Chamo said:

Once you have an individual strain, it grows uniform and without sections.

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Chamo said:

but you can’t say that for sure without genetic testing

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Chamo said:

You made the claim that a monoculture is not possible, and you claimed that it there are always multiple strains present. How can you claim that without having ever done a genetic testing of the plate?

For the purposes of a hobby mycologist, obtaining a culture that provides the exact same fruiting every time is an isolate. When I have had the same culture for years, growing the exact same each time…I don’t see the point in arguing whether it is “true” or not.

Have you ever tried these things yourself or do you get discouraged by other people here?

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Chamo said:

You made the claim that a monoculture is not possible, and you claimed that it there are always multiple strains present. How can you claim that without having ever done a genetic testing of the plate?

For the purposes of a hobby mycologist, obtaining a culture that provides the exact same fruiting every time is an isolate. When I have had the same culture for years, growing the exact same each time…I don’t see the point in arguing whether it is “true” or not.

Have you ever tried these things yourself or do you get discouraged by other people here?

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Chamo said:

I think you are the one mistaken. Strain isolation is not that complicated. Once you have an individual strain, it grows uniform and without sections.

Or do you not know what a strain is?

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Sorry, but you are incorrect.

A strain can be characterized in two ways:

1. Monospore isolate: a single monokaryotic hypha germinated from an individual spore in isolation;

2. A single dikaryotic mated pair produced by two isolated parent monokaryons.

Neither of these two strain types can be produced via multispore germination. Most spores are compatible, which means that spores in close proximity will mate almost immediately via anastomosis. Bullers phenomenon will further blend and complicate the situation by the mating of dikaryons with compatible monokaryons.

The only other way to produce individual parent strains is to subject mated dikaryotic mycelium to dedikaryotization. Sectoring on plates has nothing to do with single strain isolation, and is impossible to achieve with a scalpel.

Single strain, monospore isolation is achieved via serial dilution and microscopy.



MS spore solution is diluted stepwise. That diluted solution is then spread evenly across a petri dish poured with water agar to slow growth once germination takes place. Once the monokaryotic hypha begins to grow, the cultivator use micromanipulators designed for this task to transfer the single spore isolate to a fresh petri dish, aided by a microscope.

This is known as a monospore isolate.

A breeder will then mate with either a second monospore isolate to produce a mated, fruitable dikaryotic strain or will simply allow the monospore isolate to colonize the entire dish, then use as inoculant for crossing.

This is how it's done.

Google dedikaryotization if you'd like to know how to reverse the process of dikaryotization.

Chamo said:

Very well said. If you have links to any information as to why (other than another thread on this website), I would love to know why.

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Chamo said:

It’s thought provoking but not entirely true. By the time sectors appear from MS, thousands of strains of compatible dikaryotic mycelium have consolidated. They are pure strains, composed of the collective DNA from the parings.

A little like saying Adam and Eve were the only true individual humans.

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Chamo said:

Nothing I said in my last post is even controversial. Your only evidence is “because I said so”. 

We still consider you an Individual, yet you are just a conglomeration of genetic material combined.

Sectoring plates from a T2 do not fruit consistently.

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Chamo said:

Nothing I said in my last post is even controversial. Your only evidence is “because I said so”. 

We still consider you an Individual, yet you are just a conglomeration of genetic material combined.

Sectoring plates from a T2 do not fruit consistently.

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