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Re: AGAR ENVY! (Anything and All things agar!) [Re: EntheoGod]
    #26860877 -

Hard to see the myc on the plate but if it’s clean I’m sure they’ll be ok.

The wbs looks a little wet, I’d soak it less next time. I made mine too wet forever until one time I made a batch dry to use lc on and they had the fastest growth ever. Turns out they can seem dry but be perfectly hydrated.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26861022 -

Gotcha! I normally make them VERY dry so I’ve been working on getting my hydration down right. First time ever putting agar to the grains though. I did everything in a SAB and followed the proper teks. I’m excited about it. I think the bird seed will dry up over a few days. This was literally two hours after the seed was cool to the touch.

I’ve noticed in previous bird seed jars that it’ll appear pretty wet at first then be absorbed over the next few days. If it looks the same in a few days then I’d be not inclined to worry about my hydration even more. Definitely going to let them dry more next time though!

I apologize that it’s tough to see through the container but there was really no better way for me to take the pic :frown: 

Thanks a ton for the input! 👍

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Re: AGAR ENVY! (Anything and All things agar!) [Re: EntheoGod]
    #26861089 -

It’ll definitely absorb some of it over time but there’s a lot of burst grains in there which I usually get from soaking a little bit too much.

You’ll be amazed how much different the spawn is from agar once you get it down and everything’s clean.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26861105 -

I noticed the burst grains myself, it hasn’t ever caused any trouble for me. I’m more worried about the clean inoculate than I am about my grain prep 😅.

The only trouble I ever have is from dirty spawn 😓 I have recently switched from MS Syringe to grain and am now never putting ms syringes to grain ever again. After making the move to agar and seeing how dirty a syringe actually is, I’m blown away that it ever worked in the first place.😂

I’ll be doing a 3rd transfer of different agar later tonight. It’s the same culture I used to inoculate the jars you seen in the other post. Gonna see how different it looks after this 3rd transfer. Hoping for a ton of clean plates to store for future master jars. I’m already trying to comprehend how quickly this can expand from just a single master grain jar or a single clean culture.

Edited by EntheoGod (08/03/20 05:32 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: EntheoGod]
    #26861147 -

Something maaaaay be going wrong here...






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Re: AGAR ENVY! (Anything and All things agar!) [Re: AlsetAlokin]
    #26861372 -

Photos closer to the dish would be helpful


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"When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Sherlock Shrooms]
    #26861403 -

Plate 1


Plate 2


Plate 1 has something bad going on.
Plate 2 is suspect, the stringy yellow is freaking me out and is visible on all plates.

From MSS to MEA with some very clumpy charcoal.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AlsetAlokin]
    #26861576 -

That's all bad.


--------------------
Question everything, it's very responsive.


Culture Storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
    #26861653 -

That was my initial thought. Looks like you've successfully isolated a contaminant on most of those plates! Plate 1 has obvious blooms of some other mold as well..


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"When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Sherlock Shrooms]
    #26861662 -

Pretty sure all of my syringes came from an enemy that used them as toilet paper.

Getting plenty of agar practice along the way, though.

I know this isn't a contamination thread but if anyone can identify what it is, it'd be appreciated.

Let them go for the hell of it, or toss it quickly?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AlsetAlokin]
    #26861848 -

Keep them sealed and watch what they do if you want to. Be sure to leave them sealed when you throw them away later.


--------------------
"When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Sherlock Shrooms]
    #26862805 -

Done hijacking this thread, my apologies.
Last update on these plates, retiring them before they break free.
Whatever it is, it's fast, stringy, yellow, and reaching up.







Thanks for the feedback, questions, and insight.
Better luck next time and I learned a lot.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AlsetAlokin]
    #26862905 -

Looks like something I had that colonized most of a jar of grain overnight. Wish I'd seen it on agar first!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AlsetAlokin]
    #26862916 -

AlsetAlokin said:
Done hijacking this thread, my apologies.
Last update on these plates, retiring them before they break free.
Whatever it is, it's fast, stringy, yellow, and reaching up.







Thanks for the feedback, questions, and insight.
Better luck next time and I learned a lot.



Uhh mold? Did you invert the colors? Toss those science projects.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #26862936 -

Hello everyone.
First time trying agar. I poured some plates, left them out for a bit for any contams so grow and threw them out.

These are two of the faster growing ms syringe of Treasure Coast and are the size of a nickle or bigger.

How do they look?

Should I start isolating now since it was from a syringe, or wait?

Suggestions on good sectors to harvest? I want to get a few.

Thanks in advance everyone :smile:

They are on Day 4


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MTManiac]
    #26862951 -

I'd wait a day or so until it grows out a bit but looking good so far:thumbup:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #26863038 -

mushboy said:
I'd wait a day or so until it grows out a bit but looking good so far:thumbup:



Thanks!

I'm a bit excited to finally have the time and space to do things propper

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MTManiac]
    #26863181 -

Keep an eye on the piece at 2 oclock in the second picture. Looks like a good spot to transfer. Looks like a winner.


--------------------
Question everything, it's very responsive.


Culture Storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
    #26863239 -

Nichrome said:
Keep an eye on the piece at 2 oclock in the second picture. Looks like a good spot to transfer. Looks like a winner.



Good eye. Looking back at yesterday both 2 oclock and 3 oclock had some nice little wedges


Day 1 the first couple strands were at 10 oclock.

Three good spots is a great place to begin. Once they get a little bigger :smile:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MTManiac]
    #26863570 -



Hoping someone with experience can weigh in on this ape plate. This is T3. Wtf am I looking at? This variety confuses the shit out of me and I can't get clean white myc to save my life. I started my second swab in hopes of getting better results. Is this plate just contam ridden or is this what weak ape myc looks like..? Agar recipe is 15g LME/20g agar per 1000mL w/ Ampicillin.

Edited by ruK (08/05/20 12:36 AM)

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