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Re: unknown pans [Re: Zifozonke]
    #26834218 -

Good to hear. I haven't been keeping up in the pan thread for a bit. I'll definitely have to check it more to see Jake's pics.. that'll be amazing I'm sure!
I do have a nice looking LC of these that is ready to use as soon as I can find some manure around here.


--------------------
LAGM 2021
LAGM 2020
Trade List

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Re: unknown pans [Re: One of Us]
    #26853454 -

Quote:
Hanswermhat recently got a tray with a few fruits and then did them outdoors and got some lovely fruits...



Do you have a link to that thread?

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Re: unknown pans [Re: Zifozonke]
    #26853656 -

Zifozonke said:
Jake mentioned in the pan thread that he has a tray(s) getting ready to fruit..thatll be interesting 4 sure-he seems excited...so am pretty sure once everythings been checked out and tests done-we will get some news.



I think his trays stalled out.

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Re: unknown pans [Re: Alan Rockefeller] * 4
    #27026717 -

Well it's been almost a year...wow
This year has flown by.
Weather right now where I am has been typical for this time of year-Rains&lighting coupled with hot days and very humid conditions
So of course I been looking everytime I go to the beach and today...surprise surprise
Pans in every nook and cranny

The more I looked the more I saw
They certainly have established themselves in this area

...aswell as some tasty Termitomyces further down the road for the pot

These are one of my favorite local edibles to look for
They are found scattered around termite ant mounds and are cultivated deep inside by the ants
The fruits typically appear after lighting storms
A very mystical and tasty mushroom!!

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Re: unknown pans [Re: Zifozonke]
    #27026756 -

Great stuff man! So what are these pans after all? Panaeolus zifozonkeii?

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Re: unknown pans [Re: ZenZone]
    #27026782 -

Beautiful shots, Ziffy!

Are those Pans in the first pic so tiny or are African pill bugs fucking enormous?

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Re: unknown pans [Re: Adas]
    #27026981 -

Beautiful pans! I swear the macro features of these look JUST LIKE the panaeolus cyanescens (copelandia) that i find. I wonder if these are an undescribed copelandia. Have you sent then for sequencing?


--------------------
Whoever appeals to the law against his
Fellow man is either a fool or a coward
Whoever cannot take care of himself without that law is both
For a wounded man shall say to his assailant
If I live I will kill you, if I die you are forgiven
Such is the rule of honor

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Re: unknown pans [Re: MentalPariah]
    #27026996 -

Zifo, wish these were as easy to fruit as the Wild Coast man.


--------------------
Cultivating Panaeolus cyanescens


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Re: unknown pans [Re: MentalPariah]
    #27027499 -

MentalPariah said:
Have you sent then for sequencing?



I have some and plan to run them soon.  I sent some gill fragments to another user who was successful in cultivating them.

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Re: unknown pans [Re: Alan Rockefeller]
    #27027611 -

Alan Rockefeller said:
MentalPariah said:
Have you sent then for sequencing?



I have some and plan to run them soon.  I sent some gill fragments to another user who was successful in cultivating them.



:wat:
From a gill fragment??
:mindexpanding:

How does that work? I assume the tissue was dry, right?
So how does one cultivate from dry tissue? Does it have to be gill tissue?
Or he obtained spores from the gill fragment?
I have so many questions I'm sorry.

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Re: unknown pans [Re: Alan Rockefeller]
    #27027655 -

Alan Rockefeller said:
MentalPariah said:
Have you sent then for sequencing?



I have some and plan to run them soon.  I sent some gill fragments to another user who was successful in cultivating them.



Oh ok! Well I look forward to the result


--------------------
Whoever appeals to the law against his
Fellow man is either a fool or a coward
Whoever cannot take care of himself without that law is both
For a wounded man shall say to his assailant
If I live I will kill you, if I die you are forgiven
Such is the rule of honor

Extras: Filter Print Post Top
Re: unknown pans [Re: ZenZone]
    #27027851 -

ZenZone said:
How does that work? I assume the tissue was dry, right?
So how does one cultivate from dry tissue? Does it have to be gill tissue?
Or he obtained spores from the gill fragment?
I have so many questions I'm sorry.




If the gills are clean enough, you can just throw them on agar and they fire up a culture.  Probably from the spore but it's also possible the gill mycelium (lamellar trama) revives.

If they aren't that clean, serial dilutions are done so the spores germinate in different spots on the agar dish than the mold, yeast and bacteria.

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Re: unknown pans [Re: Alan Rockefeller]
    #27027908 -

:borat:


--------------------
:hitler::mushroom2:angulospora:heart:subaeruginosa:heart:subsecotioides:heart:tasmaniana:mushroom2::hitler:


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Re: unknown pans [Re: RogerTheRetard]
    #27028424 -

@Adas
Haha ...well spotted there...to try put into perspective those fruits in that pic are all in the 3-4gram range and the pill bugs are around the size of a golf ball when rolled up

@Asura
And you know then....

@AlanRockefellar
Did you take a look at the samples/spores under the microscope??
If so did you note anything different//comparable to other pan varieties you have studied?

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Re: unknown pans [Re: Zifozonke] * 3
    #27028530 -

hello Shoomerites!


This is the unidentified pan species from Africa. *with 90% certainty


Substrate: Basic pine forest floor recipe.
Choir
Straw
Manure
Sand
Red Clay
Conifer compost
Gypsum

Casing: Azura's pan casing recipe
Peat
verm
Lime
Hydrated lime



The myc acts and looks like a mix between pan and mexicana on agar. The growth was like a mix of the two species as well. They took longer to colonize substrate(30 days) and pin(20 days) than pan cyans, but that could have been due to imperfect conditions. However once they started fruiting they would pin and abort with the same finickyness of pans. The biggest difference from pans though is the cap texture. From my experience, pans in general have an almost rigid cap that can easily break, where with cubes, mexicana etc the caps are much more pliable. The caps of this new species are somewhere in between.

I didn't end up getting more pictures because after this first flush they just kept fruiting like pans do so I just focused on getting prints for Alan to study. Also, I didn't end up saving enough for a type collection so I pulled the master culture back out to noc some bags with tomorrow. 

Potency? I didn't try them. FWIW, I can tell you that the cut area of the stems turned blue/black within 5 minutes, like a normal pan.

*Why 90% certainty? Well, In my attempt to cram my wine fridge with more nocced up bags a few of the bags' labels fell off. Luckily this is the only pan I had going pre November. To add to that, every other bag without a label fruited so I was able to identify the species and rule them out. Plus, this is the only species I had going that grow this fast once pinned, everything else is zapos, paupuanas, caerulescens etc...

If you have any questions feel free to ask. I will be checking this thread daily. Only daily because I am extremely busy right now <3

:shroomeryhead:


--------------------
My Trade Lists

Edited by mycorry (11/08/20 02:36 PM)

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Re: unknown pans [Re: Alan Rockefeller]
    #27028534 -

Alan Rockefeller said:
If the gills are clean enough, you can just throw them on agar and they fire up a culture.  Probably from the spore but it's also possible the gill mycelium (lamellar trama) revives.

If they aren't that clean, serial dilutions are done so the spores germinate in different spots on the agar dish than the mold, yeast and bacteria.



Thank you Alan! :bow2:

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Re: unknown pans [Re: mycorry]
    #27028535 -

mycorry said:
hello Shoomerites!


This is the unidentified pan species from Africa. *with 90% certainty


Substrate: Basic pine forest floor recipe.
Choir
Straw
Manure
Sand
Red Clay
Conifer compost
Gypsum

Casing: Azura's pan casing recipe
Peat
verm
Lime
Hydrated lime



The myc acts and looks like a mix between pan and mexicana on agar. The growth was like a mix of the two species as well. They took longer to colonize substrate(30 days) and pin(20 days) than pan cyans, but that could have been due to imperfect conditions. However once they started fruiting they would pin and abort with the same finickyness of pans. The biggest difference from pans though is the cap texture. From my experience, pans in general have an almost rigid cap that can easily break, where with cubes, mexicana etc the caps are much more pliable. The caps of this new species are somewhere in between.

I didn't end up getting more pictures because after this first flush they just kept fruiting like pans do so I just focused on getting prints for Alan to study. Also, I didn't end up saving enough for a type collection so I pulled the master culture back out to noc some bags with tomorrow. 

Potency? I didn't try them. FWIW, I can tell you that the cut area of the stems turned blue/black within 5 minutes, like a normal pan.

*Why 90% certainty? Well, In my attempt to cram my wine fridge with more nocced up bags a few of the bags' labels fell off. Luckily this is the only pan I had going pre November. To add to that, every other bag without a label fruited so I was able to identify the species and rule them out. Plus, this is the only species I had going that grow this fast once pinned, everything else is zapos, paupuanas, caerulescens etc...

If you have any questions feel free to ask. I will be checking this thread daily. Only daily because I am extremely busy right now <3

:shroomeryhead:



Wow! How cool is this? :takingnotes:

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Re: unknown pans [Re: mycorry]
    #27028536 -

Mycorry, the way they grow out with the hygrophanous caps and the striated margin. Seems like your grow only deepened the mystery :nowaydude:

They very much resemble a psathyrella... not that they are, just visually


--------------------
Whoever appeals to the law against his
Fellow man is either a fool or a coward
Whoever cannot take care of himself without that law is both
For a wounded man shall say to his assailant
If I live I will kill you, if I die you are forgiven
Such is the rule of honor

Extras: Filter Print Post Top
Re: unknown pans [Re: Zifozonke]
    #27028701 -

Zifozonke said:
@Adas
Haha ...well spotted there...to try put into perspective those fruits in that pic are all in the 3-4gram range and the pill bugs are around the size of a golf ball when rolled up



Holy shit son! Those are som big Pans, AND Pillbugs! You could keep them as pets. :laugh:

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Re: unknown pans [Re: Adas]
    #27028776 -

Awesome grow!!  Cant wait to see what you guys find out a name for these guys


--------------------
Back at it like a crack addict, with asthmatic lung disease

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