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Re: Ask Quick Questions, Get Quick Answers [Re: SeaSalt] * 3
    #28320667 -

SeaSalt said:
Yahra said:
You don't need verm in this hobby.

Bulk? Just plain coir
PF tek? Replace verm with coir

Coir is all you want.


You put coir in your PF instead of verm? Why?


Because i don't like verm. The smell, the dust, all of it.

And since you can make coir pf cakes and roll them in it, i want to get rid of verm completely.


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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 3
    #28320670 -

I've seen this method, just be careful with your jars and make sure they don't get too compact.


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Meditation Principles

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IRC: ##mycology on Libera.chat come hang!

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Re: Ask Quick Questions, Get Quick Answers [Re: Newshroom42] * 1
    #28320678 -

Newshroom42 said:
Hey guys,

When do you think I should send these jars to bulk?

I inoculated them with agar 2 weeks ago and just did a second shake yesterday as they were around 70%-90% colonized. It’s been about 1.5 days from the second shake and everything is covered with mycelium. It’s not as thick as it was before the shake so Im thinking it should be ready in like a day or two.

I have one grow under my belt but it was definitely bacterial as I went MSS to grain bags. It had a completely different texture like melted ice cream and was a pain to break apart. First bag contamed before the first flush, but second bag is still going strong one month later. It is lacking with yield, but no contam yet.

I’m assuming it should look something like my first picture, but all over the jar.

Any advice would be most appreciated. Thank you!

This was the most colonized jar before the second shake:


This is one of the jars about 36 hours after the shake:



The others are more negative. But I think it's healthy growth on rye berries that were prepped too dry.


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How I grow mushrooms πŸ„
How I do agar 🦠

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Re: Ask Quick Questions, Get Quick Answers [Re: Newshroom42] * 3
    #28320691 -

Newshroom42 said:
Also I shook the jars at around 10-15% and it grew out to 70-90% in about 5 days

I guess worst case is send it and see what happens if it looks good. My last grow survived going to bulk and it was hella bacterial. I’m doing shoeboxes so there are more chances of getting some fruits

In terms of what you are seeing in the jars, are you seeing the issue only in the shaken jar or both of them?


Shaken jar looks more concerning, but honestly the more I look at it the more I suspect it’s healthy. The myc just doesn’t look as thick as it should be, but I’ve had jars with thin growth do just fine.
It’s not ideal, but you could hedge your bets and go for a lowish spawn:sub ratio. Maybe 1:2. If I suspect a jar is bacterial, it gets top-fruit or 1:1 in box.
Give it a few days and see if it fills out more; otherwise I don’t really see anything wrong with sending both those jars.


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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head]
    #28320699 -

Anyone know what would cause a jar of WBS to have a clump in otherwise well prepared grain? Everything else rolls freely and I’d say 5 of the 6 jars are perfect. Then I’ll get a big ole clump in one.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Way] * 2
    #28320701 -

stockw said:
Should casing layers of coir generally be looking moist?

Mine are pretty fkn dry looking on my shoeboxes day 7.. Colonisation is going ok though, maybe slowed down a bit the past couple days.

My substrate mix was 1:3 which I know seems dry but that was field capacity for my coir.



If it's just straight coir, the top layer can get pretty dry in shoeboxes. One of the reasons I prefer coir and verm. Should be fine when it colonizes and moisture draws up through the myc.

I'd say a light misting might would be okay if it's really stressing ya. Emphasis on light.

1g coir to 3g water does seem waaaaay too dry though. Maybe you have weird coir. I'd bet more on you're definition of field capacity is slightly off though.


Yeah I've done half of them with verm half without because as I got to the bottom of my mix the water had sunk & it was way too wet so dialled it in with verm.

I've just had a peek & they don't seem as dry as they did earlier so I'll leave em be for now but will keep an eye out.

How dare you question my definition of field capacity, I watched a 320p video of someone squeezing a ball of coir until 7 drips came out. I'm completely in the know. :allrightythen:

Cheers for the advice wayman! Much appreciated.

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #28320711 -

Smellyhobbit said:
Anyone know what would cause a jar of WBS to have a clump in otherwise well prepared grain? Everything else rolls freely and I’d say 5 of the 6 jars are perfect. Then I’ll get a big ole clump in one.


I’ve had this happen plenty of times. Clump(s) in seemingly axenic or otherwise perfect spawn. Only thing I can think is negligible bacterial presence that is either dormant or wasn’t strong enough to survive. (unlikely) or some sort of weird cluster of grains that got arranged in such a way. (reaching)
Those are my two β€œhypotheses”
Otherwise I’m stumped.


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----<><><>----

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⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 2
    #28320736 -

Yahra said:
You don't need verm in this hobby.

Bulk? Just plain coir
PF tek? Replace verm with coir

Coir is all you want.


Seems like it's a bit early for you to be recommending this before you've had any successful PF coir grows yourself :smile:

Yahra said:
How to prepare coir as a substitute for vermiculite in PF tek?

Just take a brick, tear it apart, put it in a bucket and pour boiling water onto it?

How long let it steep?

Just use any amount of water and squeeze it until it has reached fiel capacity or do i need to use the correct amount of water from the start?

What else to consider?

Sorry if this has been asked before but i fell for outdated advice multiple times and i don't dare to use the board search anymore.


Water doesn't need to be boiling, since you're going to sterilize it later anyways, it just makes the process longer. Warm is good since it makes the coir absorb water faster. Do try to get coir without any trich added, for obvious reasons. Not sure how common that is, but it is a thing.

I bring it up to field capacity, add the BRF, then add more water and squeeze again, since the BRF absorbs a bit of water. But PF coir cakes aren't common so there isn't as much consensus on what to do.

I do 2:1 hydrated coir to BRF by weight, but I'm not convinced that's the best ratio, I need to play around with it more. I suspect that's less BRF than in Mateja's thread that you linked. Looking for a bulletproof tek here is a mistake since it's not a common thing, so you're going to have to accept some experimentation yourself.


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Hermit's index of useful posts, books, and links

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Re: Ask Quick Questions, Get Quick Answers [Re: hermitic] * 2
    #28320741 -

Thanks, very useful. :thumbup:

hermitic said:
Seems like it's a bit early for you to be recommending this before you've had any successful PF coir grows yourself :smile:



TouchΓ© :cool:


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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head] * 1
    #28320743 -

fiddle_head said:
Smellyhobbit said:
Anyone know what would cause a jar of WBS to have a clump in otherwise well prepared grain? Everything else rolls freely and I’d say 5 of the 6 jars are perfect. Then I’ll get a big ole clump in one.


I’ve had this happen plenty of times. Clump(s) in seemingly axenic or otherwise perfect spawn. Only thing I can think is negligible bacterial presence that is either dormant or wasn’t strong enough to survive. (unlikely) or some sort of weird cluster of grains that got arranged in such a way. (reaching)
Those are my two β€œhypotheses”
Otherwise I’m stumped.


This happens right out of the PC. After a little rest. Hopefully it’s not bacteria πŸ˜‚


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 2
    #28320768 -

If you had these two plates to take transfers from where would you take from? Both plates are clones of one fruit.






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Re: Ask Quick Questions, Get Quick Answers [Re: mycomaxx] * 2
    #28320770 -

I wouldn’t transfer from a clean clone plate at all.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: mycomaxx]
    #28320773 -

12 o'clock.


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Re: Ask Quick Questions, Get Quick Answers [Re: WhatEyeFind]
    #28320775 -

WhatEyeFind said:
Quick question

Can I use stale wheat berries? About a few years old from a prepper who has about twenty 5 gallon pails full.


If they don't have any obvious issues then they will be fine. Un-milled grains keep very well if the container is sealed.

I brew beer and have used old grains more than once... I could not tell the difference. (Malted barley, you can also chew to see if it still tastes good... I wouldn't try that with wheat, you'll crack a tooth!)


--------------------
We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley

Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo

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Re: Ask Quick Questions, Get Quick Answers [Re: Yuggoth]
    #28320789 -

Since my petris are multiplying like rabbits, I got thinking I should try making LC and use it to inoculate 100lbs of premium organic grain.

I'm kidding, I'm just going to make like 100ml for funsies, see what happens. Worst case I lose a pint or quart of grain.

My question is how to properly aspirate a sample of the LC to put it to agar for testing? Isn't my hand holding the syringe going to be directly over the LC bottle opening, potentially dropping contams (in a SAB) in the LC? Do you tilt the bottle at 45degrees to avoid that?

For inoculation, I guess it's a bit easier since I can just drop an agar wedge and with the long scalpel handle my hand is not going to go over the mouth of the LC bottle.


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra]
    #28320791 -

Yahra said:
SeaSalt said:
Yahra said:
You don't need verm in this hobby.

Bulk? Just plain coir
PF tek? Replace verm with coir

Coir is all you want.


You put coir in your PF instead of verm? Why?


Because i don't like verm. The smell, the dust, all of it.

And since you can make coir pf cakes and roll them in it, i want to get rid of verm completely.


I can see you're trying to be helpful but you've never even hydrated coir yourself.
PF tek has held the test of time and advising noobs to deviate from it isn't helpful. (At least provide a link to the Tek if you feel you must respond.) Judging FC takes a little practice and Majeta is an experienced cultivator. Noobs tend to deviate from Teks and make things more complicated in the first place. Encouraging it just lessens their chance for success.

Most people don't have such a visceral negative reaction to vermiculite. Medium verm provides small air pockets in cakes, helps prevent over compaction, and aids colonization.

I cased my first cakes in coir and grew them in ziplock bags. I'd never be so presumptuous as to dispense advise, my very first grow, using a tek I hadn't completed. I've only been growing a year and I wouldn't do it today either. I'm still a noob.

Coir get's expensive when you're running 20 shoeboxes or more at 1:4 to 1:6. 50/50 coir/verm helps my subs retain moisture and provides better surface conditions in my environment. Is verm necessary? No. Does it save me money? Hell yes. It seems unlikely that you'll understand the value of verm because you flatly refuse to use it and you have no experience to draw from. Telling newcomers verm is a waste of money isn't the case for everyone or every situation.


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Re: Ask Quick Questions, Get Quick Answers [Re: SeaSalt]
    #28320803 -

Yeah, i still don't like verm.


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Re: Ask Quick Questions, Get Quick Answers [Re: altford78]
    #28320806 -

altford78 said:
My question is how to properly aspirate a sample of the LC to put it to agar for testing?


Everything has a risk. You either have to open the jar and incur that risk, or use an injection port and incur that risk.

I am not sure what is better, but when I grew and tested good LC, I did it this way, with a SAB...

* To inoculate the LC, I opened the jar -- but I was able to add inoculum without passing my hands over the jar.

* To withdraw LC for testing, I used the injection port -- this was the first time the port had been used post-sterilization.

I still have nice looking jars and test plates.

My next question is much like yours... How to withdraw verified clean LC for use. Dare I trust the port a second time? Or do I open the jar?

I think I am going to open the jar. I have some 4" stainless, reusable, sterile dispensing needles. That will let me suck up the LC with the jar at an angle, and no hands over it. I can then easily dispense right into grain jars. If I want to inoculate bags, I'll swap the blunt dispensing needle for a new sterile 16 ga needle.... and hope it does not clog. My LC is chonky.


--------------------
We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley

Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo

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Re: Ask Quick Questions, Get Quick Answers [Re: Yuggoth] * 2
    #28320809 -

Yuggoth said:
altford78 said:
My question is how to properly aspirate a sample of the LC to put it to agar for testing?


Everything has a risk. You either have to open the jar and incur that risk, or use an injection port and incur that risk.

I am not sure what is better, but when I grew and tested good LC, I did it this way, with a SAB...

* To inoculate the LC, I opened the jar -- but I was able to add inoculum without passing my hands over the jar.

* To withdraw LC for testing, I used the injection port -- this was the first time the port had been used post-sterilization.

I still have nice looking jars and test plates.

My next question is much like yours... How to withdraw verified clean LC for use. Dare I trust the port a second time? Or do I open the jar?

I think I am going to open the jar. I have some 4" stainless, reusable, sterile dispensing needles. That will let me suck up the LC with the jar at an angle, and no hands over it. I can then easily dispense right into grain jars. If I want to inoculate bags, I'll swap the blunt dispensing needle for a new sterile 16 ga needle.... and hope it does not clog. My LC is chonky.


You know what's better?

G2G

:rollsafe:


--------------------
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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho]
    #28320818 -

G2G is absolutely on my to-do list. :smile: I love the idea of maintaining a more contamination-resistant culture.

I do want to learn to do LC well too, just because. It's all fun ... even if it turns green.


--------------------
We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley

Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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