Myc_Hunt said:

When doing transfers I work in a sab. After cutting out my wedge I can't seem to get the wedge off my scalpel when placing it into the new dish. Ive tried mashing, twisting, pulling and pushing but it's just stuck.

My other hand is holding the lid to help limit contam from landing on my plates.

So this is the question

Should I drop the plate and use another tool to push the wedge off the scalpel? Or should I make the agar thinner? I've been using 2% and its been great but these transfers give me a lot of anxiety

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I find that the best transfer method for me is to cut all the pieces, with a good overcut, so it is free. . .

I stick my blade point just into the side of the pieceat the cut edge on a downward 30° angle, just the tiniest bit, as ya know agar is sticky shit. . .

I then drag gently on the receiving plate and for stubborn pieces cut into the receiver so the wedge falls off. . .

Instead of thinner agar, make it thicker so you can cut into the receiver completely leaving The transfer on the surface. . . Not sure that is described well. . . But it how I do it

Crackatoa said:

I bend a 90 degree on my scalpel tip, helps cut pick up and drop.

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Crackatoa said:

I bend a 90 degree on my scalpel tip, helps cut pick up and drop.

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tryptkaloids said:

I use clean quart ziplock bags and shove 3/4 in each inbetween the pour and inoculation. That way i only need to wrap once

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Crackatoa said:

The first time was accidental, after that I've been bending them. I've never used heat, it's usually after I've used it a couple times. I've gone to the #7 scalpel handle now.

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MuthaFudpucker said:

Love it. I bet that is great for transfers. I am still using exacto blades and am afraid that they are too hard and will shatter if I try to bend them. But I will give it a go. I have some scalpels in my wish list, so if the exactos fail, I know what to do next. thanks!

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