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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1]
    #27353917 -

You don't need to heat the agar at all.

At home, I boil for 3 minutes on the stove. But in the lab, I put it on the stirring plate for an hour with no heat.

If I had a stirring plate at home, I'd definitely be doing it this way


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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #27354396 -

Doob, i hit boil with my agar when i prep it to hydrate it thoroughly, idk would the stir plate boil it no problem? Agar boils at like 200F or something

i was lucky to get a little PEU cluster to grab a clone from before i stopped fruiting completely a while ago, and a month after putting it on agar it threw invitro pins. This is the first pin to pop up.



I'm excited to have something to fruit while i get my agar right, cause the empty fruiting room makes me sad.  What would you do if you were me now? My goal is to save some good clone genetics from these to fruit through this year and maybe next. Gives me ample time to find new genetics for myself to grow and practice agar more.  I'm not after a single monoculture jsut yet, i just need some moderate consistency.

Should i take transfers out of the sectors on this clone to fruit and grab clones from? Or should i just throw this straight to grain and grab a few of the top clones to go back to agar and fruit again. Would it be best to keep going or could i save those genetics long term after the second generation? (or third since this is an invitro pin right?)

What would you do with my goal in mind for these PEU?

Edited by moshe1 (06/18/21 11:31 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1] * 2
    #27354487 -

Love this TAT culture


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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1]
    #27354488 -

moshe1 said:
High Temps: Check

Mixing the Medium: Check

I think you should try and let us know- i think you're on a search for why it wouldn't work rather than why it should :smile:

but i think it might just not heat fast and intensely enough to get it thoroughly melted fast- might need some extra time?


Makes sense moshe1. Ain't got the patience for the slow boat.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1]
    #27354523 -

moshe1 said:
Doob, i hit boil with my agar when i prep it to hydrate it thoroughly, idk would the stir plate boil it no problem? Agar boils at like 200F or something


found on ddg search askinglot.com:
At what temperature does Agar liquify? Agar exhibits hysteresis, melting at 85 °C(358 K, 185 °F) and solidifying from 32-40 °C(305-313 K, 90-104 °F). This property lends a suitable balance between easy melting and good gel stability at relatively high temperatures.

It really depends on the efficacy of the hotplate. I haven't even unboxed mine. Deciding whether or not to keep it, though actually what follows convinced myself to keep it

I fucking hate standing at the microwave, boilguarding the media bottles. Even if I just do a liter at a time and then pour it into media bottles for the PC, I love me some set it and forget it. Currently, I nuke it out of the fridge then set it in a water bath on the worktable and come back later, check the water temp which will be the agar temp, and pour.

Like I'm gonna do, seeing the hour, in the morning.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27354820 -

Trying out yeast for the first time to try and get me away from slow growth and more toward rhizo.

Is this a good / tried & tested recipe?

10g agar agar
8g LME
500ml distilled water
1g yeast

?

Edited by King87 (06/19/21 07:59 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: King87]
    #27355022 -

King87 said:
Trying out yeast for the first time to try and get me away from slow growth and more toward rhizo.

Is this a good / tried & tested recipe?

10g agar agar
8g LME
500ml distilled water
1g yeast

?


:thumbsup:


--------------------

Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: King87]
    #27355057 -

King87 said:
Trying out yeast for the first time to try and get me away from slow growth and more toward rhizo.

Is this a good / tried & tested recipe?

10g agar agar
8g LME
500ml distilled water
1g yeast

?


I would point out that rhzio doesn't always equate to a good culture... but they sure are pretty! :cool:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27355266 -

True


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1]
    #27355331 -

moshe1 said:
Doob, i hit boil with my agar when i prep it to hydrate it thoroughly, idk would the stir plate boil it no problem? Agar boils at like 200F or something



Yeah but won't these temps be achieved during sterilization?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #27355374 -

That’s what I thought too but boiling it before the pc does make a difference ime, they for sure come out clearer. I microwave it a couple minutes.


It doesn’t really make sense unless there’s some new protein or something formed when it’s heated and cooled down the first time.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #27356475 -

That's interesting. When I pour plates this way, they're crystal clear. But, this is a different recipe than we typically use for myco. It's an agar, yeast, tryptone, NaCl mixture.

I suppose it's the lme sugar that doesn't fully solubilize without boiling at the high concentrations we use it.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #27356530 -

I use cold tap and lme comes out clear enough for me.

Give it a shake n PC. No fuss.

When I want it clear as glass ill stash the broth in the fridge for a few hours b4 pcing.


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

Edited by mushboy (06/20/21 03:44 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #27356551 -

A.k.a said:
That’s what I thought too but boiling it before the pc does make a difference ime, they for sure come out clearer. I microwave it a couple minutes.


It doesn’t really make sense unless there’s some new protein or something formed when it’s heated and cooled down the first time.


https://www.shroomery.org/forums/showflat.php/Number/27354523

My new hot plate/magnetic stirrer g0es to 350 C / 667 F…

soon as I can I’ll try it
for science


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27356621 -

Seeing strong mycelium on this T2 Melmak plate.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: mandelbro]
    #27357377 -

Undecided regarding this TAT germination and transfers.

11

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27357420 -

I... ummmm


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #27357427 -

I'm not liking the satellite ring or the look of the mycelium but I can't decide if it's contamination at this stage.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #27357432 -

LadysKnight said:
I... ummmm


I feel like I know the rest of this

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27357433 -

There are a lot of opinions and experiences with those rings/halos, but none of mine are good.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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