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Unfolding Nature: Being in the Implicate Order

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nichrome]
#26863830 - 08/05/20 07:55 AM (6 years, 3 months ago)

I'm guessing this isn't PIO myc, but look at that great c10 pattern! :awesomenod:

:awesomenod:

It's the same color as the agar or it's raised agar. And it's shiny...
Agar is; 10g 2% Telephone Agar, 7.5g 1.5% LME, 500mL 50% Oat broth, Red 5 drops
:shrug:

:shrug:

:popcorn:

( How wonderful, a night with electricity AND A/C! )

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Stipe-n Cap
Full retard

Registered: 08/04/12
Posts: 9,117
Loc: Canada

Re: AGAR ENVY! (Anything and All things agar!)

1
#26863850 - 08/05/20 08:16 AM (6 years, 3 months ago)

That looks like a nice streak of bacteria.

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Stipe-n Cap]
#26863868 - 08/05/20 08:38 AM (6 years, 3 months ago)

Why thank you kind Sir! :horrified:

:horrified:

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One of Us
Stranger

Registered: 03/12/12
Posts: 1,140
Last seen: 6 months, 20 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Inthepit]
#26865434 - 08/05/20 10:26 PM (6 years, 3 months ago)

Are these blobs or pins? Would it be worth anything to clone, or will it just make blobs if I do?

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Spirit-Crusher

Registered: 04/24/19
Posts: 360
Loc: PNW
Last seen: 2 months, 24 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: One of Us]
#26865518 - 08/05/20 11:14 PM (6 years, 3 months ago)

Does anyone have any ideas for my scalpel handles? They get way too hot in my bacti-cinerator. I was thinking of finding some silicone tubing or something. Anyone want to save me the frustration of trial and error?

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ruK
Stranger

Registered: 05/27/20
Posts: 83
Last seen: 5 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Spirit-Crusher]
#26865597 - 08/06/20 12:25 AM (6 years, 3 months ago)

Spirit-Crusher said:

Does anyone have any ideas for my scalpel handles? They get way too hot in my bacti-cinerator. I was thinking of finding some silicone tubing or something. Anyone want to save me the frustration of trial and error?

Been wondering the same. Just looking on amazon and there are things to try.

https://www.amazon.com/s?k=heat+resistant+wrap&ref=nb_sb_noss_2

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G4NAEBAED
Stranger

Registered: 07/11/20
Posts: 5
Last seen: 6 years, 23 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: One of Us]
#26865652 - 08/06/20 01:28 AM (6 years, 3 months ago)

Those look like primordia which can turn into pins. To continue growth and not risk them dying and rotting, I'd take from a section of agar where those aren't.

Edited by G4NAEBAED (08/06/20 02:19 AM)

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Bobbit
Even Stranger

Registered: 05/30/18
Posts: 1,252
Loc: The outsideround

Last seen: 1 year, 3 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Spirit-Crusher]
#26865760 - 08/06/20 04:31 AM (6 years, 3 months ago)

Spirit-Crusher said:

Does anyone have any ideas for my scalpel handles? They get way too hot in my bacti-cinerator. I was thinking of finding some silicone tubing or something. Anyone want to save me the frustration of trial and error?

I stopped using my bacti-cinerator after buying 10 scalpel handles. . .

Set up 10 blades on handles. . . Wrap each in a foil pouch. . . PC standing in a jar. . .

Now to whole handle is sterile. . . Cheap aliexpress handles are fine. . .

They can be used months after being sterilised if stored proper. . .

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Bobbit]
#26865828 - 08/06/20 07:17 AM (6 years, 3 months ago)

G4NAEBAED said:

Those look like primordia which can turn into pins. To continue growth and not risk them dying and rotting, I'd take from a section of agar where those aren't.

I really dig this picture, is there a way I can copy it to my Gallery?

Bobbit said:

I stopped using my bacti-cinerator after buying 10 scalpel handles. . .
Set up 10 blades on handles. . . Wrap each in a foil pouch. . . PC standing in a jar. . .
Now to whole handle is sterile. . . Cheap aliexpress handles are fine. . .
They can be used months after being sterilised if stored proper. . .

If I can't stop infecting fresh plates, I might try that but...
What kind of handles are you talking about? I'm using X-Actos (2).
I'm suspicious of the knurling on the handle being hard to keep free of contams.

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One of Us
Stranger

Registered: 03/12/12
Posts: 1,140
Last seen: 6 months, 20 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Inthepit]
#26865850 - 08/06/20 08:01 AM (6 years, 3 months ago)

I use #7 scalpel handles and they are perfect.

I used to use exacto but those handles get hot and retain heat like crazy. Making the switch to scalpels was like night and day.

I too was worried about the texture on the exacto handle, so I pcd them in foil packs like Bobbitt, and only used for one session before getting sterilized again. An that worked fine.

But, make the switch to actual scalpels and you certainly won't regret it

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: One of Us]
#26865882 - 08/06/20 08:45 AM (6 years, 3 months ago)

Thanks One of Us for the quick comeback. I know c10 has very particular scalpels.
Mebbe if I dare try, I could start a thread with a survey and peeps could weigh in with their favs.

On the other hand, there's a hella lotta stuff in search for scalpel...

Oh, and then it gets deeper with Vendor sponsers...

Well two popular ones, both available same place (FP) but I don't see it listed in Vendors.
One is Stamets fav and another is a one pieces spear which RR said one piece scalpels get too hot. :shrug:

:shrug:

I dunno what the fascination is with knives, but I just had to order the Stamets one!
Woo hoo, mailman, mailman, don't be late...

Edited by Inthepit (08/06/20 10:33 AM)

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MadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Inthepit]
#26865962 - 08/06/20 10:03 AM (6 years, 3 months ago)

If you want to copy that picture just open it and save to camera roll then add to your shroomery gallery

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: MadHatter333]
#26865977 - 08/06/20 10:13 AM (6 years, 3 months ago)

Woo hoo TIL a new trick, thanks! :hatsoff:

:hatsoff:

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Edmunter
Mr

Registered: 05/01/13
Posts: 5,728
Last seen: 3 months, 21 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Inthepit]
#26866034 - 08/06/20 10:59 AM (6 years, 3 months ago)

When I take a clone and it goes like this I always think it’s some kind of contamination. What du rekkon?

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Gastronomicus
3-0-G

Registered: 04/01/05
Posts: 9,746
Last seen: 12 days, 3 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Edmunter]
#26866093 - 08/06/20 11:41 AM (6 years, 3 months ago)

I think so too. I get very similar growth off of the clones I've taken. But the disorganization makes me think mold.

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Smoothcat
Renegade-master

Registered: 10/07/15
Posts: 1,256
Last seen: 1 year, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Gastronomicus]
#26866119 - 08/06/20 12:05 PM (6 years, 3 months ago)

Yeah no7 scalpel what I use, pretty sure it’s the same as c10

Also Ed, I’ve also just taken a Toc clone myself wink wink nudge nudge :wink:

:wink:

Oh yeah and a pesa one too just cleaning it up, it was super strong and chunky too never seen them soo blue

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ruK
Stranger

Registered: 05/27/20
Posts: 83
Last seen: 5 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Inthepit]
#26866140 - 08/06/20 12:17 PM (6 years, 3 months ago)

Inthepit said:

Well two popular ones, both available same place (FP) but I don't see it listed in Vendors.
One is Stamets fav and another is a one pieces spear which RR said one piece scalpels get too hot.

What does FP stand for?

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MTManiac
Stranger Danger

Registered: 01/09/14
Posts: 57
Last seen: 1 year, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ruK]

1
#26866263 - 08/06/20 01:17 PM (6 years, 3 months ago)

On to Day 6
I messed around with the pictures today a bit. There are a few more dishes showing growth :smile:

:smile:

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Edmunter
Mr

Registered: 05/01/13
Posts: 5,728
Last seen: 3 months, 21 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Smoothcat]
#26866394 - 08/06/20 02:22 PM (6 years, 3 months ago)

Smoothcat said:

Yeah no7 scalpel what I use, pretty sure it’s the same as c10

Also Ed, I’ve also just taken a Toc clone myself wink wink nudge nudge

Oh yeah and a pesa one too just cleaning it up, it was super strong and chunky too never seen them soo blue

:getstoned:

how are u finding their strength?

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Smoothcat
Renegade-master

Registered: 10/07/15
Posts: 1,256
Last seen: 1 year, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Edmunter]
#26866720 - 08/06/20 05:37 PM (6 years, 3 months ago)

:chugbeer:

Not tried the Toc yet but this particular batch of pesa knocked me socks off to be fair

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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