sandman420 said:

I think the first one is a slime mold. A food coloring reaction should only happen on older parts of plates not so soon. Or is it already grown out with weak mycelium it's hard to see. Maybe just bacteria.

The second one looks like it's growing inside the agar instead of on top. Probably a competitor mold.

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Both sets were done on 2-15 so almost three weeks ago. Both were using the same agar plates.  Maybe I should get/make agar plates with a more neutral color to take some of the variables away.
I used the same tools for both and between each transfer, sanitized the tools.
If there was a slime mold issue, it only happened with the same strain unless the original pieces had the issue, but that agar didn't appear to show it.
Maybe someday I'll figure it all out.

bonanzaman said:

Comments welcome about agar.
These are second plates from a clone and curious about what I'm seeing.

The first two are from two of the same strain. I'm thinking the green is the myc sucking up the food coloring of the agar. The edges, to me,  look good.



The second two are also clones from a different strain.


Any Thoughts?

Thanks
BM

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Hard to tell but you're probably right, probably sucking up food coloring, since the edges don't have it.

I'd skip food coloring at first, at least green, since it will make it hard to see if it's got contam. At first glance I actually thought this was algae. haha.

If you have to use colors, a tip: don't try red, it's known to be anti-fungal. But I recommend to try black. It's charcoal, so it's also a nutrient.  I know rhizo isn't needed, but I've had nothing since rhizo since I started using it. Rhizo looks cool, but it's also easier to tell from common molds, which look more like fluffy mycelium. Rhizo on black agar:

Prakriti said:

My first agar pin 😁

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bigfootscreepyuncl said:

Nice! pluck that off the plate and drop it onto a new one, that's all there is to it! Plate pins usually put off really strong growth to so you'll have an easy time cleaning it up.

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My favorite shrooms so far are some x7x plin (plate pin) clones. I grew one shoe box, ate some, had the best trip of my life, and am now growing 3 more boxes.
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CONTAM QUESTION:
So the pic below is a 10-day old T1 clone off a stipe clone culture. I had to turn the contrast way up to show what I'm asking about: The fuzz on the left and top of the grow. (The contrast also brought out scratches and tape residue on the top of the plate, ignore that.)

There is what looks like very fine mycelium growing off the left and top. In real life, it's hard to see unless you look closely from certain angles.

Is this likely contam, or likely mycelium?
thank you.

contam question

The Fresh Prints said:

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nektar61 said:

bigfootscreepyuncl said:

Nice! pluck that off the plate and drop it onto a new one, that's all there is to it! Plate pins usually put off really strong growth to so you'll have an easy time cleaning it up.

---

My favorite shrooms so far are some x7x plin (plate pin) clones. I grew one shoe box, ate some, had the best trip of my life, and am now growing 3 more boxes.
----
CONTAM QUESTION:
So the pic below is a 10-day old T1 clone off a stipe clone culture. I had to turn the contrast way up to show what I'm asking about: The fuzz on the left and top of the grow. (The contrast also brought out scratches and tape residue on the top of the plate, ignore that.)

There is what looks like very fine mycelium growing off the left and top. Is this likely contam, or likely mycelium?
thank you.

contam question

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Likely mycelium, proceed. Worst-case, you’ll know for sure in a day or two.

bigfootscreepyuncl said:

Prakriti said:

My first agar pin 😁

---

Nice! pluck that off the plate and drop it onto a new one, that's all there is to it! Plate pins usually put off really strong growth to so you'll have an easy time cleaning it up.

---

Boozie said:

nektar61 said:

bigfootscreepyuncl said:

Nice! pluck that off the plate and drop it onto a new one, that's all there is to it! Plate pins usually put off really strong growth to so you'll have an easy time cleaning it up.

---

My favorite shrooms so far are some x7x plin (plate pin) clones. I grew one shoe box, ate some, had the best trip of my life, and am now growing 3 more boxes.
----
CONTAM QUESTION:
So the pic below is a 10-day old T1 clone off a stipe clone culture. I had to turn the contrast way up to show what I'm asking about: The fuzz on the left and top of the grow. (The contrast also brought out scratches and tape residue on the top of the plate, ignore that.)

There is what looks like very fine mycelium growing off the left and top. Is this likely contam, or likely mycelium?
thank you.

contam question

---

Likely mycelium, proceed. Worst-case, you’ll know for sure in a day or two.

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shroomyg123 said:

Are these APE t2's ready for grain? or should i take another transfer? I used liquid food coloring black dye, it seems to be turning slightly organ, the mycelium seems very white and strong but not in the center. Is it ready or ?

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Personally, I'd transfer one more time. No particular reason other than just being thorough. But why not do both? Prep some grain, prep some plates, take some slices from your favorite plates and transfer, cut some big ol' wedges and throw them to the grain. Potential win-win.

nektar61 said:

bonanzaman said:

Comments welcome about agar

Any Thoughts?

Thanks
BM

---

Hard to tell but you're probably right, probably sucking up food coloring, since the edges don't have it.

I'd skip food coloring at first, at least green, since it will make it hard to see if it's got contam. At first glance I actually thought this was algae. haha.

If you have to use colors, a tip: don't try red, it's known to be anti-fungal. But I recommend to try black. It's charcoal, so it's also a nutrient.  I know rhizo isn't needed, but I've had nothing since rhizo since I started using it. Rhizo looks cool, but it's also easier to tell from common molds, which look more like fluffy mycelium. Rhizo on black agar:

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