Spacecadet94 said:

What causes blobbing and how do we fix it? Is it because we need p-casing layer or is it genetics? Spawn ratio? Ive heard alot of things. Chime in!

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tryptkaloids said:

Aspect, try to pour some hot agar over a culture the size of a nickel, only take from the top layer when transferring.

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Smellyhobbit said:

Nah it’s not an age thing. Looks like goofy ahh genes or possibly bacterial spawn. Did you notice any signs of bacteria in your spawn? Might just work with a different culture if that one isn’t performing.

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Kinoko314 said:

Your jar didn't look particularly bacterial, but you aren't growing mold either.  You wouldn't have mushrooms if it was.

A hot pour may even be considered a step back from water agar, unless you hot pour water agar.  (I just thought of that, and it's perfect!  Combining both techniques should work great.)  Generally it's more of a race.  As soon as myc breaks the surface you want to transfer it.

I don't think your issues are likely to be from contamination though.  It's hard to say what the problem is, but I feel pretty confident that the problem isn't contamination.  Stipe is probably on to something.  Try a slightly higher ratio and use a well compressed pseudo-casing, and maybe you'll have better luck.

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AspectOfTheCreator said:

What about when the culture looks clean but still leads to funky jars/tubs? Theres many posts on here about enmeshed mold/bacteria on cultures that otherwise look clean.

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Nonagon said:

Do people sterilize tools inside their SAB when using non-torch related methods - Heating coils and such?

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AspectOfTheCreator said:

What about when the culture looks clean but still leads to funky jars/tubs? Theres many posts on here about enmeshed mold/bacteria on cultures that otherwise look clean.

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My opinion, operator error. I grow many different bacteria and molds on purpose as well as countless varieties of mushroom mycelium. It's extremely rare to find mold of any kind "hiding" or "enmeshed" in with mycelium from the genus Psilocybe. It is typically visible and almost always causes a reaction of some sort. Bacteria infections or infestations are typically also visible.

Enmeshed mold and bacteria are often touted but in my opinion lack any merit and should stop being parroted all the time. Unpopular opinion I know I know.

What it boils down to is weak genetics and susceptibility, and possibly or even very likely mycopsychology. Is there strife within the colony? Is the colony always trying to eject an outcast like those cultures that never stop scalloping? Black sheep syndrome leads to green tubs. Will to live is important. Really there is no way to nail down a 1+1=2 scenario with mushrooms. There's too much ∞/1 going on.

Hope that helps....

Way said:

Yeah but every time those walls flex it's creating waves of air, isn't it?

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