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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28490321 -

0mar said:
Benson said:


6 varieties I've never grown before plus Golden Mammoth which I only grew once during my first grow. These are hopefully gonna turn into prints and swabs to do a big giveaway, so keep your eyes out for that in like... 3-6 months :lol:


Did you use fresh spores for these? I swabbed some plates this weekend and I didn’t see any black streaks and last time this happened they didn’t grow. So just wondering if all the spores I have are old or what gives.


They are definitely old... The Golden Mammoth is from 1/20 and the rest are some I won in a giveaway from a dope member here in 2021 that I haven't gotten around to yet. So I guess we'll see :dancer:


BeefSupremeJr said:
been getting so much shit for this in my disco i had to come post it.  laugh it up, fuzzballs, but this is the ideal media bottle setup. you may not like it but this is what peak performance looks like.






Okay thats some agar envy right there


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Benson]
    #28490331 -

right? everyone so jealous of my baba holder.  gonna pour whenever the fuck i want idc about condensation in fact ive found that condensation prevents bruising when storing plates for a long time.


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Need a lift?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Benson]
    #28490399 -

Weird I wonder why mine never leave black streaks :confused:


And the one that actually had a few black specks never grew so idk.


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“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28490446 -

The ones you see with black streaks were from swabs caked with spores. If I had swabbed that APEU plate instead of left the swab in you wouldn't see anything on the plate. And yeah sometimes they just don't come back to life. I've heard "wetter" agar (less agar in your recipe) is helpful, and I feel like I've seen teks on how to best work with old spores but I can't remember now


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Benson]
    #28490902 -

Gotcha… ya I think is pretty “wet” compared to my last recipe so we will see it’s only been like 3 days on these plates but the one with visible spores on it is like 12 days old so I might just have dead spores. No wonder I’ve only been able to grow mold from swabs.


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“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme]
    #28490927 -

BeefSupremeJr said:
been getting so much shit for this in my disco i had to come post it.  laugh it up, fuzzballs, but this is the ideal media bottle setup. you may not like it but this is what peak performance looks like.





I like it! Where can I get one?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28490944 -

:smile:

Edited by Risky Panacea (10/10/23 08:55 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Risky Panacea] * 3
    #28490948 -

Spore syringes are perfect for agar.
Now you transfer the clean growth and don't use the syringe again.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #28490954 -

Any recommendations for a source of pre-made agar dishes? continuing to make my own may cause discord in my home, so I'd like to have a backup source for when I run out and can't make fresh ones.


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Hitchhiker's Guide to the Shroomery

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Re: AGAR ENVY! (Anything and All things agar!) *DELETED* [Re: DERRAYLD]
    #28490975 -

Post deleted by Risky Panacea

Reason for deletion: :0

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Re: AGAR ENVY! (Anything and All things agar!) [Re: shakology]
    #28490981 -

First of all, I know these pictures are blurry as fuck, and I'm sorry.  I swear I'm going to have to buy a camera just for these macro shots.

Anyway, these are nats.  They were looking pretty solid for a while, but as it grew toward the edges more, the growth started to change, especially on those first two.  I don't know if it's just nats being nats or they're still dirtier than I thought.  These are T3.  Any thoughts?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Risky Panacea]
    #28491034 -

Risky Panacea said:
But is it clean? :shocked:


Looks free from visible bacterial or yeast contamination to me, mold is more difficult to say. How long after inoculation were these photos taken? And how did you inoculate them?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: covertjoy]
    #28491066 -

:smile:

Edited by Risky Panacea (10/10/23 09:31 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Risky Panacea] * 1
    #28491068 -

The syringe was squirted onto the agar liberally? The reason I ask is the cultures growing from edges of the plate are more suspect in terms of environmental contamination.

They look ok germination plates to me. I'd transfer some of the cultures until you get uniform growth and also keep the old plates for observation.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: covertjoy]
    #28491300 -

All other things being equal, if you have 4 transfer plates from the same donor plate and 1 or 2 have satellite mold colonies on the edge, is it best just to ditch those plates and transfer from the ones without satellites, or if the myc in the center looks equal to the other plates would you transfer from those as well?
The answer seems to be common sense but I would like to hear from others' experience.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Hysteria] * 1
    #28491415 -

If you must use the one with mold colonies I would do that one last


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: chikn fingaz]
    #28491480 -

Spacecadet94 said:
My DIY hobby lobby agar punch for less than 8$

PC ready or boil it in water for 5 minutes for a faster sterilization.




We got a gamer here. Nice keyboard.

I rather just run a torch for like 5 seconds and cut a triangle than spending all the other time to make a perfect circle agar wedge personally. To each their own.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet]
    #28491503 -

I cut squares. Apparently this is somehow frowned upon.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28491543 -

I'm just happy when I cut out a little wedge and don't fuck it up.

If I make a mushroom shaped punch, or...any other specific object shape -- and have a healthy culture going, will the plate grow out like the image?


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Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx] * 1
    #28491546 -

xVadisx said:
I'm just happy when I cut out a little wedge and don't fuck it up.

If I make a mushroom shaped punch, or...any other specific object shape -- and have a healthy culture going, will the plate grow out like the image?


The worst is when you cut a perfect little wedge but it won't come off the scalpel onto the new plate so you need to stab and twist the fucker until it has no choice but to enjoy it's new home. I'm sure that's terrible sterile procedure but not sure how else to approach it.



Picture tax. Are the little darker spots in the mycelium contaminants? They appear as the Myc grows out so I don't think it's little LME nuggets.

This is my last plate with food colouring too. Going clear from here on out.

Edited by Zakkery (10/03/23 10:59 PM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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