats bugs
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Registered: 09/14/23
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Re: Ask Quick Questions, Get Quick Answers [Re: EniQma]
    #28648629 - 02/06/24 08:14 AM (3 months, 8 days ago)

Anyone know of a variety called 'Peace Train' and what its genetics might be?  My sister was getting it from a friend (it's a cube and possibly some marketing hype).

Anyway, trying to figure out how potent it might be (if it has PE genetics or not, basically), so I can explain better to her how potent Pans are.

nm:  Looks like marketing hype.  Faht's posted the varieties link in another thread and peace train isn't on it.

Edited by tree frog (02/06/24 08:23 AM)

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Offlinejimineycricket
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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #28648659 - 02/06/24 08:26 AM (3 months, 8 days ago)

So it seems the fashion these days with the old pf tek is to abandon the SGFC and shred those little cakes into pasteurised coir.

Bigger yields and easy set and forget shoeboxes being the reasoning.

But how much larger are the yields really? Is it really worth it to shred cakes?

Could one fruit in an sgfc and them after a flush or 3 shred to Coir to squeeze out the last bit of fruit?

Thanks folks

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InvisibleSwabMarley
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Re: Ask Quick Questions, Get Quick Answers [Re: EniQma]
    #28648664 - 02/06/24 08:30 AM (3 months, 8 days ago)

Quote:

EniQma said:
That’s good advice. Thank you fam

Is 20 days from spawn to harvest at 1:2 a pretty fast cube culture?
Room temps between 70-75



20 days from spawn to harvest is indeed pretty fast! Reserved for your standard cubes and in my experience some fast pan varieties that will obliterate a sub in like 3-4 days. You case and then have fruits in like 8-10 days. As always, it’s massively dependant on, in no particular order;
1. What strain/variety you’re working with.
2. The genetics of said strain/variety
3. Environmental factors
4. Health of culture
5. The speed of light in a vacuum
6. Whether the mushroom gods think you’re a bit of a dick or not

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Offlinetree frog
eats bugs
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Registered: 09/14/23
Posts: 1,556
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Re: Ask Quick Questions, Get Quick Answers [Re: SwabMarley]
    #28648665 - 02/06/24 08:32 AM (3 months, 8 days ago)

Some pan species will show fruits a few days after casing.  Pan Cyan (TT)BVI put out two flushes in ten days (from being cased) in a 75 degree chamber.

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InvisibleSwabMarley
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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #28648677 - 02/06/24 08:39 AM (3 months, 8 days ago)

Quote:

tree frog said:
Some pan species will show fruits a few days after casing.  Pan Cyan (TT)BVI put out two flushes in ten days (from being cased) in a 75 degree chamber.



Loving the use of brackets! Never ran (TT)BVI before now but it is FAST! I can’t remember how fast my BVI were but I do remember them being pretty fuckin quick if memory serves which would make sense.

Spawn to harvest in 20 days for a cube is on the very fast side of things. Not ran a massive amount of ‘standard’ cubes as I used to like to run PE, APE or any variation thereof. As a benchmark I would say from spawn to harvest anywhere from 24(ish) days would be normal.

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