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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: CarRacer]
    #28005511 -

CarRacer said:
Getting my start with agar. Excited to get some clean plates and in turn spawn.




Now we're talking. Don't hesitate to ask questions here if you have any.

PS: you SAB holes look small to me. Don't be afraid to make 6" holes - it's actually better than smaller ones.


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096]
    #28005534 -

You're not wrong, they're 4.5". It's sufficient for now, I can always make 2.0 after I use it more and understand what I need better.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CarRacer]
    #28005563 -

The larger the hole, the less air that is moved (bellows) as you take your arms in and out of the box.


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πŸ…ƒπŸ„΄πŸ„°πŸ„Ό  πŸ„²πŸ„»πŸ„ΈπŸ„½πŸ„ΆπŸ…†πŸ…πŸ„°πŸ„Ώ

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas] * 1
    #28005567 -

These are T1's of original ms plates.  I have no idea what I am actually growing. 

Some of the myc/growth seems to be defined and some looks almost like a smooth cloud.  There appears to be myc, but there is a fuzzy layer on top of the myc.  Is this mold/contam? 

The PE strains seem to have the most amount of fuzzn and height.  Can I take transfers from any of these or am I better placed going to water agar to see if I can get cleaner myc?



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πŸ…ƒπŸ„΄πŸ„°πŸ„Ό  πŸ„²πŸ„»πŸ„ΈπŸ„½πŸ„ΆπŸ…†πŸ…πŸ„°πŸ„Ώ

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas] * 1
    #28005573 -

Bobgas said:
These are T1's of original ms plates.  I have no idea what I am actually growing. 

Some of the myc/growth seems to be defined and some looks almost like a smooth cloud.  There appears to be myc, but there is a fuzzy layer on top of the myc.  Is this mold/contam? 

The PE strains seem to have the most amount of fuzzn and height.  Can I take transfers from any of these or am I better placed going to water agar to see if I can get cleaner myc?





Looks good to me but I'm far from an expert here.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bajazly]
    #28005653 -

Smellyhobbit said:
Express this

:shitty:


Do I see a ryno tek in our future?


--------------------
Initial Grow log
My First PF TEK
DIY Brown Rice Flour
Remove Condensation from active plates
My first Agar Pour

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas] * 1
    #28005657 -

Bobgas said:
These are T1's of original ms plates.  I have no idea what I am actually growing. 

Some of the myc/growth seems to be defined and some looks almost like a smooth cloud.  There appears to be myc, but there is a fuzzy layer on top of the myc.  Is this mold/contam? 

The PE strains seem to have the most amount of fuzzn and height.  Can I take transfers from any of these or am I better placed going to water agar to see if I can get cleaner myc?





Those all look pretty good from the pictures


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Re: AGAR ENVY! (Anything and All things agar!) [Re: schpat] * 1
    #28005695 -



Psilocybe cyanescens

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CarRacer] * 1
    #28006331 -

Also, the carpet is not doing you any favors.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 4
    #28006772 -

Big thanks to the member (I don't know if I should name you but you know who you are friend) who sent me those PEU q-tip swabs. You are the q-tips master brother.

Only 4 days after streaking plate:


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28006828 -

Any tips on swab streaking? Got myself a bacterial bonanza going on over here.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 2
    #28006852 -



part1 is also interesting


--------------------
β€œAll this buttoning and unbuttoning”

Edited by 999999999 (10/19/22 06:25 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 2
    #28006861 -

Smellyhobbit said:
Any tips on swab streaking? Got myself a bacterial bonanza going on over here.


Yeah, I do have one: get a better swab donor :lol:
(Seriously I did nothing fancy: I just "Z" streak the plate with the Q-tip. All the credits go to the person who sent it to me. Clean AF Q-tip Tek bb!)


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #28007600 -

Smellyhobbit said:
Any tips on swab streaking? Got myself a bacterial bonanza going on over here.


I know this is the agar thread, but if you have a really bacterial swab that you need to salvage, Josex puck poke tek has an answer for swabs that works really well.
Josex' Poke: No Mercy for Bacteria


From a swab in 5 days ready for transfer to agar.

These are some plates that came from the same swab.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc] * 2
    #28007605 -

ReverendMyc said:
Smellyhobbit said:
Any tips on swab streaking? Got myself a bacterial bonanza going on over here.


I know this is the agar thread, but if you have a really bacterial swab that you need to salvage, Josex puck poke tek has an answer for swabs that works really well.
Josex' Poke: No Mercy for Bacteria


From a swab in 5 days ready for transfer to agar.

These are some plates that came from the same swab.



Made 12 of them already πŸ’ͺ


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096]
    #28007913 -

First grow! Straight grain inoculation didn't make sense, since it seems easy to contaminate and I was going to be working with a spore print, so wanted least waste as possible.

2022-10-11: dropped spores into 4 LMEA dishes
2022-10-13: got anxious reading the forum thinking that I didn't "smear" the spores, so I dropped into one additional LMEA dish and scratched the media up to help
2022-10-17: got anxious again seeing the lack of any white mycelium, cooked up some dextrose+starch+yeast agar and poured and inoculated 3 more dishes. Up to 8 now

2022-10-20: actually there was some growth, just I didn't see it, since it was mostly translucent! I got it on good authority though, that it is fine and even transfers can be done on it, so I did some.





The wedges seem pretty small, but I had little growth to work with, it was difficult to see due to translucency, but I will see if it works out and if not - there are more plates :laugh:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 2
    #28007917 -

Panaeolus clones of Bunnell and Nec-D on 1% LME agar.




and a semilanceata MS culture




--------------------
πŸ„PANAEOLUS IN MONOTUBSπŸ„
πŸ„OFFICIAL SEMILANCEATA THREADπŸ„



⭐Baba's Write Ups and Link Collection⭐

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Re: AGAR ENVY! (Anything and All things agar!) [Re: worynox]
    #28007924 -

worynox said:
First grow! Straight grain inoculation didn't make sense, since it seems easy to contaminate


This might be the greatest thing ever said by a beginner ever...+5 shrooms

Small transfers are good, less chance for tag along contams.


--------------------

Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #28007928 -

rumfor69 said:
worynox said:
First grow! Straight grain inoculation didn't make sense, since it seems easy to contaminate


This might be the greatest thing ever said by a beginner ever...+5 shrooms

Small transfers are good, less chance for tag along contams.


gay grain inoculation only


--------------------
Confused? Well now you can!
HHG - cheapest way to start - how i roll

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28007935 -

That’s bisexual inoculation erasure.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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