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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27351877 -

:firecum:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27351888 -

Extra protein? Cypress hill knew best..... high enriched.

Sloppy like an old jalopy.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27351964 -

Crackatoa tat germinated cleanly, ready for a transfer later.

PR lc tested clean and healthy, off to grain it goes.


Edited by DERRAYLD (06/17/21 10:58 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27352030 -

Got any surface-lit pics of the tat?


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #27352104 -


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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27352140 -

Hey guys just wondering if i should have transfered sooner or is now ok? I feel maybe I should have tranfsered already but when its any smaller I have trouble identifying growth. Im thinking I should transfer from around 4-5 oclock where the growth on the edge seems most even? This is at T2 plate, T0 was mss. Any help is greatly appreciated.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27352148 -

DERRAYLD said:




Thanks D, I'd like to see how those transfers progress.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #27352151 -

I'm transferring to a couple plates this evening, will post updates.

This was 4 days ago, for reference.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: milkboy]
    #27352191 -

Transferring now is fine, not a big deal.


--------------------
:chems:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lenz]
    #27352197 -

Yeh it's been ready for 2 days, just wanted a bit more surface area to take a couple transfers.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27352547 -

Some random plates
There was a tiny satellite contam on the natalensis plate. Should be fine now


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Regis] * 3
    #27352863 -



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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Fresh Prints]
    #27352943 -

I get this odd honeycomb like contam on some of my plates. Usually it's after they sit for a bit but not in this case, has this been talked about here before?





Also, this PE plate was very rhizo and appeared clean, but after sitting for a little while appears to have developed some sort of pin mold perhaps?

This is also something i've been seeing here and there in addition to the honeycomb like growth just mentioned.








Perhaps just aerial mycelium? Residual tomentose growth after the plate is fully colonized?

No other agar contams ever present on my plates besides those two.

Input greatly appreciated, character assassination also welcome.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Fresh Prints]
    #27352971 -

The Fresh Prints said:




:homerdrool:


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear


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Re: AGAR ENVY! (Anything and All things agar!) [Re: PitcherCrab]
    #27353054 -

this was happening on a lot of my plates and it was confusing as fuck, couldnt figure out what it was



until recently this contam plate caught some foreign white mold and a very sneaky one.  its a lot more puffy/aerial than it looks in this picture here. when it just starts growing it looks just like myc, and has a thin halo around the growth just like cube myc does. never changes color, so you could have some fuzzy white on a plate somewhere and never catch it



i hate white molds

Edited by moshe1 (06/18/21 04:37 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr. Mushie]
    #27353291 -

Any chance it's just pinning? How long for it to show up and have you ever let it keep going to see what happens?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVandalx]
    #27353711 -

Looking for some help.

A bunch of my germination plates don't look right. There's growth on the streak line but it always ends up suspicious and transfers do also.

Can it be as simple as changing my agar recipe?



I'm using 10g agar 10g lme 450 ml water.
I think my sterile technique is ok.

Spores are from a reliable vendor
Thank you!


--------------------
.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Atomsplit]
    #27353751 -

whats your agar recipe? doesnt look too crazy there just keep transferring. I've had some wonky looking germ plates and the T1's and T2's were also throwing weird growths, few transfers later i have clean looking rhizo growth on my lowest nutrition plates.

try 5G LME and 10G AGAR for 400ml of water, and use those for your T2 and T3's. You could add a gram of Yeast in there too.


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Edited by moshe1 (06/18/21 04:39 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1]
    #27353783 -

Here's an agar question for y'all.

I came across a hot-plate magnetic stirrer. I know peeps use them for LC. Can I melt and combine my agar ingredients byn heating to like 185F with the stirrer going? It'll be fun getting the stir bar out in Arctivated Charcoal LMEA. I got a tool. :rofl:


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27353792 -

High Temps: Check

Mixing the Medium: Check

I think you should try and let us know- i think you're on a search for why it wouldn't work rather than why it should :smile:

but i think it might just not heat fast and intensely enough to get it thoroughly melted fast- might need some extra time?


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Edited by moshe1 (06/18/21 05:11 PM)

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