RoscoeReturns said:

killreflection said:

No pre-drying or anything, straigh into the dehydrator?

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Yes.

A dehydrator dries things. Why pre dry before drying?

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Kind a like pre boil your agar before you boil it to sterilize it

killreflection said:

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Bajazly said:

RoscoeReturns said:

killreflection said:

No pre-drying or anything, straigh into the dehydrator?

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Yes.

A dehydrator dries things. Why pre dry before drying?

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Kind a like pre boil your agar before you boil it to sterilize it

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Smellyhobbit said:

huey.bluey said:

Do any of these agar plates look salvageable? Or am I better off composting them and starting from new T0->T1 germ plate transfer. I keep seeing these molds. What are they called, how can I properly identify them. They almost look like myc but they are growing far far away from the transfer.

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4 and 5 have good growth on them, but what worries me is the persistence of these satellite contams on your other plates. It points to there being a problem with sterile procedure somewhere, and if you transfer healthy myc with bad procedure you’ll just get more contams.

You should do a thorough writeup of your agar process so we can see what’s going wrong. You shouldn’t have growth anywhere besides where you’re placing mycelium.

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huey.bluey said:

Smellyhobbit said:

huey.bluey said:

Do any of these agar plates look salvageable? Or am I better off composting them and starting from new T0->T1 germ plate transfer. I keep seeing these molds. What are they called, how can I properly identify them. They almost look like myc but they are growing far far away from the transfer.

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4 and 5 have good growth on them, but what worries me is the persistence of these satellite contams on your other plates. It points to there being a problem with sterile procedure somewhere, and if you transfer healthy myc with bad procedure you’ll just get more contams.

You should do a thorough writeup of your agar process so we can see what’s going wrong. You shouldn’t have growth anywhere besides where you’re placing mycelium.

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I put my process in the linked thread.
Ill admit sometimes I would put the lid adjacent the dish so I can hold the transfer plate in one hand to make it more stable, maybe I dropped somethings onto the plates.

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tryptkaloids said:

It's definitely possible you are dropping mold spores onto the underside of lid while you work.

I don't remove my lids all the way to avoid putting anything over the underside of the lid.

I hold the lid with my forefinger and thumb while resting the plate in the rest of my fingers, then I open my hand a little bit using gravity to open the plate.

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killreflection said:

How to do second flush with PF cakes, what's the procedure?

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tryptkaloids said:

Try stiffer agar if you're struggling with stuck transfers

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Smellyhobbit said:

killreflection said:

How to do second flush with PF cakes, what's the procedure?

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This is outlined in the PF tek post, but basically you can do one of two things. Either 1) heavily mist them until you start seeing new pins or 2) you scrape off old aborts and loose verm, dunk them for 24 hours to rehydrate, Re roll, and place back in the fruiting chamber.

I’ve done both to equal success. Personally I settled on spraying because it was way easier.

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Tiamo said:

Don't be ashamed man, that's what this thread is for.

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killreflection said:

No pre-drying or anything, straigh into the dehydrator?

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Chumbum said:

Do these grain jars look to wet? Been out of the PC for about 2 hours.

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Zav3r said:

Quick question, what's the ideal temp for fruiting?

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Zav3r said:

Quick question, what's the ideal temp for fruiting?

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Zav3r said:

Quick question, what's the ideal temp for fruiting?

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killreflection said:

Zav3r said:

Quick question, what's the ideal temp for fruiting?

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This is from bodisatta or whatever, TC, he wrote numerous guides on this site

"68-72F is a good temperature range for colonization(incubation) and fruiting. Warm temperatures encourage contaminants so room temperature is encouraged. 65-75 degrees requires no temperature adjustments for colonization or fruiting.
Cubensis mycelium grows as fast as it will from about 75-81 degrees. at above 81-83F speeds are reported to decline again. Below 65F cubensis mycelium will grow but at a slower pace until just before 32F when it will stall. Cubensis doesn't tolerate freezing but jars may be stalled in a fridge for later fruiting once they are fully colonized."

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