p9hu7 said:

As the dikaryotic hyphae lengthens it forms clamp connections between septa to transfer nuclei. Is this what you're getting at? Or do you mean fusion of the nuclei?

"The dikaryotic phase is technically not diploid, since the nuclei remain unfused until shortly before spore production.) "

"Each basidiospore germinates and generates monokaryotic haploid hyphae. The mycelium that results is called a primary mycelium. Mycelia of different mating strains can combine and produce a secondary mycelium that contains haploid nuclei of two different mating strains. This is the dominant dikaryotic stage of the basidiomycete life cycle. Thus, each cell in this mycelium has two haploid nuclei, which will not fuse until formation of the basidium. Eventually, the secondary mycelium generates a basidiocarp, a fruiting body that protrudes from the ground"

https://opentextbc.ca/biology2eopenstax/chapter/classifications-of-fungi/

This should probably be its own thread.

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multifractal said:

I was more talking about the sharing of genetic material as seen in karyogamy between two monokaryotes not the fusion of individual haploids which only occurs when spores are formed. I see people talk about "dikaryotes fusing" and was wondering what exactly this meant. I believe septa only allow material interchange between cells of the same dikaryotic hyphal strand.

But yeah, if we're branching into new thread territory that's fine.

EDIT: just saw your new thread link

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p9hu7 said:

Trying to select for a true single strain isolate with a scalpel is like trying to pick up a pencil if your fingers were the size of sky scrapers.

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Exactly.

Here are some up close shots of cube mycelium. Seperating out a single thread and knowing you had done so would be pretty much impossible .



The first two pictures are a "clone" (tissue transfer from a fruit). Even the ones in these shots that look like individual strands could be 10-12 or 100 running together. They are not bound by limitations of relativity when it comes to cellular sizing lol. They are very plastic creatures and very aware.

The best methods I have seen involve a lot of calculated spore dilution (I'm sure there are better tech terms for this) and a lot of staring through a microscope and counting spores on a grid. It is totally unnecessary IME in order to get stellar results with cubes.

Great practice for working with cordyceps though. They are weird critters.

p9hu7 said:

10^fuckload

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Psilocyberat said:

Just got my first succesful agar dishes. Such a good feeling!

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Yeah baby! It's all uphill from here

Lovage said:

I dont see anything wrong with those plates?? where do you see this so i can get educated please?

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joze said:

Psilocyberat said:

Just got my first succesful agar dishes. Such a good feeling!

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Yeah baby! It's all uphill from here

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