Stipe-n Cap said:

Rarely do they ever fruit, when they do my tubs are filled with metabolites and excessive moisture. The fruits are very few and sickly, any fruits are a rarity even when top fruited.

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Stipe-n Cap said:

I'm not sure what causes it. When I find those plates I toss them, simple as that. Those plates always originate from germ plates, never from proven pure culture or clone. There's something comingled or related to spores which is causing that morphology. I can't name it or explain it, all I know is that it produces too many problems to be qorth the effort. Heavily scalloped plates are thrown into the trash.

Not all of your pictured plates are scalloped. The mycelium is just faster in some places, this is where you select from the strongest/fastest growth to produce symmetrical growth downstream.

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thanks man

cooking some agar now, hopefully will manage to get some transfers from the first 4 before they hit the rim. If not, oh well I'll just take from a center spot.

The last 2 are pretty trash though. Sometimes I get plates like that and I wonder if it's hidden contam or just genetics. I looked at them under the scope and can't see shit.

Stipe-n Cap said:

I'd avoid these altogether, personally.



And the ones circled in red:

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Yikes. These 4 were my best ones, I sent them to LC. You can see the needle pokes.




That rhizo plate on the bottom right is literally the only good rhizo growth I've managed to get out of 4 different prints. Completely by luck too. Everything else looks like the other 3 plates that you've circled or worse.

I think I'll go back to prints and start a new round of germ plates. I'll take a lot of T1 transfers and cull them mercilessly. Might actually get a use of that box of 500 60mm petris.


I'm hoping I'll get rhizomorphs but I probably will not, so how do I pick good tomentose? Those 3 you circled in red are the best I've gotten so far, so if they are not good enough, I need to know what to aim for. (except for that GT, it looks fucky even to me)

Many thanks for taking the time to point out which ones you'd discard, appreciate it even if it hurts

NFLProof said:

When doing many transfers at once what's the most efficient and trackable method of labeling? I've been making all my transfers then labeling once the session is done outside the sab. @SirP do preprint your labels? That seems like it would be a logical way to do it.

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Yahra said:

I buried a PF cake in coir 2 days ago.

The cake is completely covered with coir. Maybe 0.5 inch thick layer.

Should i mist the coir if it's dry or wait with the misting until i see mycelium?

Coir was at field capacity when i buried the cake.



I poked a bunch of holes with a needle into the bag after i took that photo.

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Probably good to go. I never misted this one.

GT - 9 intact 4oz brf cakes - cased with coir - 19.6.23