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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #28503849 -

Patience. It should pin. What variety? My bet is genetics


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #28503850 -

Cambodian, these actually colonized pretty quickly in the jars. About 2 weeks or a little less with 1 cc lc each.


BeefSupreme, thanks for the boost. I left you a nice review too.

Edited by Sauceman77 (10/14/23 12:28 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Sauceman77]
    #28503854 -

Yeah brother just let them vibe. It will fruit when it wants to.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28504202 -





I'm trying to get more clear plates if anyone can point me in the right direction? I finally got the condensation taken care of, I pour at about 95-100F, only condensation on top plates, goes away after a few days in the SAB. At 2% mix, I premix in pot, bring to boil while stirring, this usually gives a consistent fluid, then I transfer to media bottles, PC for 45 mins at 15 psi, cool and pour. Could they be carmelizing during PC process? I've been thinking of doing 25~ mins and seeing what that does. I also got some different LME and Agar, but the ones I used prior are pretty standard around here. I'm not opposed to a hot plate/magnetic stirrer, I just want crystal clear plates like I've been seeing. TIA. Cheers!


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How would you feel if you hadn't eaten breakfast?

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Re: Ask Quick Questions, Get Quick Answers [Re: FeelFighters] * 1
    #28504205 -

FeelFighters said:




I'm trying to get more clear plates if anyone can point me in the right direction? I finally got the condensation taken care of, I pour at about 95-100F, only condensation on top plates, goes away after a few days in the SAB. At 2% mix, I premix in pot, bring to boil while stirring, this usually gives a consistent fluid, then I transfer to media bottles, PC for 45 mins at 15 psi, cool and pour. Could they be carmelizing during PC process? I've been thinking of doing 25~ mins and seeing what that does. I also got some different LME and Agar, but the ones I used prior are pretty standard around here. I'm not opposed to a hot plate/magnetic stirrer, I just want crystal clear plates like I've been seeing. TIA. Cheers!


I only PC agar 30 min and I also only use 1% LME 1.5% agar Also Lovibond 4 LME will make darker agar than the preferred Lovibond 2

Edited by RockinRobot (10/14/23 12:45 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: FeelFighters] * 1
    #28504213 -

I just slowly stir in my powder then pc.  Helps with sediment. As far as perfectly transparent, I can't help you. My plates always turn out a little opaque


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: FeelFighters] * 3
    #28504305 -

FeelFighters said:




I'm trying to get more clear plates if anyone can point me in the right direction? I finally got the condensation taken care of, I pour at about 95-100F, only condensation on top plates, goes away after a few days in the SAB. At 2% mix, I premix in pot, bring to boil while stirring, this usually gives a consistent fluid, then I transfer to media bottles, PC for 45 mins at 15 psi, cool and pour. Could they be carmelizing during PC process? I've been thinking of doing 25~ mins and seeing what that does. I also got some different LME and Agar, but the ones I used prior are pretty standard around here. I'm not opposed to a hot plate/magnetic stirrer, I just want crystal clear plates like I've been seeing. TIA. Cheers!


using distilled water helped my plates notably

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Re: Ask Quick Questions, Get Quick Answers [Re: normalperson]
    #28504344 -

I'm going to give the distilled water a shot.  Not the original questioner but was following with interest.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 1
    #28504457 -

I use distilled water 1.5% agar/LME and pc 20 minutes. I dont swirl the bottle after sterilization but the early plates are glass clear and the last 3 or 4 are always speckled with carmelized LME (assuming thats what it is). Dont think its possible to get them all crystal clear using LME unfortunately.

Got a question ive never seen asked. I have a clone culture going. Theres 1 small contamination on the edge. Rather than transferring the myc, I transferred out the contamination. Is this likely to save the plate? Got a bunch of other plates so its not too important just more of a test. Never heard of anyone doing that.


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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator]
    #28504459 -

I've seenit done, but I'd still take it to a new plate before grain


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #28504521 -

I just got some clean syringes of Enigma and Jack Frost liquid culture. How many CC should I use for a pint of LC so I can expand this culture? Just a drop should do it no??? Also I want to do the same with Agar. Is there a easy way to get just a drop out of the syringe without using the plunger?


--------------------
:rockman::rockman:
๐Ÿ„[CLICK] Grow Journal ๐Ÿ„

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Re: Ask Quick Questions, Get Quick Answers [Re: DoomShroom420]
    #28504536 -

Little millet question update.

I did a test jar of WBS using the same method from the other jars so I could cut some grains open and see how hydrated they were. Every grain was picture perfect, hydrated all the way through.

So if the millet is properly hydrated, what would cause myc to eat it so so slowly?


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: DoomShroom420]
    #28504541 -

DoomShroom420 said:
I just got some clean syringes of Enigma and Jack Frost liquid culture. How many CC should I use for a pint of LC so I can expand this culture? Just a drop should do it no??? Also I want to do the same with Agar. Is there a easy way to get just a drop out of the syringe without using the plunger?


Yes, one drop for the new lc is sufficient. One drop can be a lot for an agar plate. Press the plunger lightly and a drop will start to form. Catch that with an inoculation loop and streak the plate.

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Re: Ask Quick Questions, Get Quick Answers [Re: DoomShroom420] * 1
    #28504544 -

DoomShroom420 said:
I just got some clean syringes of Enigma and Jack Frost liquid culture. How many CC should I use for a pint of LC so I can expand this culture? Just a drop should do it no??? Also I want to do the same with Agar. Is there a easy way to get just a drop out of the syringe without using the plunger?


I wouldn't expand it directly to more lc. Put it to agar and expand it that way, use the rest of the lc on grain or cakes

It's best to avoid liquids until you get a feel for agar, how it should look, and get a few bulk grows under your belt before attempting to make your own lc, especially with untested innoculant


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #28504561 -

Ok, so forgetting LC altogether and will just worry about Agar. That does make things less stressful and ease the costs right now. I'm waiting on my PC than I can get started cooking grain and making jars. I have a beaker for pouring Agar and I have the malt and Agar Agar. I'll be using a induction burner and a thermal inferred gun to gauge temp. Should I put the contents directly into the beaker and submerge the beaker in a pot with water on the induction burner? So I don't have to transfer from a pot to a beaker than to petri?


--------------------
:rockman::rockman:
๐Ÿ„[CLICK] Grow Journal ๐Ÿ„

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Re: Ask Quick Questions, Get Quick Answers [Re: DoomShroom420]
    #28504564 -

How are you sterilizing your agar?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #28504570 -

Well once I get PC i figure that is the best route no?


--------------------
:rockman::rockman:
๐Ÿ„[CLICK] Grow Journal ๐Ÿ„

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #28504579 -

DERRAYLD said:
Plins can go straight to agar.


What is the difference between a plin and a pin?


--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." โ€“ Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

Fruiting Cubes


Golden Teachers

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #28504580 -

Proposition 122 said:
DERRAYLD said:
Plins can go straight to agar.


What is the difference between a plin and a pin?


Plin = plate pin

Regular pins grow on substrate and usually arenโ€™t sterile.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: DoomShroom420] * 1
    #28504589 -

DoomShroom420 said:
Ok, so forgetting LC altogether and will just worry about Agar. That does make things less stressful and ease the costs right now. I'm waiting on my PC than I can get started cooking grain and making jars. I have a beaker for pouring Agar and I have the malt and Agar Agar. I'll be using a induction burner and a thermal inferred gun to gauge temp. Should I put the contents directly into the beaker and submerge the beaker in a pot with water on the induction burner? So I don't have to transfer from a pot to a beaker than to petri?


Iโ€™m a little confused by the question. If you have a PC, just load the ingredients into the vessel youโ€™ll be pouring from and PC it


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Extras: Filter Print Post Top
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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   MagicBag.co Certified Organic All-In-One Grow Bags   North Spore Bulk Substrate

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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